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Bacillus belesei BMF 03 and use and fermentation method thereof

A technology of Bacillus velesi and Bacillus, applied in the field of marine bacteria, can solve problems such as environmental pollution, pesticide residues in agricultural products, and few types of biological pesticides.

Active Publication Date: 2019-01-25
HUAIHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is aimed at the problems of environmental pollution, harmful organisms producing drug resistance and pesticide residues in agricultural products caused by the current application of chemical pesticides to prevent and control plant diseases. Insufficiency of existing technologies such as production needs, providing a new Bacillus velezensis BMF 03 strain with strong antibacterial effect and promising prospects for the development and application of biological pesticides and fertilizers

Method used

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  • Bacillus belesei BMF 03 and use and fermentation method thereof
  • Bacillus belesei BMF 03 and use and fermentation method thereof
  • Bacillus belesei BMF 03 and use and fermentation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Embodiment 1, Bacillus velezensis (Bacillus velezensis) BMF 03 bacterial strain antibacterial experiment:

[0085] 1 Materials and methods

[0086] 1.1 Test material

[0087] 1.1.1 Tested strains

[0088] Bacillus velezensis (Bacillus velezensis) BMF 03 strain was isolated from Lianyungang waters; the pathogenic fungi for testing (see Table 8) and the pathogenic bacteria for testing (see Table 3) were obtained from the Marine Microbiology Laboratory of Huaihai Institute of Technology (abbreviated as this experiment). room) to save.

[0089] 1.1.2 Medium

[0090] PDA medium: 200g potato, 20g glucose, 20g agar, 1000mL water;

[0091] PD medium: 200g potato, 20g glucose, 1000mL water.

[0092] BMF 03 strain fermentation medium: 0.25% sucrose, 1.5% soybean meal, MgCl 2 0.01%, MnSO 4 0.01%.

[0093] NA medium: peptone 10g, beef extract 3g, NaCl 5g, agar 15g, water 1000mL, pH 7.0.

[0094] NA liquid medium: peptone 10g, beef extract 3g, NaCl 5g, water 1000mL, pH 7.0....

Embodiment 2

[0120] Embodiment 2, Bacillus velezensis (Bacillus velezensis) BMF 03 produces antibacterial substance fermentation medium and shake flask fermentation condition optimization experiment:

[0121] 1. Experimental purpose: BMF 03 fermentation medium for producing antibacterial substances and optimization of shaking flask fermentation conditions

[0122] 2 Materials and methods

[0123] 2.1 Tested strains

[0124] Marine bacteria BMF 03 strain: It was isolated, purified and preserved in Lianyungang sea area by our laboratory. Fusarium wilt of cucumber: preserved and provided by this laboratory.

[0125] 2.2 Medium

[0126] (1) PD: 200 g of potatoes, 20 g of glucose, and 1000 mL of water.

[0127] (2) PDA: 200 g of potatoes, 20 g of glucose, 20 g of agar, and 1000 mL of water.

[0128] 2.3 Experimental Instruments

[0129] Table 10 Test main instruments

[0130]

[0131] 2.4 Preparation of seed solution

[0132] Inoculate the BMF 03 strain on the PDA slant medium and ac...

Embodiment 3

[0201] Embodiment 3, the culture medium and fermentation condition optimization experiment of Bacillus Veles BMF 03 solid-state fermentation edible fungus chaff:

[0202] 1. Purpose of the experiment: In order to further utilize the BMF 03 strain to develop microbial fertilizers and pesticides, the total number of bacteria and spore yield in the solid medium after fermentation were used as detection indicators, and single factor and response surface design were used to test the BMF 03 solid-state The fermentation medium and fermentation conditions were optimized.

[0203] 2 Materials and methods

[0204] 2.1 Test material

[0205] 2.1.1 Tested strains

[0206] Bacillus velezensis BMF 03 strain: It was isolated and preserved from Lianyungang sea area in our laboratory.

[0207] Methylotrophic Bacillus (Bacillus methylotrophicus) BMF 04 strain: It was isolated and preserved from Lianyungang sea area in our laboratory.

[0208] 2.1.2 Instruments and equipment

[0209]

[...

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Abstract

A marine-derived Bacillus velezensis (Bacillus velezensis) BMF 03 having a deposit number of CCTCC NO: 2017374 is disclosed. BMF 03 strain and its aseptic fermentation broth have inhibitory effect onplant pathogenic fungi, including apple rot fungi, grape white rot fungi, mango anthracnose fungi and so on. The aseptic fermentation broth of BMF 03 strain can be used as an active ingredient to prepare an antibacterial drug, and the bacteria is selected from the group consisting of Keratinobacteria, Bacillus subtilis and Ralstonia solanacearum. The invention also discloses a fermentation methodof aseptic fermentation broth of BMF 03. The invention adopts a plate confrontation method and an Oxford cup method to screen a new marine bacterial strain BMF-03 with strong bacteriostatic effect andwide bacteriostatic spectrum, and adopts strain morphological observation, physiological and biochemical tests and 16S rDNA sequence analysis to determine the species relationship of excellent resistant strains. At that same time, the bacteriostatic substance produce by the strain, the sporulation fermentation medium and the shake flask fermentation conditions were also study, and the promoting effect of the strain fermentation broth and the solid fermentation product on the growth of the cucumber seedling was clarified, which laid a foundation for the application of the strain.

Description

technical field [0001] The invention relates to a marine bacterium, in particular to a Bacillus velaisi BMF 03, and also relates to its use and fermentation method. Background technique [0002] Biological control has attracted more and more attention because of its environmental friendliness, no residue, and no drug resistance. To protect the environment, reduce the use of chemical pesticides, and ensure food safety, more excellent strains that have strong antibacterial effects and promote crop growth are needed. . The existing microbial strains used for the development of microbial pesticides are mainly derived from terrestrial environments, and the development and research of microbial strains from the ocean started relatively late. Marine bacteria grow and reproduce fast, oligotrophic, salt-tolerant, and have rich metabolites, which are unique to terrestrial microorganisms. With its advantages, it has become an important microbial resource for the biological control of ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N3/00A01N63/00A01P3/00A01P21/00C12R1/07
CPCA01N63/00C12N3/00C12N1/205C12R2001/07
Inventor 暴增海陈茹李欢曹雪梅万丹丹吴海霞马桂珍
Owner HUAIHAI INST OF TECH
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