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PCR reagent for detecting tumor medicine targets, and application of PCR reagent

A tumor drug, target technology, applied in the field of molecular biology, can solve the problem of liver cancer insensitivity

Pending Publication Date: 2020-09-22
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, clinical treatment shows that liver cancer is not sensitive to radiation therapy and traditional chemotherapy

Method used

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  • PCR reagent for detecting tumor medicine targets, and application of PCR reagent
  • PCR reagent for detecting tumor medicine targets, and application of PCR reagent
  • PCR reagent for detecting tumor medicine targets, and application of PCR reagent

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Embodiment 1

[0294] The present invention is illustrated by Sorafenib-treated human liver cancer Huh7 and liver cancer cell Huh7, but the present invention is not limited to these two types of cells, and the detection reagent of the present invention can be applied to liver cancer cell lines including SMMC7721, HCCLM3, etc., while It can also detect drug targets in clinical liver cancer tissues and paracancerous tissues of lung cancer, kidney cancer, gastric cancer, pancreatic cancer, ovarian cancer, glioma and other tumors.

[0295] The present invention cultures the human liver cancer cell line Huh7, then treats it with sorafenib (10uM) for 24 hours, and uses tumor drug target screening PCR reagents to detect the change of the core drug target molecule in the treated cell line.

[0296] The human liver cancer Huh7 used in the present invention was purchased from ATCC Company of the United States, the Shanghai Cell Bank, DMEM medium, and fetal bovine serum were purchased from Gibco Company...

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Abstract

The invention provides a PCR reagent for detecting tumor medicine targets, and an application of the PCR reagent. The PCR reagent comprises a PCR reaction primer for detecting relevant genes of ABCC1,AKT1, AKT2, ATF2, AURKA, AURKB and the like and internal reference genes and the like. Target tumor medicines are concentrated on a flat, and through primary real-time fluorescent quantitation PCR reaction, liver cancer Huh7 treated with medicines and untreated liver cancer Huh7 are used for contrast by core adjust and control molecules of reaction cells on medicine reaction, a possible way of the core signal molecules treated with the medicines is discussed, and a most direct evidence is provided for adjust and control of research on key proteins. The core signal molecules of the tumor medicine target can be quickly and accurately found from a transcriptional level, and a powerful tool is provided for discussion of a new targeted medicine mechanism, development of a targeted tumor inhibitor, and the like.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a PCR reagent for detecting tumor drug targets and an application thereof. Background technique [0002] Tumor is now the second leading cause of human death after cardiovascular and cerebrovascular diseases, which has brought serious burdens to people's health and social economic development. Overcoming tumors has been one of the goals that people have been striving for in recent decades. The target of tumor therapy is the basis of tumor treatment and the cornerstone of precise tumor treatment. Therefore, the screening of specific tumor drug targets is one of the hotspots of current scientific research. [0003] Taking liver cancer research as an example, in recent years, the rate of increase in the incidence of liver cancer ranks among the top among malignant tumors. However, clinical treatment shows that liver cancer is not sensitive to radiotherapy and tradition...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6851C12N15/11
CPCC12Q1/6886C12Q1/6851C12Q2600/158C12Q2600/136C12Q2561/113C12Q2563/107C12Q2545/101C12Q2531/113
Inventor 刘世海潘华政贺桂芳刘畅畅蔡铎
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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