Gene probe for alveolar soft part sarcoma and application of gene probe kit
A soft tissue sarcoma and gene probe technology, applied in the application field of fluorescence in situ hybridization probes, can solve problems such as time-consuming inappropriate, complicated technology, difficult diagnosis, etc., and achieve accurate, reliable and simple diagnosis, strong fluorescent signal, and high accuracy high effect
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Embodiment 1
[0039] Step 1: Preparation of Gene Probes:
[0040] Find the bacterial artificial chromosome (BAC clone) corresponding to the X chromosome TFE3 gene and ASPL gene through http: / / genome.ucsc.edu / , select the corresponding BAC clone fragments respectively, and control the complete coverage of the BAC clone fragments selected on the same side relative to the gene , and the fragments on the same side have a certain sequence overlap with each other. According to the above requirements, the BAC clone fragments on the ASPL gene were selected as RP11-634L10 (chr17: 79796813-79969288, the fragment length is about 172Kb), RP11-51H16 (chr17: 79931578-80097452, the fragment length is about 166Kb) and RP11-475F12 ( chr17: 80032268-80216871, the fragment length is about 185Kb); the BAC clone fragment on the TFE3 gene is CTD-2311N12 (chrX: 48713289-48923401, the fragment length is about 210Kb), CTD-2522M13 (chrX: 48420425-48602847, The fragment length is about 182Kb), RP11-416B14 (chrX: 485...
Embodiment 2
[0052] Example 2: Diagnostic kit for alveolar soft tissue sarcoma
[0053] Gene probe kit for alveolar soft tissue sarcoma, the kit is composed of probe hybridization solution and 4', 6-diamidino-2-phenylindole counterstaining agent, characterized in that:
[0054] (1) The combination of RP11-634L10, RP11-51H16, and RP11-475F12 located on the ASPL gene on chromosome 17, marked with a red fluorescent signal; Combination of CTD-2312C1 and CTD-2248C21, labeled as green fluorescent signal;
[0055] (2) The probe hybridization solution is prepared by mixing the probe with Human Cot-1 DNA, hybridization buffer, and purified water in proportion, and it needs to be stored at -20°C in the dark;
[0056] (3) 4′,6-diamidino-2-phenylindole counterstain is mainly used for nuclear staining.
[0057] (4) The combination of RP11-634L10, RP11-51H16 and RP11-475F12 located on the ASPL gene of chromosome 17 described in the Gene Probe Kit for Acinar Soft Tissue Sarcoma, the three clone fragmen...
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