Modified new coronavirus S gene, recombinant plasmid and recombinant bacillus calmette guerin vaccine constructed by same and application of recombinant bacillus calmette guerin vaccine

Abstract

The invention discloses a modified new coronavirus SARS-CoV-2S protein, a recombinant plasmid and a recombinant bacillus calmette guerin vaccine constructed by the modified new coronavirus SARS-CoV-2S protein, and application of the recombinant bacillus calmette guerin vaccine, and belongs to the technical field of biological agents. Through modifying a partial sequence of gene promoter region and a partial sequence of gene terminator region, the modified new coronavirus S gene complete sequence can be smoothly expressed in an escherichia coli-mycobacterium tuberculosis shuttle plasmid pMV261 to obtain a recombinant plasmid pMVS; the recombinant plasmid pMVS is introduced into BCG through electric transformation, and recombinant BCG (rBCG) is obtained; the rBCG can successfully express S protein, arouse human immune response and induce antibody generation to prevent virus invasion, and the constructed rBCG is a subunit vaccine, has a better protection effect than parent BCG, can prevent or treat new coronavirus and mycobacterium tuberculosis, and has huge social benefits.

Description

Technical field [0001] The present invention relates to the field of biological agents, particularly recombinant plasmids, recombinant BCG field, said further SARS-CoV-2S gene and recombinant plasmids and recombinant BCG construct new crown and application of the virus after a transformation. Background technique [0002] Novel coronavirus (SARS-CoV-2) belonging to the subgroup B β genus coronavirus of which the outer envelope has projections flower crown structure S protein, plays the most crucial role in viral attachment, integration into the host cell, and . As molecular biologists of SARS-CoV-2S protein structure and function and vaccine research reorganization, the S gene as a new SARS-CoV-2 recombinant vaccine research has attracted extensive attention. Since the S protein SARS-CoV-2 exposed on the viral surface, involved in recognition, binding and the endocytosis process. It is designed as a vaccine, therapeutic antibody targets for the development of the key. [0003] As...

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Problems solved by technology

[0004] On the one hand, the above-mentioned vaccines are all specific vaccines against the new coronavirus, which can only be used against the new coronavirus and cannot prevent and / or treat other viruses at the same time. On the other hand, the safety of the above-mentioned vaccines for children is yet to be tested. Associated adverse reactions may occur if vaccinated in children

The new crown virus vaccine of this project mainly focuses on the prevention and treatment of new crown virus in children under six years old. At present, there is no corresponding vaccine in China, so there is a huge market space

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