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Development and application of wide spectrum influenza vaccine

A vaccine and broad-spectrum technology, applied in the field of vaccine development, can solve problems such as indistinguishability, achieve the effects of curbing the spread of the virus, solving insufficient production capacity, and reducing economic losses

Inactive Publication Date: 2007-08-15
河南省生物工程技术研究中心
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

HBc particles formed by excision of the C-terminal polypeptide are practically indistinguishable from those formed by the full-length HBc polypeptide

Method used

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Examples

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example 1

[0029] Example 1: The present invention fuses 24 amino acid residues of the extracellular region of influenza virus matrix protein M2 with the N-terminus of HBc molecule by means of genetic engineering to obtain virus-like particles for preventing influenza.

[0030] 1. Acquisition of HBc-M2 gene: According to the codon preference of Escherichia coli, the nucleotide sequence of 24 amino acid residues MSLLTEVETLTRNGWESKSSSSSD in the extracellular region of influenza virus matrix protein M2 was synthesized by artificial synthesis, and compared with The HBc antigen gene was spliced ​​to form the fusion gene HBc-M2.

[0031] 2. Construction of HBc-M2 prokaryotic expression vector: HBc-M2 gene and expression vector PET30 were respectively digested with NdeI, precipitated with ethanol, centrifuged, dried, and then digested with XhoI and electrophoresed. Cut out the HBc-M2 gene and the expression vector PET30 after double enzyme digestion under the ultraviolet transilluminator and co...

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Abstract

This invention relates to a development study and application method of a new gene engineering synthetic peptide vaccine, specially relates to development study and application method of broad spectrum influenza vaccine by fusing conserved sequence of influenza virus stroma protein M2 and hepatitis B virus core antigen. The vaccine can immunize body to resist attacks from different hypotype influenza virus.

Description

technical field [0001] The invention relates to the development and application method of a novel genetic engineering synthetic peptide vaccine, in particular to a vaccine development method utilizing a specific conserved sequence on influenza virus matrix protein M2 fused with hepatitis B virus core antigen to form virus-like particles. Background technique [0002] With the development of technology, knowledge about pathogenic genes, pathogenic mechanisms and host immune responses of various microbial pathogens has become more and more abundant. Many antigenic determinants (protective epitopes) that can stimulate hosts to produce protective immune responses sequence is determined. Protective epitopes generally have a small molecular weight, and used to be chemically coupled with high molecular weight carriers or used with adjuvants to increase their immunogenicity. However, with the rapid development of recombinant DNA technology, the protective epitope gene is fused with...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/145A61K39/39A61K9/16A61P31/16C12N15/09
Inventor 李晨阳李玉林陈小科李智涛
Owner 河南省生物工程技术研究中心
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