Elephantopus scaber extract as well as preparation method and applications thereof in preparing antiviral drugs
The technology of an extract and C. chinensis, which is applied in the field of preparation of antiviral drugs, can solve the problems of toxic side effects, drug resistance, difficulty, virus antigen variability, antiviral drug resistance, antiviral drug resistance, and antiviral drug research and development difficulties. To achieve the effect of low cytotoxicity
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Embodiment 1
[0025] Get the dry root 200g of the herbal medicine Didancao of Compositae (collected from Guangdong, identified by Mai Zhenqiu, senior engineer of Guangzhou Medicinal Materials Company), pulverize, and use 2000mL of 95% ethanol aqueous solution to reflux extract 2 Once, each time for 1 hour, filter, combine the filtrates, concentrate under reduced pressure until there is no alcohol smell, and obtain the crude extract extract;
[0026] Weigh 500g of macroporous adsorption resin (D101), add 800mL of distilled water, stir evenly, remove the resin monomer or fragments by water flotation, and then place it at room temperature for 24 hours. After the resin is fully swollen, drain the resin, and then use ethanol Pack the column by wet method, wash the column with ethanol flow until the volume ratio of the effluent and water is 1:5 and the mixture does not become white turbidity, then fully rinse the column with distilled water until the effluent has no smell of ethanol, and place the...
Embodiment 2
[0029] Grind the dry root of 200g of Radix Echinacea, reflux extraction with 2000mL of distilled water for 3 hours, filter, concentrate the filtrate with a rotary evaporator, add an ethanol solution with a mass percentage concentration of 95% under stirring until the ethanol content is a mass percentage concentration of 80%, Stand still for 24 hours, filter the supernatant, recover ethanol under reduced pressure until there is no alcohol smell, and obtain the crude extract extract. The extract of the crude extract was purified by a macroporous adsorption resin column to obtain the extract 2 of Echinopsis chinensis, and the purification method was consistent with that of Example 1. Calculated by the weight of the dry product of the extract, the mass percent content of the total phenols in the G. chinensis extract is greater than 50%.
Embodiment 3
[0031] Grind 200g of the dried roots of Echinopsis chinensis, reflux three times with 2000mL of distilled water, each time for 1-3 hours, filter, and combine the filtrates to obtain the crude extract. The extract of the crude extract was purified by a macroporous adsorption resin column to obtain the extract 3 of Echinopsis chinensis, and the purification method was consistent with that of Example 1. Calculated by the weight of the dry product of the extract, the mass percent content of the total phenols in the G. chinensis extract is greater than 50%.
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