Preparation of immuno-stimulation composition for hemolycin in monad

Abstract

An immunostimulating complex with stable and durable immune effect is prepared from hygrophilic monodas through liquid culturing, centrifugal separating of supernatant, treating it by saturated ammonium sulfate solution, suspending its deposit by PBS dialyzing to obtain toxic, concentrating, adding organic acid, adding Mega-10, treating at 37 deg.C for 4 hr, adding Quil A, cholesterol and lecithin, treating by organic acid, and dialyzing.

Description

technical field [0001] The invention relates to the fields of molecular biology and immunology, in particular to a preparation process of the hemolysin immunostimulatory complex in Aeromonas hydrophila. Background technique [0002] Aeromonas hydrophila (Aeromonas hydrophila, Ah) belongs to Vibriaceae Aeromonas genus, is a pathogenic bacterium of humans, fish, amphibians, reptiles and mammals, and can cause fish bacterial sepsis, frog Diseases such as red leg disease and human diarrhea cause a large amount of economic losses every year. [0003] The pathogenic factors of Aeromonas hydrophila include exotoxin, extracellular enzyme, membrane adhesion factor, S layer protein, pili, transferrin and outer membrane protein. The exotoxin of the Ah pathogenic strain is an important pathogenic factor, and its research has been quite in-depth. Different researchers give different names to exotoxins, such as HEC toxin reported by Lu Chengping, enterotoxin reported by Rose et al., aer...

AI Technical Summary

Problems solved by technology

However, due to the large number of serotypes of Aeromonas hydrophila, there are differences in immunogenicity between strains, and the complex antigen structure of Aeromonas hydrophila makes it difficult to prepare combined vaccines. The antigenicity of whole vaccines often changes after inactivation. Furthermore, the whole bacterin vaccine has large particles and is less likely to be absorbed. The above factors have restricted the popularization of the whole bacterium inactivated vaccine.

[0005] The existing genetically engineered subunit vaccines retain the advantages of traditional subunit vaccines, such as: good safety, specific effective immune components, easy industrial production, and low production costs, but the immunogenicity is not strong

The use of adjuvants in subunit vaccines can significantly enhance the immunogenicity of subunit vaccines. Conventional adjuvants such as aluminum hydroxide and propolis, which are commonly used in clinical practice, have limited ability to improve immunogenicity, while Freund's adjuvant has a strong Synergistic effect, but its toxicity and side effects on animals are large, so it is banned from clinical use

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