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Gliocladium spp. strain for suppressing botrytis cinerea pers

A technology for tomato botrytis cinerea and Slime mold, which is applied in the direction of chemicals, fungi and fungicides for biological control, and can solve problems such as endangering the safety and health of humans and animals, shortage, pollution of agricultural products and the environment, etc.

Active Publication Date: 2010-02-10
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the lack of tomato resistant source materials against Botrytis cinerea, it is difficult to breed resistant varieties of Botrytis cinerea through conventional methods
For a long time, the control of Botrytis cinerea has mainly relied on the use of chemical agents such as carbendazim and saccharin; however, due to the rapid emergence of bacterial resistance, its control effect has been greatly reduced, and it has seriously polluted agricultural products and the environment, endangering the safety and health of humans and animals

Method used

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  • Gliocladium spp. strain for suppressing botrytis cinerea pers
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  • Gliocladium spp. strain for suppressing botrytis cinerea pers

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specific Embodiment approach 1

[0022] Embodiment 1: The Gliococcus strain that suppresses Botrytis cinerea in this embodiment is Bionectria ochroleuca WY-1 (Bionectria ochroleuca WY-1), which is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC No. 1977.

[0023] G. pink WY-1 of the present invention can be very good on potato dextrose agar (PDA) medium, oatmeal agar medium, Gao's No. 1 medium, cornmeal peptone medium, carrot medium or sporulation medium. Good growth and sporulation, the most suitable medium is PDA medium. Wherein the corn meal peptone medium (1000ml) is made up of 30g corn meal, 20g peptone, 20g sucrose, 18g agar and the distilled water of remainder. Carrot culture medium (1000ml) consists of 200g carrots, 20g glucose, 18g agar and the balance of distilled water. Sporulation medium (1000ml) is made of 1.0g yeast extract, 1.0g beef extract, 2.0g tryptone, 10.0g glucose, 0.1g ferrous sulfate ...

specific Embodiment approach 2

[0065] Specific embodiment 2: The β-1,3-glucanase gene fragment in Gliococcus pinkum WY-1, which inhibits Botrytis cinerea in this embodiment, is shown in SEQ ID NO: 1.

[0066] In this embodiment, G. pink WY-1 is activated with PDA solid medium, and then transferred to the enzyme-producing medium for culture. Take 100 mg of G. pink WY-1 mycelium to extract RNA and transcribe it into cDNA, and pass GenBank Compared with the β-1,3-glucanase genes of other known fungi, the homology was compared with the Clustalw method, and then the most conserved amino acid sequence was found, and two pairs of degenerate primers were designed according to the conserved region, The β-1,3-glucanase gene fragment was amplified by RT-PCR and sequenced, and the GenBank accession number was DQ975304.

[0067] It was found that M. rosea WY-1 of this embodiment contains the β-1,3-glucanase gene. 100ml of the enzyme-producing medium consists of 3g laminarin, 0.3g NaNO 3 , 0.1g K 2 HPO 4 , 0.05g KCl,...

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Abstract

The invention discloses a gliocladium spp. strain for suppressing botrytis cinerea pers and relates to the gliocladium spp. strain. The invention provides a new strain of gliocladium spp., which can be used for suppressing the botrytis cinerea pers and have the effect of suppressing a plurality of other fungal diseases, thereby laying a material foundation for biological control of the botrytis cinerea pers and culture of new species for anti-botrytis cinerea pers by utilizing the biological technology. The gliocladium spp. strain for suppressing the botrytis cinerea pers is gliocladium roseumWY-1 which is collected in China General Microbiological Culture Collection Center with the collection No. of CGMCCNo. 1977. The gliocladium roseum WY-1 can significantly suppress pathogens of the botrytis cinerea pers, fulvia fulva, phytophthora parasitica, verticillium dahliae of tomato, fusarium wilt of tomato, valsa sordida nit, poplar fusarium wilt and blackcurrant leaf spot, and suppress the growth of mycelia of the pathogens.

Description

technical field [0001] The present invention relates to a slime mold strain. Background technique [0002] Botrytis cinerea is a worldwide tomato disease and the main disease of tomato. Botrytis cinerea mainly damages flowers and fruits, and can also damage leaves and stems; young fruits are more seriously infected, stigmas and petals are mostly infected first, and then transfer to fruits; fruits mostly spread from the fruit stem to the fruit surface; It is off-white, soft rot, and the disease grows a large amount of gray-green mold layer. In severe cases, the fruit falls off and becomes rigid after dehydration; the leaves are infected, mostly from the tip of the leaf, and the lesions expand inward in a "V" shape, water-stained at the beginning, Light brown, with inconspicuous alternate deep and light ring patterns. When it is wet, gray mold can be produced on the surface of the lesion, and the leaves will die; when the stem is infected, small water-stained spots will be pr...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01P3/00C12R1/645
Inventor 王傲雪杨依军李景富王迎春陈秀玲张丽莉
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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