38 results about "Pedunculoside" patented technology

The invention discloses a method for simultaneously preparing high-purity syringin and pedunculoside. According to the method, the high-purity syringin and the high-purity pedunculoside are prepared by using holly barks as a raw material, adopting a solvent extraction method and a macromolecular resin adsorption method for extraction and separation and adopting re-crystallization, open ozone depleting substance (ODS) column chromatography or Se-phadex LH-20 column chromatography, liquid chromatography and the like. The purity of each of the syringin and the pedunculoside is over 90 percent, and the method is simple, feasible and suitable for industrialized production.

The invention discloses the comprehensive use of an ovateleaf holly bark medicinal material, which relates to method for extracting and separating from plants and use in field of medicines, in particular to a method for extracting syringin, pedunculoside, ovateleaf holly bark acid and ovateleaf holly bark total saponins from an ovateleaf holly bark medicinal material and use. In the invention, the syringing is prepared from water extract solution of the ovateleaf holly bark medicinal material, the pedunculoside and ovateleaf holly bark acid are prepared from medicine slag obtained after waterextraction from the ovateleaf holly bark medicinal material, the purities of the syringin, pedunculoside and ovateleaf holly bark acid are all over 98 percent, and the ovateleaf holly bark total saponins are extracted from the medicine slag obtained after the water extraction from the ovateleaf holly bark medicinal material. The ovateleaf holly bark total saponins can be used for preparing medicines for preventing and treating hyperlipemia, medicines for relieving myocardial ischemia and angina and medicines for treating inflammation and pain. The invention has the advantages that: four active ingredients can be obtained from the ovateleaf holly bark medicinal material and resources can be fully utilized; and 2, the raw materials are readily available, the process is simple, the quality is controllable and batch production can be performed.

The invention discloses a two-step simultaneous preparation method for pedunculoside and syringin and belongs to the technical field of natural product extraction and separation. The method comprises the following steps: adding 20-70% (volume fraction) of ethanol water into ovate leaf holly bark, performing reflux extraction, collecting an extracting solution, standing and filtering the extracting solution, and respectively collecting filtrate and residues; 2 drying the residues to obtain crude pedunculoside, and purifying the pedunculoside to obtain the pedunculoside; 3, performing column chromatography separation to the filtrate with macroreticular resin, taking an ethanol-water system as an eluting agent to conduct gradient elution, concentrating and drying elution components to obtain crude syringin, purifying the crude syringin to obtain the syringin. The method is simple and feasible, has a short separation time and high separating efficiency, the purity of the pedunculoside and the purity of syringin can both reach more than 98%, and the method is suitable for large-scale industrial production.

The invention discloses a method for simultaneously preparing high-purity syringin and pedunculoside. According to the method, the high-purity syringin and the high-purity pedunculoside are prepared by using holly barks as a raw material, adopting a solvent extraction method and a macromolecular resin adsorption method for extraction and separation and adopting re-crystallization, open ozone depleting substance (ODS) column chromatography or Se-phadex LH-20 column chromatography, liquid chromatography and the like. The purity of each of the syringin and the pedunculoside is over 90 percent, and the method is simple, feasible and suitable for industrialized production.

The invention discloses an ilicis routundae cortex medicinal composition for preventing and treating alcoholic liver damage, alcoholic fatty liver, non-alcoholic fatty liver and hyperlipidemia. The composition is extracted and refined from natural ilicis routundae cortex and is not chemically modified, and is characterized by comprising substances such as pedunculoside, syringin, rotundicacid andthe like. The invention also discloses a preparation method of the ilicis routundae cortex composition and application to preparation of a medicament for preventing and treating the alcoholic liver damage, the alcoholic fatty liver and the non-alcoholic fatty liver and lowering blood fat.

The invention provides an application of pedunculoside: pentacyclic triterpene glycoside compound in preparing a diabetic skin ulcer drug.Pedunculoside has the effects of promoting the proliferationsof fibroblast and vascular endothelial cell and promoting vascular endothelial cell migration so as to effectively prompt the healing of skin ulcers in diabetic rats, and can be used for treating diabetic skin ulcers.In addition, the invention also provides a pharmaceutical composition for treating diabetic skin ulcers; the pharmaceutical composition contains pedunculoside or a pharmaceutically acceptable salt thereof, and can be made into various dosage forms to cope with diabetic skin ulcers of different types and different disease stages.

A pedunculoside extraction method adopting holly as raw material includes steps of extraction and purification. The extraction step includes performing refluxed extracting and isolating on the holly raw materials, water and ethanol solution under condition of microwave at low temperature ranging from 10 DEG C to 20 DEG C to obtain extracting liquid, and concentrating to obtain pedunculoside extract; dissolving the pedunculoside extract by 70-75% ethanol, filtering through activated carbon with 100 meshes, collecting filtrate and adjusting solution pH to be equal to 6 to 7, adding NaCl to saturate, cooling to minus 4 DEG C to 10 DEG C to precipitate, filtering and collecting precipitate, and drying to obtain high-purity pedunculoside.

