36 results about "Epithelioid cell" patented technology

The invention belongs to the application field of fluorescence in situ hybridization probes and discloses a probe set for diagnosing Xp11.2 ectopic perivascular epithelioid cell neoplasm and application of the probe set. The probe set includes BAC clonal fragments RP11-918B12, RP11-916D13, RP11-416B14 and RP11-344N17. The probe set is capable of diagnosing the specificity and the sensibility of Xp11.2 ectopic PEComa 100%, is convenient, quick and reliable, and can ensure a high success rate. And moreover, the probe set can be used for preparing an Xp11.2 ectopic PEComa diagnostic kit.

The present invention provides methods and compositions for treating epithelioid cell tumors (such as a PEComa) by administering a composition comprising nanoparticles comprising an mTOR inhibitor and an albumin.

This invention discloses a method for separating and purifying skin caticular dry cells which adopts a self designed culture medium to suppress growth of fibrablasts and unform epithelioid cells grow around the tissue block to induce the cells to move out of the original micro-environment and gather again and grow on the new grown epithelioid cell layer. These clone grown cells are obviously different in morphology from its dependent trophocytes and can be selected with the help of dissection mirror and glass pins and nurtured for expansion independently. The acquired cells are tested by the generally acknowledged skin dry cells molecular label: ck19, integrin-beta1, P63, integrin-alpha 6 are positive which means the selected ones are purified skin dry cells.

The invention discloses a separation and purifying method of skin epiderm stem cell, which is characterized by the following: adopting self-designed culture medium to inhibit the growth of fiber cell to growing epithelial shaped cell around the tissue; inducing surface stem cell migrate original micro-environment; gathering to grow again on the new growing epithelial shaped cell. The cloned growth cell displays obvious shaped difference from epithelial shaped cell, which is defined purifying epiderm stem cell if the detection of epiderm stem cell molecular mark is CK19, beta1-integrin,P63, alpha-6-integrin, which is positive. The invention overcomes defect of traditional enzyme digestion method, which can separate and purify human and animal epiderm stem cell.

Disclosed is an autologous non-human mammalian model system derived from induced pluripotent stem (iPS) cells. Also disclosed are methods of differentiating non-human primate iPS cells, which can result in populations of cells enriched for SOX2+ or PDX1+ foregut-like cells, for CDX2+ hindgut-like cells, for CD34+ hematopoietic progenitor-like cells, or epithelial-like cells. Also disclosed is a non-human primate containing an autologous cell type of interest, which is differentiated in vitro from an induced pluripotent stem cell reprogrammed from a primary somatic cell. Methods of monitoring exogenously introduced cells within a non-human mammal are also disclosed.

The invention discloses a Pseudosciaena crocea brain cell line and its establishing method. The cell line is preserved in China Center for Type Culture Collection on Oct., 31, 2013, and has a preservation registration number of CCTCC NO:C2013156. The Pseudosciaena crocea brain cell line can be serially passed, can provide a large amount of the Pseudosciaena crocea brain cell line, and can be used for researching pathogenic characteristics, vaccines, gene functions and breeding; and the Pseudosciaena crocea brain cell line has excellent properties, is an epithelioid cell and is flatly anomaly triangular and polygonal. The Pseudosciaena crocea brain cell line has the characteristics of strong splitting, short passage time, easy digestion, good adherent rate and starvation resistance. The establishing method has strong repeatability, and the established cell line has a good stability.

The invention discloses a cell line for lowly-metastatic clear cell carcinoma of kidney of Han Chinese, namely LoMeT-ccRcc CCTCC No.C200910. The cell line can grow for a long time in vitro and be stably handed down, has epithelioid cell form, and has no contact inhibition. The cell is heteroploid, chromosome number and structure are aberrant. Immunohistochemistry shows masculine CA9 and CD133. A flow cytometry analyses and finds out that CA9 and CD133 expression is obviously enhanced in the condition that cell is handed down for many times. The cell line has lowly-metastatic trend after the cell is handed down continuously. The recovery rate of the cell line after being frozen is more than 80%, the recovered cell growth state accords with the original cell. The cell line of the invention is used for establishing a cell model for sieving and preparing medicines that is used for dialogising, preventing and treating clear cell carcinoma of kidney.

