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Mycobacterium tuberculosis combined antigen for diagnosing pulmonary tuberculosis

An antigen and application technology, applied in disease diagnosis, analytical materials, biological material analysis, etc., can solve the problem that tuberculosis patients are difficult to achieve diagnostic results

Active Publication Date: 2015-06-03
ACADEMY OF MILITARY MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these antigens or methods reported in previous studies are often difficult to achieve satisfactory diagnostic results in tuberculosis patients with negative sputum culture

Method used

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  • Mycobacterium tuberculosis combined antigen for diagnosing pulmonary tuberculosis
  • Mycobacterium tuberculosis combined antigen for diagnosing pulmonary tuberculosis
  • Mycobacterium tuberculosis combined antigen for diagnosing pulmonary tuberculosis

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Embodiment Construction

[0014] The purpose of the present invention is to provide an antigen combination with important application value in the diagnosis of tuberculosis, which induces a higher antibody level especially in patients with negative sputum smear (positive sputum culture).

[0015] Another object of the present invention is to provide a method for diagnosing pulmonary tuberculosis using combined antigens. The method for diagnosing pulmonary tuberculosis has high sensitivity and specificity for patients with positive and negative sputum smears.

[0016] The combination antigen provided by the present invention is based on the antigen combination of Rv3871, Rv3876 and Rv3879. In practical applications, the full-length sequences of the three antigens can also be used, and specific sequences containing B cell immunologically active epitopes can also be used, such as Rv3871: No. The sequence from amino acid 60 to amino acid 96 (SEQ ID NO.2); Rv3876: the sequence from amino acid 380 to amino ac...

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Abstract

The invention provides a combined antigen comprising three mycobacterium tuberculosis antigens and an application of the combined antigen to preparation of a reagent for diagnosing active tuberculosis. The combined antigen comprises Rv3871, Rv3876 and Rv3879. Corresponding antibody detection methods are established by the aid of the three antigens and used for detecting corresponding antibody levels in biological samples, and the three detected antibody levels are particularly higher in sputum smear negative / sputum culture positive tuberculosis patients. A pulmonary tuberculosis diagnosing method is established by the aid of the combined antigen, and a positive sample is defined in such a manner that antibody levels corresponding to two or more antigens reach a positive judgment value. Verification of a lot of clinical samples indicates that the diagnosing method has the high sensitivity and the high specificity for sputum smear positive / sputum culture positive, sputum smear negative / sputum culture positive and sputum smear negative / sputum culture negative tuberculosis patients.

Description

technical field [0001] The invention relates to the field of immunodiagnosis, especially the field of diagnosis of active pulmonary tuberculosis. Background technique [0002] Tuberculosis is still one of the major diseases that seriously threaten human health today. According to the data reported by the World Health Organization (WHO) in 2013, in 2012 alone, there were about 8.6 million new cases of tuberculosis in the world, and about 1.3 million deaths due to ZC nuclear disease. The prevention and control of tuberculosis remains a major challenge facing the world. [0003] Early and rapid tuberculosis diagnosis methods are of great significance for the prevention and control of tuberculosis. Currently, researchers have developed a variety of methods for TB diagnosis, including tuberculin skin test, sputum smear, sputum culture, and PCR. These methods have made a certain contribution to the prevention and control of tuberculosis. However, current diagnostic methods sti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569C07K14/35
CPCC07K14/35G01N33/5695G01N2800/12
Inventor 冯晓燕张贺秋刘志强修冰水杨锡琴
Owner ACADEMY OF MILITARY MEDICAL SCI
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