The invention discloses an ilicis rotundae cortex medicament composition for preventing such diseases as prostatitis and prostatic hyperplasia. The composition is obtained through extraction from barks of llex rotunda thunb. and refinement and is characterized by being formed by pedunculoside, syringin, rotundic acid and other substances. Pharmacological tests prove that the composition has the effect of preventing prostatitis, prostatic hyperplasia and the like. The composition has new application to preparing medicaments for preventing prostatic diseases. Pharmaceutic preparations of the composition are elaborated and can be any one of tablets, capsules, pills, granules, suspensions, dripping pills, ointments and rectal administration preparations such as suppositories.

A novel application of pedunculoside in preparation of anti-(hepatitis-B-virus) medicines is disclosed for the first time by the invention. The application includes an application of the pedunculoside in preparation of medicines inhibiting hepatitis B surface antigen secretion or hepatitis B virus E antigen secretion. In-vitro HepG22.2.15 cell pharmacological experiments prove that: the pedunculoside has good anti-HBV functions and has characteristics of a low effective concentration and low cell toxicity; the pedunculoside can inhibit HBsAg and HBeAg; when the concentration is 100 [mu]g / mL, the HBsAg inhibiting rate and the HBeAg inhibiting rate are 34.8% and 35.2% respectively; and an HBV inhibiting function of the pedunculoside is higher than HBV inhibiting functions of positive controls which are lamivudine, oleanolic acid and a ilex rotunda bark extract product.

A pedunculoside extraction method adopting holly as raw material includes steps of extraction and purification. The extraction step includes performing refluxed extracting and isolating on the holly raw materials, water and ethanol solution under condition of microwave at low temperature ranging from 10 DEG C to 20 DEG C to obtain extracting liquid, and concentrating to obtain pedunculoside extract; dissolving the pedunculoside extract by 70-75% ethanol, filtering through activated carbon with 100 meshes, collecting filtrate and adjusting solution PH to be equal to 6 to 7, adding NaCl to saturate, cooling to minus 4 DEG C to 10 DEG C to precipitate, filtering and collecting precipitate, and drying to obtain high-purity pedunculoside.

The invention relates to a preparation method of pedunculoside. The method includes the following steps of using the traditional Chinese medicinal material ovateleaf holly bark as the raw material, conducting heating reflux extraction through a 20%-70% ethyl alcohol aqueous solution, concentrating filtrate till no alcohol smell exists to obtain extract, dissolving and evenly mixing the extract in a 30%-70% ethyl acetate aqueous solution, standing overnight, conducting filtering to obtain filter residues, and drying the filter residues to obtain pedunculoside. Compared with the prior art, the method has the advantages of being simple in process, short in period, low in cost, high in product productivity and purity, suitable for large-scale industrialized production, and the like.

This invention relates to ilexin, drug combination of ilexin and its extraction method, which is used to treat CHD, angina pectoris, stroke, cardiac infarction and other cerebral vascular diseases. It uses ethanol for extraction, adds water into the extract, centrifuges to get the sediments and supernatant. Sediments are added 50-95% ethanol for extraction and filtration, the liquid is concentrated, placed and filtrated, and the sediments are dried to get a part of total saponin, the saponin is crystallized by ethanol to get a part of ilexin. The supernatant is enriched and centrifuged for the sediments, which is washed with appropriate water and crystallized by ethanol after dried to get another part of ilxin. Two parts of ilxin are put together and recrystallized for several times to get the pure ilxin, which content can be more than 98.0%.

The invention discloses a traditional Chinese medicine for dental surgery anesthesia and a preparation method thereof. The traditional Chinese medicine includes the following raw materials: calmus, rhizoma nardostachyos, radix angelicae pubescentis, pentapetes phoenicea, glaucescent fissistigma root, typhae pollen carbo, passion flower, corydalis tuber, rhizoma drynariae, large-leaved gentian, stellaria media, purslane, herb of pedunculoside, radix angelicae, cuttlebone, hemiphragma heterophyllum, rhaphidophora decursiva, cryptotaenia japonica, dye yam, ivy tree bark, fruit of kwangsi dutchmanspip, catmint, symplocos chinensis, Japanese cayratia herb and leonurus pseudomacranthus. The invention has the following benefits: the traditional Chinese medicine for anesthesia utilizes China's traditional Chinese medicine theory, is nonpoisonous, has no side effects, and has the advantages of good pain killing and blood blocking effects, quick effect and low cost; when the traditional Chinese medicine is in use, no injection is required, and all that is needed is to use dental tweezers to grasp a cotton ball to bring medicine drops into or wipe the periodontal pocket of a tooth to be extracted multiple times; when no pain is felt at the periodontal tissues, a dental surgery is implemented. The traditional Chinese medicine for dental surgery anesthesia is capable of eliminating patients' sense of fear, and is particularly suitable for children and patients who are afraid or sick of injection.