The invention relates to a method for generating cells, in particular to a method for generating autologous melanocytes based on 3D suspension iPS (induced pluripotent stem cells) and an application,and belongs to the field of biotechnology. According to the method, iPS are digested into single cells, embryoid bodies with uniform shape and size are generated by a three-dimensional culture plate,2D plane adherence is changed to 3D suspension culture in the early induction process 14 days before differentiation, so that the proportion of epithelioid cells in the differentiation process is reduced, differentiation efficiency of the melanocytes is improved, selection of the embryoid bodies before differentiation, the digestion time point of the single cells and culture medium components areoptimized, and the proliferation state of the melanocytes is improved; the obtained melanocytes have the characteristics highly close to those of normal melanocytes in vitro, and have characteristicssignificantly superior to those of normal melanocytes in the in-vivo transplantation process.

The invention relates to the field of medical bioengineering, and in particular relates to a B lymphocytoblast model realizing differentiation of epithelioid cells through lactic acid induction as well as a preparation method and an application of the B lymphocytoblast model. According to the B lymphocytoblast model, the normal B cells are converted into the immortalized LCL through EB viruses, then the immortalized B cells are cultured through application of lactic acid induction, and thus the cells realizing differentiation of epithelioid cells and being capable of secreting large amount of extracellular matrix are obtained; the migration capacity of the cells is enhanced, and a study tool is provided for researching the malignant evolution of B cell lymphomas.

The invention discloses a grass carp swim bladder epithelioid cells line and an application. According to the grass carp swim bladder epithelioid cells line with a preservation number of CCTCC No.C201443, Grass Carp Reovirus GCRV propagation amount in the cell line is obviously higher than that of the cell lines such as CIK, CO, PSF, FHM and EPC used for separating GCRV, cell pathology time is fast, and the cell line can better used in the researches of separation and breeding of Grass Carp Reovirus and grass carp hemorrhagic disease vaccine.

The invention provides a Cyprinus carpio var. jian cerebral line and application thereof. Through primary isolation on Cyprinus carpio var. jian cerebral tissues with pancreatin of an appropriate concentration and reasonable digestion conditions, Cyprinus carpio var. jian cerebral cells can be obtained, and through subculture, the Cyprinus carpio var. jian cerebral line CCB-J is established for the first time; the Cyprinus carpio var. jian cerebral line is mainly represented as epithelioid cells and can be passaged for more than 60 generations, thereby be high in research and application the fields such as virology, toxicology, physiology, molecular genetics and biology of evolution. The Cyprinus carpio var. jian cerebral line can be applied to virus identification and virus strain isolation, to preparation of Cyprinus carpio var. jian virus preventing or killing vaccines, as biological models of drug screening, preparation and evaluation and the like.

The invention discloses a culture medium and application thereof. The culture medium is a DMEM / F12 culture medium containing N2 supplement, retinoic acid and BMP-4. With the culture medium provided by the invention, stem cells can be effectively induced into epithelioid cells; then, the obtained epithelioid cells and mesenchyme of animal dental germs are subjected to recombination, culture and transplantation, so adamantoblast in a dental sample structure can be effectively obtained.

The invention provides applications of fusion proteins E-cadherin-Fc, VE-cadherin-Fc and VEGF-Fc. The invention relates to the field of cell differentiation. Specifically, the invention relates to newapplications of epithelial cell cadherin (E-cadherin)-Fc fusion protein and / or vascular endothelial cell cadherin (VE-cadherin)-Fc fusion protein and / or endothelial cell growth factor (VEGF)-Fc fusion protein in promotion of differentiation of stem cells to hepatocyte-like cells, endothelial-like cells, islet-like cells or biliary epithelioid cells.

