Infectious bursal disease virus a11 strain and its application
A bursal disease and infectious technology, applied in the field of microorganisms, can solve problems such as difficulty in eradication, and achieve the effect of fast speed and strong immune protection effect.
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[0025] (1) Isolation of epidemic strains of infectious bursal disease virus
[0026] 1 material
[0027] Infectious bursal disease materials J3 (2013), A4 (2013), S8 (2013), S9 (2014), A11 (2014), S14 (2015) and J17 (2015) are from the past three years (2013~ 2015) In chicken farms (IBD vaccine immunized flocks) in Jiangsu (J), Anhui (A) and Shandong (S), sick chickens with clinical IBD had swelling, hemorrhage, and inflammatory exudates in the bursa of Fabricius and other diseases. SPF eggs (purchased from Shandong Yufeng Agricultural Science and Technology Co., Ltd. Specific Pathogen Free Breeding Farm) were hatched into 9-day-old chicken embryos in the laboratory, and raised to 6-week-old chicks in a clean animal room. The DF-EGFP-miR-9 cell line is a chicken embryo fibroblast cell line that highly expresses gga-miR-9 and eGFP genes (Ouyang Wei, et al. Chicken embryo fibroblast cell line that highly expresses gga-miR-9* .China, National Invention Patent No. ZL20131056524...
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