38 results about "Regulon" patented technology

A vaccine against Mycobacteria tuberculosis (Mtb) is provided. The vaccine comprises a recombinant Bacille Calmette-Guerin (BCG) subunit-based vaccine in which one or more Mtb antigens and one or more Mtb resuscitation or reactivation antigens are overexpressed, and in which at least a portion of the DosR regulon is up-regulated. The vaccine is protective against active Mtb infection both pre- and post-exposure to Mtb, and thus prevents disease symptoms due to the recurrence of a latent Mtb infection.

A vaccine against Mycobacteria tuberculosis (Mtb) is provided. The vaccine comprises a recombinant Bacille Calmette-Guerin (BCG) subunit-based vaccine in which one or more Mtb antigens and one or more Mtb resuscitation or reactivation antigens are overexpressed, and in which at least a portion of the DosR regulon is up-regulated. The vaccine is protective against active Mtb infection both pre- and post-exposure to Mtb, and thus prevents disease symptoms due to the recurrence of a latent Mtb infection.

The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E. coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence in E. coli of a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.

A vaccine against Mycobacteria tuberculosis (Mtb) is provided. The vaccine comprises a recombinant Bacille Calmette-Guerin (BCG) subunit-based vaccine in which one or more Mtb antigens and one or more Mtb resuscitation or reactivation antigens are overexpressed, and in which at least a portion of the DosR regulon is up-regulated. The vaccine is protective against active Mtb infection both pre- and post-exposure to Mtb, and thus prevents disease symptoms due to the recurrence of a latent Mtb infection.

The invention relates to an immunogenic composition composed of secreted polypeptides derived from Campylobacter jejuni non-flagellar proteins that are coordinately expressed with the flagellar regulon. The invention also relates to a method of inducing an immune response to the non-flagellar protein polypeptides.

The invention relates to a fungus or a mushroom and to a method of producing it wherein the fungus / mushroom has an increased expression level of a polypeptide and / or has a decreased expression level of a polypeptide, wherein the polypeptide comprises an amino acid sequence that has at least 40% amino acid identity or similarity with a sequence selected from SEQ ID NO:1-200 and / or that has at least 50% amino acid identity or similarity with a sequence selected from SEQ ID NO:201-208.

A method is provided for producing an L-amino acid by culturing a microorganism belonging to the Enterobacteriaceae family and having the ability to produce an L-amino acid, in a medium to produce and accumulate the L-amino acid in the medium. The microorganism has been modified by introduction of a DNA fragment which includes a pho regulon promoter and a structural gene encoding an L-amino acid biosynthetic enzyme, which is ligated downstream of the promoter so that the gene is expressed by the promoter, and so that the activity of the L-amino acid biosynthetic enzyme is increased by the expression of the gene by the promoter. In this way, the L-amino acid that is produced in the medium can be collected. Furthermore, the phosphorus concentration in the medium is such that the expression of the gene by the promoter is induced.

An L-amino acid is produced by culturing a microorganism of the family Enterobacteriaceae which has the ability to produce an L-amino acid and which has been modified so as to increase the amount of expression of one or more genes selected from the evgA gene, gadE gene, or ydeO gene that encodes a transcription factor involved in the EvgAS two-component system regulon in a medium to produce and accumulate an L-amino acid in the medium or in cells, and collecting the L-amino acid from the medium or cells.

The invention described herein relates to a Haemophilus influenzae (H. influenzae) regulon encoding type IV pili. In particular, the invention relates to type IV pili from nontypeable H. influenzae (NTHi) and from H. influenzae strains a, b, c, e and f. The invention provides isolated H. influenzae pilus polynucleotides and polypeptides encoded by the polynucleotides as well as polynucleotides and polypeptides encoded by the polynucleotides involved in the assembly / disassembly of the structure. The invention also relates to uses of these polynucleotides and / or polypeptides including methods for eliciting an immune response to H. influenzae and methods of treating and preventing H. influenzae related pathological conditions.

The present invention provides an isolated polynucleotide comprising the kstD promoter from Rhodococcus erythropolis. The polynucleotide can very advantageously be used as a controllable transcription activator. Said controlling function can be provided by providing said isolated polynucleotide with a nucleotide sequence encoding a transcription regulator of said promoter. In the present invention, such a transcription regulator may be externally induced, such as by introduction of steroidal compounds. In an alternative embodiment of the present invention the isolated polynucleotide may comprise the kstR gene or a homologue or a functional part thereof as the transcription regulator of the kstD promoter.

The invention provides ribozymes and encoding nucleic acids having target recognition sequences that allow the ribozyme to target and cleave BRCA-1 regulators, resulting in upregulation of BRCA-1 in a cell. Also provided are nucleic acids encoding BRCA-1 regulators that contain the target sequences recognized by the ribozymes of the invention. Fragments of these nucleic acid and protein sequences also are provided. Further provided is a method for identifying a gene where the expression level is affected by a BRCA-1 regulator and the identity of several such affected genes. Still further provided is a method of identifying a compound that modulates the activity of a BRCA-1 regulator. Also provided is a method of treating cancer, comprising introducing a ribozyme selectively reactive with an RNA encoding a BRCA-1 regulator into a cancerous cell. The invention further comprises a method of detecting a neoplastic cell in a sample.

A vaccine for treating or preventing the establishment of latent tuberculosis infections is provided. The vaccine comprises a recombinant mycobacterium that overexpresses the transcription factor DosR, at a level sufficient to induce production of the dosR regulon genes or proteins. A host to whom the vaccine is administered mounts an immune response to the dosR regulon proteins and is thus protected from the establishment, persistence or reactivation of latent tuberculosis.

The invention discloses application of eIF4G protein to regulation and control of the tolerance of plants on ABA (abscisic acid). The application required for protecting substances used for inhibitingspecific gene expression in the plants comprises the following steps of regulating and controlling the tolerance of the plants on the ABA; breeding plant varieties with reduced tolerance on the ABA;regulating and controlling vivipary resistance of the plants; breeding plant varieties with increased vivipary resistance, wherein a specific gene is a gene encoding the eIF4G protein. The conditionsthat the eIF4G protein is an important negative regulator in ABA signal conduction, and the tolerance of the plants on the ABA can be obviously reduced by knocking out the eIF4G gene are found. The sustainable agriculture development requirement is conformed; important practical values and market prospects are realized in aspects of culturing new efficient ABA and adversity stress resistant varieties and the like.

The invention discloses the application of the eIFiso4G1 protein in regulating the tolerance of plants to ABA. The present invention claims to protect the application of substances used to inhibit the expression of specific genes in plants: regulating the tolerance of plants to abscisic acid; breeding plant varieties with reduced tolerance to abscisic acid; regulating the embryonic resistance of plants; breeding A plant variety with increased fetal germination resistance; the specific gene is the gene encoding eIFiso4G1 protein. The present invention finds that the eIFiso4G1 protein is an important negative regulator in ABA signal transduction, and knocking out the eIFiso4G1 gene can significantly reduce the tolerance of plants to ABA. The invention meets the requirement of sustainable agricultural development, and has important practical value and market prospect for cultivating new varieties with high efficiency and tolerance to ABA and adversity stress.