The invention discloses a probe assembly for diagnosing ACTA2-MITF translocation perivascular epithelioid cell tumor and an application thereof. The probe assembly is formed by a BAC clone probe RP11-28G22 and a BAC clone probe RP11-963H1, wherein the BAC clone probe RP11-28G22 is located on one side of the centromere of ACTA2 and labeled with fluorescence of any color; the BAC clone probe RP11-963H1 is located on one side of the MITF telomere and labeled with fluorescence different from that labeled on the other side of the ACTA2 centromere. As the probe combination carry out in-situ hybridization on the basis of the paraffin-embedded tissue slice to detect the fusion and separation signals, the accuracy rate of diagnosis of such tumors can be greatly improved.

The invention provides a method for in-vitro isolated culture of chicken primary fallopian tube epithelial cells.The method includes the following steps that 1, tissue is aseptically separated from the neck of a fallopian funnel, close to a follicle, of a laying hen; 2, the tissue obtained after separation is digested by pancreatic enzymes and EDTA jointly, and the chicken fallopian tube epithelial cells are obtained; 3, at the temperature of 37 DEG C, the chicken fallopian tube epithelial cells are incubated for 2 h; 4, the cells obtained after incubation in the step 3 are cultured.The primary chicken fallopian tube epithelial cells obtained after isolated culture in the method are good in effect, high in growth speed and good in state; digestion time is short, the result is good, fewer parenchyma cells exist, contamination to fibroblasts is avoided, and healthy epithelioid cell populations can be obtained.

The invention relates to a method of establishing a rat pancreatic duct epithelioid stem cell system, and belongs to the field of animal cell (tissue) engineering. An adult rat pancreatic tissue is digested by collagenase, Dextron discontinuous density gradient centrifugation is carried out, serum culture is carried out in RPMI1640, pancreatic duct primary epithelioid stem cells are grown in a manner of being adhered to the wall, clone ring screening is carried out, epithelioid stem cells are purified, trypsin digestive juice is digested and gone down to the future generation to establish the cell system. The rat pancreatic duct epithelioid stem cell system established by adopting the method disclosed by the invention has the characteristics that the cell adherence is polygonal and epithelioid, and the cells grow in a clonal manner and are normal diploid cells; the rat pancreatic duct epithelioid stem cells express proliferating cell nuclear antigen, an octamer-binding transcription factor 4, stem cell key protein, keratin 19 and a neurogenic differentiation factor 2 on the protein level, have multiple differentiation potentials and are free of oncogenicity.

The invention relates to a high metastasis human breast carcinoma cell subline MDA-MB-231sci, which has biological and pathological features and special metastasis phenotypes. The biological features are as follows: the MDA-MB-231sci cells are typical polygonal epithelioid cells, have rich cytoplasm and big and round karyon, grow adherent to the wall with almost uniform size and are closely arranged with clear boundary. The pathological features are as follows: a tumor cell of a nude mouse in vivo metastasis part has the similar structure with the subcutaneous tumor cell, short metastasis time and high metastasis rate, wherein the metastasis time is shortened to 26-42 days; a lung metastasis rate is 100%, meanwhile, a part of axillary lymph node metastasis of an animal is performed. An establishing method for the high metastasis human breast carcinoma cell line comprises the following steps of: (1) establishing a human breast carcinoma orthotopic transplantation tumor model; (2) sieving an orthotopic transplantation lung targeted metastasis model; and (3) establishing and passaging a human breast and lung carcinomas targeted high metastasis cell line. In the invention, the success rate is improved by sieving surgical excision tumor of each generation in vivo and corresponding breast.

The present invention provides methods and compositions for treating epithelioid cell tumors (such as a PEComa) by administering a composition comprising nanoparticles comprising an mTOR inhibitor and an albumin.

