The invention belongs to the field of
protein engineering, and particularly relates to duck adenovirus type I Penton
protein as well as a preparation method and application thereof. The inventor, by analysis, finds that the Penton
protein has the advantages of hydrophilicity, no transmembrane structural domain, no
signal peptide prediction and the like, and full-length expression is selected. A Penton target fragment is obtained through artificial synthesis after
sequence optimization, and the expression mode of the protein is
cell-
free protein expression. Compared with truncated expression ofantigen dominant epitopes of traditional Penton protein, the artificially synthesized and expressed Penton recombinant protein is high in accuracy, the neutrality of the protein is guaranteed, the trouble of high
mutation rate is avoided, and the application to the development of an
antibody detection technology is better facilitated. In addition, an expression
system has unique advantages of saving time and improving the total yield of soluble full-length protein. Furthermore, the expression
system is low in cost, large in expression quantity and high in
Western Blot detection
reactogenicity.