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Attenuated live vaccine for preventing infection of toxoplasma gondii and application of attenuated live vaccine

A live attenuated vaccine and technology of Toxoplasma gondii, applied in the direction of anti-infective drugs, medical preparations containing active ingredients, protozoan antigen components, etc. The effect of strong immune protection

Inactive Publication Date: 2017-10-24
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the only live attenuated Toxoplasma gondii vaccine that can be marketed in the world is S48, but it is limited to the application of local goats and sheep

Method used

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  • Attenuated live vaccine for preventing infection of toxoplasma gondii and application of attenuated live vaccine
  • Attenuated live vaccine for preventing infection of toxoplasma gondii and application of attenuated live vaccine
  • Attenuated live vaccine for preventing infection of toxoplasma gondii and application of attenuated live vaccine

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Embodiment Construction

[0018] The present invention will be further described below. It should be noted that the following examples are based on the technical solution and provide detailed implementation and specific operation process, but the scope of the present invention is not limited to this example.

[0019] 1. Preparation of live attenuated vaccine

[0020] Inoculate HFF cells into a 25T cell culture flask, add 8ml 10% FBS DMEM medium, and inoculate at 37°C CO 2 Cultivate in the incubator, after the cells are completely overgrown, replace with 8ml 2% FBS DMEM medium, add 100ul PRUΔCDPK2 strain, 37°C CO 2 Culture in the incubator until Toxoplasma gondii escapes from the cells, collect the PRUΔCDPK2 tachyzoites that have just escaped from the HFF, pass the cell suspension through a 3 μm filter to remove cell debris, and then use sterile PBS to adjust the concentration of tachyzoites to 400 each / 200μL.

[0021] 2. Immunization of mice

[0022] Before immunization, the purchased Kunming mice w...

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Abstract

The invention discloses an attenuated live vaccine for preventing the infection of toxoplasma gondii and application of the attenuated live vaccine. The attenuated live vaccine is a toxoplasma gondii attenuated strain PRU delta CDPK2 tachyzoite suspension prepared by adopting PBS as a solvent. An immune dosage form of the attenuated live vaccine is determined, an immune inoculation program and an inoculation amount of the vaccine are ascertained, the attenuated live vaccine has higher immunity protection effect for the acute infection, chronic infection and congenital infection of the toxoplasma gondii by virtue of an animal experiment, not only can prevent the normal infection of the toxoplasma gondii, but also can effectively prevent the vertical propagation of the toxoplasma gondii by virtue of a mother-infant route, is attenuated live vaccine with great application value, can be used for preventing the chronic infection, acute infection and congenital infection of the toxoplasma gondii, and can effectively prevent the toxoplasma gondii.

Description

technical field [0001] The invention relates to the field of toxoplasma gondii live attenuated vaccine, in particular to an attenuated live vaccine for preventing toxoplasma gondii infection and application thereof. Background technique [0002] Toxoplasmosis is a zoonotic parasitic disease caused by Toxoplasma gondii. About one-third of the world's population is infected by it. Women infected with Toxoplasma gondii during pregnancy, Toxoplasma gondii can be vertically transmitted to the fetus through the placenta, causing miscarriage, premature birth, fetal malformation, stillbirth and hindering the normal growth of children, seriously endangering human reproductive health. Toxoplasma gondii can infect almost all warm-blooded animals. It is highly contagious, has a wide range of epidemics, and has a wide host spectrum. The disease can still be controlled by drugs in the acute phase. Once it turns into a chronic phase, there is currently no cure. It is imminent to develop ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/002A61K9/10A61P33/02
CPCA61K9/10A61K39/002A61K2039/522
Inventor 黄思扬王金磊李法财贺君君陈凯朱伟宁朱兴全
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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