The invention discloses toxicity-removing and tissue regeneration-promoting paste for treating ulcers and a preparation method thereof. The toxicity-removing and tissue regeneration-promoting paste is prepared from the following raw materials in parts by weight: 15-20 parts of coptis, 10-15 parts of Japanese ampelopsis, 5-10 parts of Japanese pagodatree twig, 11-17 parts of radix rehmanniae, 10-15 parts of peach leaves, 13-19 parts of bauhinia purpurea, 8-12 parts of phytolacca acinosa, 15-20 parts of ramie root, 5-10 parts of pangolin, 12-16 parts of polygala tenuifolia, 14-18 parts of chinquapin bark and leaves, 7-11 parts of cimicifuga foetida, 9-14 parts of honeysuckle, 12-16 parts of towel gourd root, 5-10 parts of frankincense, 8-12 parts of myrrh, 10-15 parts of lasiosphaera seu calvatia, 20-30 parts of mirabilite, 15-25 parts of calcite powder, 30-50 parts of calomel, 40-60 parts of rosin, 80-120 parts of white wax and 450-550 parts of sesame oil. The toxicity-removing and tissue regeneration-promoting paste has the efficacies of clearing heat and eliminating dampness, removing toxicity and promoting tissue regeneration, sterilizing and relieving itching, activating blood and eliminating stasis, relieving swelling and stopping pain, discharging pus and eliminating carbuncle, stopping bleeding and accelerating wound healing as well as promoting proliferation and division of neurogliocyte, regeneration of epithelioid cells and blood circulation, has unique and remarkable curative effects on treatment of ulcers, especially suppuration due to infection or wound surfaces which are not healed for a long period of time, and is quick in effectiveness, short in treatment course, high in cure rate, free of toxic or side effects and high in safety.

Provided is a selective method for inducing differentiation from pluripotent stem cells to enterocyte-like cells. Also provided is an excellent enterocyte-like cell expressing drug-metabolizing enzymes and drug transporters. More specifically, provided is an enterocyte-like cell having properties closer to those of primary enterocytes, which are difficult to acquire. The foregoing is achieved by adding an ALK5 inhibitor (SB431542), Wnt3a, and EGF to a culture system of definitive endoderm cells obtained by differentiation induction from pluripotent stem cells and extending a culture time. The foregoing is also achieved by introducing CDX2 gene and / or FOXA2 gene into the pluripotent stem cells or the definitive endoderm cells. The foregoing is also achieved by overlaying a basement membrane matrix on the enterocyte-like cells.

Provided is a selective method for inducing differentiation from pluripotent stem cells to enterocyte-like cells. Also provided is an excellent enterocyte-like cell expressing drug-metabolizing enzymes and drug transporters. More specifically, provided is an enterocyte-like cell having properties closer to those of primary enterocytes, which are difficult to acquire. The foregoing is achieved by adding an ALK5 inhibitor (SB431542), Wnt3a, and EGF to a culture system of definitive endoderm cells obtained by differentiation induction from pluripotent stem cells and extending a culture time. The foregoing is also achieved by introducing CDX2 gene and / or FOXA2 gene into the pluripotent stem cells or the definitive endoderm cells. The foregoing is also achieved by overlaying a basement membrane matrix on the enterocyte-like cells.

The invention provides a method for inducing human epidermal stem cells into corneal epithelioid cell differentiation in vitro. A specific cornea promoting peptide is used, so that the differentiationefficiency of the cornea epithelial cells can be obviously improved.

The invention relates to the field of medical bioengineering, and in particular relates to a B lymphocytoblast model realizing differentiation of epithelioid cells through lactic acid induction as well as a preparation method and an application of the B lymphocytoblast model. According to the B lymphocytoblast model, the normal B cells are converted into the immortalized LCL through EB viruses, then the immortalized B cells are cultured through application of lactic acid induction, and thus the cells realizing differentiation of epithelioid cells and being capable of secreting large amount of extracellular matrix are obtained; the migration capacity of the cells is enhanced, and a study tool is provided for researching the malignant evolution of B cell lymphomas.

