The invention relates to a gene probe of acinar soft tissue sarcoma and the application of the kit. The cloned fragments selected by the gene probe of the present invention are the combination of RP11-634L10, RP11-51H16 and RP11-475F12, and the combination of CTD-2311N12, RP11-416B14, CTD-2522M13, CTD-2312C1 and CTD-2248C21. The present invention overcomes the cumbersome and time-consuming RT-PCR and karyotype analysis methods, RNA degradation and many other factors that limit the application of RT-PCR, cannot obtain accurate diagnosis, and affect prognosis and postoperative treatment. The invention has high accuracy, high specificity, high success rate, strong fluorescent signal, simple operation, can be applied to paraffin sections, expands the scope of detection specimens, and establishes a new method for accurate, reliable and simple diagnosis of acinar soft tissue sarcoma. Created a precedent for the use of FISH to detect acinar soft tissue sarcoma.