Intensified tubercle-resisting action of human interleukin 24

Abstract

The present invention discloses the human interleukin-24 is shown in SEQ ID NO.1 nucleotide sequence, the sequence containing human interleukin-24 protein molecules of 601 base pairs. Human interleukin-24 recombinant plasmid pVAX1-IL-24, the building will include the use PCR IL-24cDNA from pcDNA3.1-IL-24 amplification and purification, in the purified PCR products, to really build nuclear expression vector pVAX-1, also includes recombinant plasmid pVAX1-IL-24 Construction and preparation steps. Construction of the present invention of pVAX1-IL-24 of the eukaryotic expression vector for in vivo expression in the IL-24 protein, a Th1-type cell-mediated immunity, its joint tuberculosis DNA vaccine Ag85B with immunization, than separate DNA vaccine Ag85B immune used with stronger anti-tuberculosis infection role, and enhance Ag85B resistance tuberculosis infection immune protective effect of IL-24 with that as an immune adjuvant, with the increase of anti-tuberculosis immune protection role.

Description

technical field [0001] The invention belongs to the fields of molecular microbiology and molecular and infection immunity, and in particular relates to a kind of human interleukin-24 (Interleukin-24, IL-24) enhancing anti-tuberculosis effect. Background technique [0002] Tuberculosis (Tuberculosis, TB) is an infectious disease that seriously endangers human health, caused by Mycobacterium tuberculosis (MTB) infection. At present, the global tuberculosis epidemic is still very serious. According to the World Health Organization's estimate, there were 8.8 million new tuberculosis cases in 2005, and 7.4 million cases were in Asia and sub-Saharan Africa. A total of 1.6 million people died from tuberculosis, including 195,000 people living with HIV. Among the 22 countries with a high burden of tuberculosis in the world, my country ranks second, second only to India [1] . Bacillus Calmette-Guerin (BCG) vaccination is currently the main measure to prevent tuberculosis, but larg...

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Problems solved by technology

However, it is difficult for DNA vaccines to exert a protective effect when injected alone, so how to improve their immune protection is imminent [4]

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