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Gibberella and method for fermentation production of gibberellin GA4+7

A technology of gibberellum and fermentation medium, applied in the field of fermentation engineering, can solve problems such as few strains reported

Active Publication Date: 2013-03-20
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gibberella fermentation produces different types of gibberellins, among which GA 3 Mainly, for producing GA 4+7 There are very few reports on the dominant species

Method used

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Examples

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Effect test

Embodiment example 1

[0026] Plate culture: The Gibberellin strain NJYHWG32261 was connected to the PDA medium, cultured for 2 days at pH 6.0, temperature 32°C, and activated Gibberella colonies were obtained; the composition of the PDA medium was (g / L): Potato juice 250, glucose 10, agar 15;

[0027] Seed culture: Pick an activated Gibberella colony from the PDA medium and place it in a shake flask containing the seed medium. At pH 6.0, the culture temperature is 32°C, the rotating speed of the shaker is 160 rpm, and the seed liquid is obtained by culturing for 2 days ; The amount of seed culture medium is 10% of the volume of the shake flask; the seed culture medium is (g / L): glucose 30, NH 4 NO 32.0, MgSO 4 ·7H 2 O 1.0, KH 2 PO 4 ·3H 2 O1.0;

[0028] Fermentation culture: Inoculate the seed liquid in a shaker flask containing the fermentation culture liquid at a ratio of 5% of the volume of the fermentation medium, pH 6.0, culture temperature 32℃, shaker rotation speed 160rpm, and increase the shaker...

Embodiment example 2

[0030] Plate culture: The Gibberella spp. NJYHWG32261 strain was cultured on the PDA medium, cultured at pH 6.5, the culture temperature was 28 ℃, and cultivated for 3 days to obtain activated Gibberella colonies; the composition of the PDA medium was (g / L ): Potato juice 150, glucose 30, agar 20;

[0031] Seed culture: Pick 2 activated Gibberella colonies from the PDA medium and place them in the shake flask containing the seed medium. At pH 6.5, the culture temperature is 28°C, the rotating speed of the shaker is 180 rpm, and the seed solution is cultured for 3 days. ; The amount of seed culture medium is 15% of the volume of the shake flask; the seed culture medium is (g / L): glucose 40, NH 4 NO 3 1.0, MgS O 4 ·7H 2 O 0.8, KH 2 PO 4 ·3H 2 O0.5;;

[0032] Fermentation culture: Inoculate the seed liquid in a shaker flask containing the fermentation culture liquid at a ratio of 8% of the volume of the fermentation medium, pH 6.5, culture temperature 28°C, shaker rotation speed 180rp...

Embodiment example 3

[0034] Plate culture: The Gibberellia strain NJYHWG32261 was cultured on a PDA medium at pH 7.0 and a culture temperature of 26°C for 4 days to obtain activated Gibberella colonies; the composition of the PDA medium was (g / L) : Potato juice 200, glucose 20, agar 18;

[0035] Seed culture: Pick an activated Gibberella colony from the PDA medium and place it in a shake flask containing the seed medium. At pH 7.0, the culture temperature is 26°C, the rotating speed of the shaker is 200 rpm, and the seed solution is obtained by culturing for 4 days; The volume of the seed culture medium is 20% of the volume of the shake flask; the seed culture medium is (g / L): glucose 50, NH 4 NO 3 1.5, MgSO 4 ·7H 2 O 1.2, KH 2 PO 4 ·3H 2 O0.8;;

[0036] Fermentation culture: inoculate the seed liquid in a shaker flask containing the fermentation culture liquid at a ratio of 10% of the volume of the fermentation medium, pH 7.0, culture temperature 26℃, and shaker rotation speed 200rpm; after fermentati...

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Abstract

The invention discloses a gibberella and a method for fermentation production of a gibberellin GA4+7. The method belongs to the field of fermentation engineering. The method comprises the following steps of plate culture, seed culture and fermentation culture, wherein in the fermentation culture, condition control is carried out. A mixed carbon source is added into a fermentation culture medium; and at a particular moment, the fermentation culture medium with the mixed carbon source is added with vegetable oil and a characteristic precursor and an oxygen flux of the fermentation culture medium with the mixed carbon source is increased so that a GA4+7 valence is improved. The gibberellin GA4+7 obtained by gibberella fermentation has a high valence and a low cost, is convenient for operation and is conducive to industrial production.

Description

Technical field [0001] The present invention belongs to the field of fermentation engineering, and mainly relates to a strain of GA that produces gibberellin selected by our laboratory. 4+7 Of Gibberella fermentation medium is optimized while the fermentation process is controlled to improve GA 4+7 Methods of potency. Background technique [0002] my country is a large farming country with thousands of years of history. In traditional planting, the output of grain depends to a large extent on the local sunshine, temperature, humidity and other climatic conditions. Using modern science and technology to break through environmental and geographical restrictions is the main means to solve the food crisis. Studies have found that when plant cells receive environmental signals such as temperature and light, they will produce low concentrations of active substances that can regulate plant physiological responses, and affect cell division, elongation, and differentiation. These active s...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P27/00C12R1/645
Inventor 胡永红李佼佼杨文革钱永根唐容容
Owner NANJING UNIV OF TECH
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