The invention discloses a separation and purifying method of skin epiderm stem cell, which is characterized by the following: adopting self-designed culture medium to inhibit the growth of fiber cellto growing epithelial shaped cell around the tissue; inducing surface stem cell migrate original micro-environment; gathering to grow again on the new growing epithelial shaped cell. The cloned growthcell displays obvious shaped difference from epithelial shaped cell, which is defined purifying epiderm stem cell if the detection of epiderm stem cell molecular mark is CK19, beta1-integrin,P63, alpha-6-integrin, which is positive. The invention overcomes defect of traditional enzyme digestion method, which can separate and purify human and animal epiderm stem cell.

The invention belongs to the application field of fluorescence in situ hybridization probes and discloses a probe set for diagnosing Xp11.2 ectopic perivascular epithelioid cell neoplasm and application of the probe set. The probe set includes BAC clonal fragments RP11-918B12, RP11-916D13, RP11-416B14 and RP11-344N17. The probe set is capable of diagnosing the specificity and the sensibility of Xp11.2 ectopic PEComa 100%, is convenient, quick and reliable, and can ensure a high success rate. And moreover, the probe set can be used for preparing an Xp11.2 ectopic PEComa diagnostic kit.

The invention discloses a cell line for lowly-metastatic clear cell carcinoma of kidney of Han Chinese, namely LoMeT-ccRcc CCTCC No.C200910. The cell line can grow for a long time in vitro and be stably handed down, has epithelioid cell form, and has no contact inhibition. The cell is heteroploid, chromosome number and structure are aberrant. Immunohistochemistry shows masculine CA9 and CD133. A flow cytometry analyses and finds out that CA9 and CD133 expression is obviously enhanced in the condition that cell is handed down for many times. The cell line has lowly-metastatic trend after the cell is handed down continuously. The recovery rate of the cell line after being frozen is more than 80%, the recovered cell growth state accords with the original cell. The cell line of the invention is used for establishing a cell model for sieving and preparing medicines that is used for dialogising, preventing and treating clear cell carcinoma of kidney.

The invention provides a Jian carp brain cell line and its application. The Jian carp brain cells are obtained from the primary separation of the Jian carp brain tissue by using a suitable concentration of trypsin and setting reasonable digestion conditions. The Jian carp brain cell line CCB‑J was established, which mainly manifests as epithelial-like cells and can be stably passed down for more than 60 generations. The field has high research and application value, such as: application in virus identification and strain isolation, application in preparation of vaccines for prevention or treatment of carp virus, as a tool for drug screening, drug preparation, and drug evaluation. Applications in biological models, etc.

The invention relates to a method for building a rat islet epithelioid stem cell line, and belongs to the field of animal cell (tissue) engineering. Collagenase digests pancreatic tissue of an adult rat, pancreas islet is manually chosen, RPMI1640 is cultivated by serum, rat islet primary epithelioid cells are adhered to the wall to grow, a cloning ring is screened, the epithelioid cells are purified, and trypsin digestive juice digests and goes down to the future generation, so as to build the cell line. The rat islet epithelioid stem cell line built by adopting the method has the characteristics that the cell attachment forms polygon epithelioid, is in cloned growth, and is a normal diploid cell. An octamer transcription factor 4, a paired gene cassette 4, a paired gene cassette 6, a neurogenic factor 3 and a nidogen are expressed at the protein level, and the rat islet epithelioid stem cell line has multi-differentiating potential and is free of tumorigenicity.

The invention provides a method for inducing human epidermal stem cells into corneal epithelioid cell differentiation in vitro. A specific cornea promoting peptide is used, so that the differentiationefficiency of the cornea epithelial cells can be obviously improved.