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Pedunculoside extraction method adopting holly as raw material

The invention relates to a technique for extracting holothrin glycosides and an extraction method, which are applied to the extraction and production of traditional Chinese medicine and the fields of medicine, and can solve the problems of difficult industrialization control, high production components, low efficiency and the like, and achieve the effects of easy instantaneous control, uniform extraction and increased efficiency.

Active Publication Date: 2015-04-01
安徽世茂中药股份有限公司
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  • Description
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  • Application Information

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Problems solved by technology

In the process of extraction, there will be changes in properties, low efficiency, low purity, high production components, and industrialization is difficult to control.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Soak 500g of Sijiqing raw material in 2.5 liters of 60% ethanol solution for 0.5 hours, reflux extraction under 400W microwave at 10°C for 10 minutes, separate and obtain the extract, concentrate to obtain pedicel extract; Dissolve the extract with 0.5 liter of 70% ethanol solution, filter through 100 mesh activated carbon, collect the filtrate, adjust the solution to PH=6 with 0.05mol / L hydrochloric acid solution, add NaCl to make it saturated, cool to -4°C to precipitate, and collect by filtration Precipitated and dried to obtain 2.3 g of high-purity catechin glucoside with a purity of 95.1%.

Embodiment 2

[0014] Soak 500g of Sijiqing raw material in 5 liters of 75% ethanol solution for 3 hours, reflux extraction under 3500W microwave at 20°C for 30 minutes, separate and obtain the extract, and concentrate to obtain pelicin extract; Dissolve the extract in 0.8 liters of 60% ethanol solution, filter through 100 mesh activated carbon, collect the filtrate, adjust the pH of the solution to 7 with 0.2mol / L hydrochloric acid solution, add NaCl to make it saturated, cool to 10°C to precipitate, and collect the precipitate by filtration , dried to obtain 2.2 g of high-purity catechin glucoside, with a purity of 95.7%.

Embodiment 3

[0016] Soak 500g of Sijiqing raw material in 4 liters of 70% ethanol solution for 2.5 hours, reflux extraction in 3000W microwave at 20°C for 20 minutes, separate and obtain the extract, concentrate to obtain pelicin extract; Dissolve the extract with 0.9 liter of 65% ethanol solution, filter through 100 mesh activated carbon, collect the filtrate, adjust the solution to PH=6 with 0.1mol / L hydrochloric acid solution, add NaCl to make it saturated, cool to 0°C to precipitate, and collect the precipitate by filtration , dried to obtain 2.5 g of high-purity catechin glucoside, with a purity of 96.5%.

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PUM

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Abstract

A pedunculoside extraction method adopting holly as raw material includes steps of extraction and purification. The extraction step includes performing refluxed extracting and isolating on the holly raw materials, water and ethanol solution under condition of microwave at low temperature ranging from 10 DEG C to 20 DEG C to obtain extracting liquid, and concentrating to obtain pedunculoside extract; dissolving the pedunculoside extract by 70-75% ethanol, filtering through activated carbon with 100 meshes, collecting filtrate and adjusting solution pH to be equal to 6 to 7, adding NaCl to saturate, cooling to minus 4 DEG C to 10 DEG C to precipitate, filtering and collecting precipitate, and drying to obtain high-purity pedunculoside.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a method for extracting pelican glucoside from Sijiqing as a raw material, and belongs to the technical field of extraction and production of traditional Chinese medicines. Background technique [0002] Sijiqing is a plant belonging to the holly family. Distributed in various places south of the Yangtze River in my country, often growing in sparse forests. Its roots and leaves can be used as medicine, and its main effects are clearing away heat and detoxification; It is clinically used to treat cold and fever, cough due to lung heat, sore throat, urination, dysentery and diarrhea. [0003] Pelicin glucoside is one of the main active ingredients in Sijiqing, which belongs to glycosides. In recent years, domestic and foreign scholars have made some progress in the field of extraction of pedicel glycosides. For example, the Chinese patent (CN102649805) discloses a method for pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00
Inventor 李运华李静王甫成王晓彬龚道锋陈刚高波
Owner 安徽世茂中药股份有限公司
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