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High metastasis human breast carcinoma cell subline and establishing method thereof

A technique for establishing methods for human breast cancer, which is applied in the field of cancer cell lines, can solve the problems of high experimental investment and long experimental cycle of human breast cancer cell line MDA-MB-435, and achieve the effect of improving the success rate

Inactive Publication Date: 2011-05-25
SHANGHAI INST OF ONCOLOGY
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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a kind of highly metastatic human breast cancer cell line, to solve many deficiencies existing in the prior art, such as human breast cancer cell line MDA-MB-435 experiment period is longer and experiment investment higher defect

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  • High metastasis human breast carcinoma cell subline and establishing method thereof
  • High metastasis human breast carcinoma cell subline and establishing method thereof
  • High metastasis human breast carcinoma cell subline and establishing method thereof

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Embodiment Construction

[0036] In order to make the technical means, creation features, achievement goals and effects of the present invention easy to understand and understand, the present invention will be further described below with reference to the specific drawings.

[0037] 1. Materials:

[0038] 1. Tumor cells

[0039] Human breast cancer highly metastatic cell MDA-MB-231 was donated by Hutchison Whampoa (Shanghai) Pharmaceutical Co., Ltd. (originally from ATCC American Type Culture Collection No. ATCC HTB-26 TM ), the subcutaneous transplanted tumor MDA-MB-231 was preserved and passaged by Shanghai Cancer Institute.

[0040] 2. Cell Culture Reagents

[0041] Dulbecco MEM (DMEM) medium was purchased from SIGMA-ALDRICH company; fetal bovine serum was purchased from PAA laboratories GmbH; trypsin was purchased from GIBCO BRL company; physiological saline, gentamicin, 10% neutral formaldehyde solution, PBS buffer All of them are imported subpackaged or domestic analytical reagents.

[0042...

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Abstract

The invention relates to a high metastasis human breast carcinoma cell subline MDA-MB-231sci, which has biological and pathological features and special metastasis phenotypes. The biological features are as follows: the MDA-MB-231sci cells are typical polygonal epithelioid cells, have rich cytoplasm and big and round karyon, grow adherent to the wall with almost uniform size and are closely arranged with clear boundary. The pathological features are as follows: a tumor cell of a nude mouse in vivo metastasis part has the similar structure with the subcutaneous tumor cell, short metastasis time and high metastasis rate, wherein the metastasis time is shortened to 26-42 days; a lung metastasis rate is 100%, meanwhile, a part of axillary lymph node metastasis of an animal is performed. An establishing method for the high metastasis human breast carcinoma cell line comprises the following steps of: (1) establishing a human breast carcinoma orthotopic transplantation tumor model; (2) sieving an orthotopic transplantation lung targeted metastasis model; and (3) establishing and passaging a human breast and lung carcinomas targeted high metastasis cell line. In the invention, the success rate is improved by sieving surgical excision tumor of each generation in vivo and corresponding breast.

Description

technical field [0001] The invention relates to a cancer cell line, in particular to a human breast cancer highly metastatic cell subline and a method for establishing the same, and belongs to the technical field of animal cell lines. Background technique [0002] Tumor invasion and metastasis is one of the most basic biological characteristics of malignant tumors. Primary tumor death is relatively rare. More than 90% of tumor patients die when tumor cells metastasize in the human body. [0003] Breast cancer is one of the main diseases that endanger women's physical and mental health, and it has become the most common malignant tumor in women. In the United States, 45,000 breast cancer patients die each year due to distant metastasis. [0004] However, the exact mechanism of tumor metastasis is still unclear, and tumor metastasis has always been a hot spot in oncology research. MDA-MB-435 is a commonly used human breast cancer cell line in the research on breast cancer in...

Claims

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Application Information

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IPC IPC(8): C12N5/09A01K67/027C12R1/91
Inventor 闫明霞姚明刘蕾赵方瑜朱淼鑫王晓敏孔韩卫
Owner SHANGHAI INST OF ONCOLOGY
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