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Application of pedicel glucoside in the preparation of anti-diabetic skin ulcer medicine

A long-stemmed holly glucoside and anti-diabetic technology, applied in the field of medicine, can solve the problems such as the inability to show the active ingredient of leucanthin, the unclear effective component of ulcer, and the unclear therapeutic potential of diabetic skin ulcer.

Active Publication Date: 2021-01-15
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The holly medicinal materials currently on the market, such as Sijiqing and Jiubingying, etc., are often used in the folks to treat burns and lower extremity ulcers, but these medicinal materials also contain long-stemmed hollysides, syringin, syringic acid, β - Compositions such as balsamol, β-sitosterol and other triterpenoid saponins, the chemical composition is complex, and the active ingredients for treating ulcers are still unclear
In addition, according to the literature report (Determination of the content of catechin glucoside in Jubiying medicinal materials by high performance liquid chromatography, Specialty Research 2007, 4: 45-47), Jiubiying is the known medicinal material with the highest content of pelicin glucoside , its content in Jiubingying is only 8.8%, which cannot show that pelicin must be the active ingredient of Jiubingying in treating ulcers
In summary, for researchers in this field, the ulcer healing effect of pelicin, especially the therapeutic potential for diabetic skin ulcers is still unclear, and there is no one on the market that utilizes pelicin medicines for skin ulcers

Method used

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  • Application of pedicel glucoside in the preparation of anti-diabetic skin ulcer medicine
  • Application of pedicel glucoside in the preparation of anti-diabetic skin ulcer medicine
  • Application of pedicel glucoside in the preparation of anti-diabetic skin ulcer medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Proliferation-promoting effect of pedicel glucoside on fibroblasts

[0037] Take fibroblasts in the logarithmic growth phase and adjust the cell concentration to 1×10 5 cells / mL, inoculated in a 96-well culture plate, 100 μL per well, and routinely cultured in an incubator containing 5% CO2 at 37°C for 24 hours. After the cells were inoculated synchronously, the administration group was added with different concentrations of compounds, respectively 5 μg / mL, 12.5 μg / mL, 25 μg / mL, 50 μg / mL, 100 μg / mL, 200 μg / mL, and the blank control group was added with the same volume of DMSO .

[0038] After continuing to culture for 24 hours, try to add 20 μL of 5 mg / mL MTT solution to each well under the condition of avoiding light. After continuing to culture at 37°C for 4 hours, discard the mixture of medium and MTT in each well, add 150 μL of DMSO to each well, shake and mix at room temperature for 10 minutes to fully dissolve the crystals, and measure its absorbance ...

Embodiment 2

[0040] Example 2: Proliferation-promoting effect of catechin glucoside on human umbilical vein endothelial cells

[0041] Human umbilical vein endothelial cells in the logarithmic growth phase were taken, and the cell concentration was adjusted to 1×10 5cells / mL, inoculated in a 96-well culture plate, 100 μL per well, and routinely cultured in an incubator containing 5% CO2 at 37°C for 24 hours. After the cells were inoculated synchronously, the administration group was added with different concentrations of compounds, respectively 5 μg / mL, 12.5 μg / mL, 25 μg / mL, 50 μg / mL, 100 μg / mL, 200 μg / mL, and the blank control group was added with the same volume of DMSO . After continuing to culture for 24 hours, 20 μL of 5 mg / mL MTT solution was added to each well. After continuing to incubate at 37°C for 4 hours, discard the mixture in each well, add 150 μL of DMSO to each well, shake and mix at room temperature for 10 minutes, and measure the absorbance at 490 nm wavelength with an ...

Embodiment 3

[0043] Example 3 Determination of the effect of pedicel glucoside on promoting migration of human umbilical vein endothelial cells

[0044] Human umbilical vein endothelial cells were digested with trypsin and counted according to 5×10 4 Inoculated in a 24-well plate, added cell culture medium containing 10% fetal bovine serum, at 37°C containing 5% CO 2 Routine culture in the incubator for 24 h. When the cells grow to 90%, change the medium containing 1% fetal bovine serum to starve for 12 hours, use a sterile 200 μL pipette tip to make vertical scratches on the cell layer, establish an in vitro cell trauma model, and repeat with PBS Rinse until the scratched area is clean, add normal culture medium containing different concentrations of pelicin, the final concentration of the drug is 25 μg / mL, 50 μg / mL, 100 μg / mL, 200 μg / mL, and culture at 37 °C with 5% CO2 Routine culture in the box for 24h. At 0h and 24h, observe the migration of cells in each concentration intervention...

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Abstract

The invention provides an application of pedunculoside: pentacyclic triterpene glycoside compound in preparing a diabetic skin ulcer drug.Pedunculoside has the effects of promoting the proliferationsof fibroblast and vascular endothelial cell and promoting vascular endothelial cell migration so as to effectively prompt the healing of skin ulcers in diabetic rats, and can be used for treating diabetic skin ulcers.In addition, the invention also provides a pharmaceutical composition for treating diabetic skin ulcers; the pharmaceutical composition contains pedunculoside or a pharmaceutically acceptable salt thereof, and can be made into various dosage forms to cope with diabetic skin ulcers of different types and different disease stages.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of pedicel glucoside in the preparation of medicines for treating diabetic skin ulcers. Background technique [0002] Diabetes is a metabolic disease that seriously threatens human health, and diabetic skin ulcer is one of the most common complications of diabetes. Patients suffering from diabetic skin ulcers have recurrent ulcer wounds that do not heal for a long time. In severe cases, extremity gangrene, sepsis, or even amputation and death may occur. At present, the treatment of diabetic skin ulcers mainly relies on antibiotics, which can treat the infection of the ulcer, but cannot promote the healing of the ulcer. In the market, there is also a lack of drugs that can effectively promote ulcer healing. Therefore, the development of new drugs for promoting diabetic ulcer healing has broad application prospects and important social significance. [0003] Pel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7024A61P17/02A61P3/10
CPCA61K31/7024A61P3/10A61P17/02
Inventor 严鹏程毛琦琦赵敏方城燕王仲乐潘晓军
Owner WENZHOU MEDICAL UNIV
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