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New application of B Salvianolic acid taken as cosmetics additives with functions of whitening skin, removing freckle and resisting wrinkle

A technology of salvianolic acid and total salvianolic acid is applied in the field of salvianolic acid as an additive for whitening, freckle-removing and anti-wrinkle cosmetics, which can solve problems such as economic losses, and achieve the effects of reducing the level of biosynthesis, strong activity, and good cell proliferation.

Active Publication Date: 2013-01-30
黑龙江源梦健康产业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At the same time, the freshness of fruits, vegetables, fish and shrimp is also an urgent problem faced by my country's agriculture and fishery. Some agricultural and aquatic products, such as lychees and live fish, are brought to my country every year due to the lack of timely and effective fresh-keeping methods. huge economic loss

Method used

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  • New application of B Salvianolic acid taken as cosmetics additives with functions of whitening skin, removing freckle and resisting wrinkle
  • New application of B Salvianolic acid taken as cosmetics additives with functions of whitening skin, removing freckle and resisting wrinkle
  • New application of B Salvianolic acid taken as cosmetics additives with functions of whitening skin, removing freckle and resisting wrinkle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Accurately weigh 0.05g of L-dopa, dissolve it with 0.1mol / L PBS (pH6.8), dilute to 10mL, keep away from light, and dissolve by ultrasonic. L-dopa solution is easy to oxidize and should be prepared immediately.

[0019] Dissolve 25KU of tyrosinase powder with 0.1mol / L PB (pH6.8) and make the volume to 250mL. Dispense the prepared tyrosinase solution into centrifuge tubes, seal and place in a -20°C refrigerator save.

[0020] Accurately weigh 10.00 mg of total salvianolic acid, and dissolve it in 10 mL of double distilled water to obtain a salvianolic acid solution. Three concentration groups of 1.0 mg / mL, 0.1 mg / mL and 0.01 mg / mL of the extract were finally obtained through stepwise dilution with double distilled water. Store at 4°C for later use

[0021] The frozen tyrosinase solution was placed in a 37°C water bath, and the solution in the tube was quickly thawed by shaking quickly. The thawed tyrosinase solution must be used up within 4 hours each time.

[0022] A...

specific Embodiment 2

[0035] Effect of salvianolic acid on tyrosinase activity of A375 and Hacat co-cultured cells

[0036] The A375 cells and Hacat cells in the logarithmic growth phase were respectively digested with 0.25% trypsin, centrifuged, and suspended in MEM culture medium. Mix the two cell suspensions at a ratio of 1:2, and adjust the cell density to 1.5×10 4 cells / mL inoculated in a 96-well plate, 200 μL per well, at 37°C and 5% CO 2 Incubate for 24h. Invert the culture medium, select 4 concentrations (400 μg / mL, 200 μg / mL, 100 μg / mL, 50 μg / mL) according to the results of the MTT experiment, 200 μL per well, set 6 duplicate wells in each group, continue to incubate for 48 h, wash with PBS for 2 For the second time, add 100 μL of 1% Triton-X solution to each well, quickly place it in a -80°C refrigerator for 30 minutes, take it out and thaw at room temperature to fully lyse the cells. Add 50 μL of 0.5% L-DOPA solution to each well, place the culture plate on a microplate shaker, shake ...

specific Embodiment 3

[0041] Effects of Salvianolic Acid on Melanin Synthesis in A375 and Hacat Co-cultured Cells

[0042] Mix A375 cells and Hacat cells at a ratio of 1:2, and adjust the cell density to 4.5×10 4 cells / mL inoculated in a 6-well plate, 2 mL per well, at 37°C and 5% CO 2Incubate for 24h. Aspirate and discard the culture medium, select 4 concentrations (400 μg / mL, 200 μg / mL, 100 μg / mL, 50 μg / mL according to the results of the MTT experiment, 2 mL per well, continue to incubate for 48 h, remove the culture medium, wash twice with PBS, aspirate and discard Culture medium, add 0.25% trypsin 1mL / well to digest, add 1mL MEM culture medium to stop digestion, make cell suspension, collect cell suspension into 15mL centrifuge tube, centrifuge, discard supernatant, add 100μL concentration of 1mol / LNaOH, 37℃ water bath for 1h, fully lyse cells and dissolve melanin particles. Add 400μL double distilled water to dilute, mix well, add 100μL / well to 96-well plate respectively, set 4 duplicate we...

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Abstract

The invention relates to new use of B Salvianolic acid. The B Salvianolic acid has a strong inhibition effect to tyrosinase, thereby being used for whitening skin, removing freckle, resisting wrinkle, and treating hyperpigmentation diseases, and being applicable to food additives. The inhibition of the B Salvianolic acid to the activity of the tyrosinase is detected, the B Salvianolic acid is very high in the activity of the tyrosinase, and is better in stability, and the B Salvianolic acid can effectively inhibit the generation of the melanin, and can be taken as the natural skin-whitening agent for cosmetics. Meanwhile, the B Salvianolic acid can more preferably promote the skin cell proliferation function, and has the functions of beautifying and resisting wrinkle, thereby being applicable to the cosmetics with the functions of beautifying and resisting wrinkle.

Description

technical field [0001] The invention relates to a new application of salvianolic acid as a cosmetic additive for whitening, freckle and anti-wrinkle. Background technique [0002] With the development of the economy and the improvement of living standards, people pay more and more attention to the natural whitening of the skin. "Skin like snow, condensed like fat" has always been the skin that the orientals advocate, and many people expect to get fair through the use of whitening skin care products. , shopping skin, which provides broad application prospects for various whitening compounds that inhibit the synthesis of skin melanin to achieve skin whitening effects, and promote the production and sales of this type of cosmetics. In the past, most of the whitening cosmetics were chemical drugs, but because of their side effects, they have been banned by many countries. The voice of "naturalization of components" and "return to nature" has become the trend of the cosmetics in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/537A61K31/216A61K31/343A61K8/97A61K8/49A61K8/37A23L1/272A61Q1/12A61Q19/00A61Q19/02A61P17/00A23L5/41A61K8/9789
Inventor 孙海峰杨菲菲丁常宏张宁朱天龙
Owner 黑龙江源梦健康产业发展有限公司
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