Intensified tubercle-resisting action of human interleukin 24
A technology of interleukin and pvax-1-il-24, applied in the direction of interleukin, cytokine/lymphokine/interferon, animal/human protein, etc. The effect of increasing survival time and improving pathological level
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[0034] The human interleukin 24 provided by the present invention has the nucleotide sequence shown in SEQ ID NO.1, which contains 601 bases of the human interleukin 24 protein molecule (please refer to the sequence list at the end of the article).
[0035] The present invention provides recombinant plasmid pVAX1-IL-24 of human interleukin 24, the construction of which includes amplifying and purifying human IL-24 cDNA from pcDNA3.1-IL-24 by PCR to obtain a purified PCR product, which is constructed into On the eukaryotic expression vector pVAX-1, specifically include the following steps:
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