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Endophytic fungus in salvia miltiorrhiza bunge and application thereof

An endophytic fungus and tanshinone technology of Salvia miltiorrhiza, applied in the field of microorganisms, can solve the problems of difficult to realize industrialization, scattered distribution, long resource renewal cycle, etc., and achieve the effect of great application value.

Inactive Publication Date: 2012-09-19
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the Danshen resource area is vast, and a large number of artificial cultivations have been carried out; however, its distribution is scattered, the resource renewal cycle is long, and the artificial cultivation technology requires high requirements, and the quality of medicinal materials varies.
With the discovery of many pharmacological activities of tanshinone I and tanshinone IIA, the market demand for them is increasing, and the natural and artificial resources of salvia miltiorrhiza can not meet the market demand by directly harvesting
On the other hand, the chemical synthesis of tanshinone I and tanshinone IIA is difficult, and it is difficult to realize industrialization

Method used

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  • Endophytic fungus in salvia miltiorrhiza bunge and application thereof
  • Endophytic fungus in salvia miltiorrhiza bunge and application thereof
  • Endophytic fungus in salvia miltiorrhiza bunge and application thereof

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Embodiment 1

[0033] The endophytic fungus of Salvia miltiorrhiza of the present invention is a bacterial strain obtained from the root of Salvia miltiorrhiza cultivated in Shangluo, Shaanxi. The endophytic fungus of Salvia miltiorrhiza of the present invention is separated and obtained according to the following steps: washing with tap water for 30 minutes to remove sediment, and then washing with deionized water for 3 times. Cut the roots into 2cm-long root segments and follow the procedure for surface disinfection: 75% ethanol, 30s→1.3M sodium hypochlorite (3-5% available chlorine), 3min→75% ethanol, 30s→sterile deionized water wash 3 times . After the filter paper was blotted to dry the residual water, the root segment was cut into 5mm thick with a sterilized scalpel, and placed in PDA (potato 200g / L, glucose 20g / L, agar, 15g / L) containing 100mg / L penicillin for cultivation Incubate at 26±2°C on the substrate. The samples were observed for fungal growth every day. After 5-7 days, myc...

Embodiment 2

[0035] (1) Get the endophytic fungus strain of Salvia miltiorrhiza of the present invention, under aseptic conditions, pick a small amount of hyphae with an inoculation needle, insert it into a sterilized solid PDA medium plate, and activate and cultivate it for 72 hours at 26 ± 1°C . The morphology of endophytic fungi of Salvia miltiorrhiza on PDA plate medium is as follows: figure 1 shown.

[0036] (2) Take the strain after the activated culture, transfer it into the sterilized liquid culture PDA seed medium under aseptic conditions, and cultivate it on a shaker at 180 rpm at 26±1° C. for 72 hours to obtain seeds.

[0037] (3) The prepared PDA liquid culture medium was divided into 250ml Erlenmeyer flasks (about 100ml per bottle), sterilized, cooled and set aside. Under sterile conditions, inoculate the seeds according to the inoculation amount of 10%, and cultivate them on a shaker at 180 rpm at 26±1°C for 10 days. The morphology of endophytic fungi in Salvia miltiorrhiz...

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Abstract

The invention discloses an endophytic fungus in salvia miltiorrhiza bunge. The endophytic fungus is isolated and obtained from the living body of salvia miltiorrhiza bunge by adopting the endophytic fungus isolation and purification technology and is identified to be trichoderma atroviride through microbial taxonomy. The strain was collected in the China General Microbiological Culture Collection Center, with collection number being CGMCC No.4712.. Tanshinone compounds tanshinone I and tanshinone IIA are generated through liquid fermentation of the strain of the endophytic fungus in salvia miltiorrhiza bunge, so the endophytic fungus is an important microorganism for finding the new resources such as the tanshinone chemical components tanshinone I and tanshinone IIA.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to endophytic fungi of salvia miltiorrhiza and applications thereof. Background technique [0002] Tanshinone is the main fat-soluble component of Danshen, a traditional Chinese medicine that has the function of promoting blood circulation and removing blood stasis. Tanshinone I and Tanshinone IIA are its main active components. Tanshinone I has strong antibacterial activity, many times stronger than berberine; Tanshinone IIA has natural antioxidant effect, and is widely used clinically in cardiovascular and cerebrovascular diseases, kidney diseases, liver diseases, etc. At present, tanshinone I and tanshinone IIA are mainly derived from the root of Salvia miltiorrhiza Bunge, a plant of the Lamiaceae family. Although the Danshen resource area is vast, and a large amount of artificial cultivation has been carried out; however, its distribution is scattered, the resource renew...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P33/20C12R1/885
Inventor 韩婷明乾良秦路平张巧艳黄宝康张宏郑承剑黄芳
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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