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Infectious bursal disease virus strain A11 and application thereof

A bursal disease and infectious technology, applied in the field of microorganisms, can solve problems such as difficulty in eradication, and achieve the effect of fast speed and strong immune protection effect.

Active Publication Date: 2016-07-27
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the biological characteristics of IBDV such as strong resistance, mutability, and the ability to carry and spread in a variety of birds, it is difficult to eradicate the disease

Method used

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  • Infectious bursal disease virus strain A11 and application thereof
  • Infectious bursal disease virus strain A11 and application thereof
  • Infectious bursal disease virus strain A11 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] (1) Isolation of epidemic strains of infectious bursal disease virus

[0026] 1 material

[0027] Infectious bursal disease disease materials J3 (2013), A4 (2013), S8 (2013), S9 (2014), A11 (2014), S14 (2015) and J17 (2015) are from the past three years (2013~ 2015) In chicken farms in Jiangsu (J), Anhui (A) and Shandong (S) (IBD vaccine-immunized chicken flocks) with clinical IBD disease, the bursa of Fabricius has swelling, bleeding and inflammatory exudates Wait for the disease. SPF eggs (purchased from Shandong Yufeng Agricultural Science and Technology Co., Ltd. non-specific pathogen-free breeder farm) were hatched into 9-day-old chicken embryos in the laboratory and raised to 6-week-old chicks in a clean animal room. The DF-EGFP-miR-9 cell line is a chicken embryo fibroblast cell line that highly expresses gga-miR-9 and eGFP genes (Ouyang Wei, et al. The chicken embryo fibroblast cell line that highly expresses gga-miR-9* .China, national invention patent number ZL2...

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Abstract

The invention discloses an infectious bursal disease virus strain A11 and application thereof, and belongs to the field of the microbial technology. Infectious bursal disease viruses are separated from infectious bursal disease vaccine immunity failure chicken flocks appearing in recent years, biological characteristic analysis is carried out on obtained virus isolate strains, and the infectious bursal disease virus strain A11 with good biological characteristics is screened out from the obtained virus isolate strains and is a currently-popular infectious bursal disease virus with super-strong virulence. The virulence of the virus is attenuated with a chicken embryo and cell continuous passage method to culture the low-virulence strain A11, the low-virulence strain A11 has super-strong reproductivity in cells, the virus yield in cells reaches up to 10<11> TCID50 / 0.1 ml, immune protection resisting infectious bursal disease viruses can be rapidly generated by immunizing chicks with the strain A11, immune protection can be achieved after 1 week, and the strain A11 has huge application value in developing new specific infectious bursal disease vaccines.

Description

Technical field [0001] The present invention relates to an infectious bursal disease virus A11 strain, which has excellent biological characteristics, high yield in cells, strong immunity of chickens and high speed, and belongs to the field of microbiology technology. Background technique [0002] Infectious Bursal Disease (IBD) is an infectious disease caused by Infectious Bursal Disease Virus (IBDV) that invades the lymphatic tissues of chickens, especially the central immune organ—the bursal of Fabricius. The disease not only causes the death of diseased animals, but also causes the body's immunosuppression, which reduces the body's immune defense ability and fails the vaccination. It occurs in every chicken-raising area in the world. [0003] Due to the biological characteristics of IBDV, such as strong resistance, easy mutation, and a variety of birds that can be carried and transmitted, it is difficult to eradicate the disease. In recent years, due to the widespread use of I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A61K39/12A61P31/14C12R1/93
CPCA61K39/12A61K2039/5254A61K2039/552C12N7/00C12N2720/10021C12N2720/10034
Inventor 王永山梅永杰欧阳伟诸玉梅潘群兴王晓丽孟凯夏兴霞毕振威王晶宇
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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