Porcine circovirus-II vaccine potency test method
A technology for porcine circovirus and an inspection method, which is applied in the field of porcine circovirus type 2 vaccine efficacy inspection, can solve the problems of expensive PCV2 challenge model establishment, time-consuming piglet challenge experiment method, large batch-to-batch variation of inspection results, and the like, Achieve significant differences in experimental results, short detection time, and improve efficiency.
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[0030] Detection of Porcine Circovirus Type 2 Inactivated Vaccine Efficacy
[0031] 1. Source of inactivated vaccine
[0032] The virus strain of PCV2 inactivated vaccine is PCV2-SH strain (preservation number CGMCCNo.2389). The PCV2-SH strain was inoculated into PK15 cells, and the virus antigen solution was harvested 5 days later as an inactivated vaccine.
[0033] The prepared inactivated vaccines were divided into three groups Ⅰ, Ⅱ, and Ⅲ, and their antigen contents were determined to be 10 5.0 TCID 50 / ml, 10 5.5 TCID 50 / ml, 10 6.0 TCID 50 / ml. Next, three groups of inactivated vaccines were used as samples to immunize mice to determine their serum antibody levels.
[0034] 2. Antibody detection in immunized mice
[0035] (1) Immunization of BALB / c mice
[0036] The healthy BALB / c mice aged 5-6 weeks were randomly divided into 4 groups, 5 mice in each group, and the antigen content of 10 5.0 TCID 50 / ml porcine circovirus type 2 inactivated vaccine immunizati...
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