PCR primer for detecting duck adenovirus type 4 as well as detection method and application of PCR primer
An adenovirus and primer amplification technology, applied in the field of molecular biology, to achieve strong specificity, rapid detection, and high sensitivity
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[0046] In order to make the object, technical solution and technical effect of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments. It should be understood that the specific implementations described in this specification are only for explaining the present invention, not for limiting the present invention.
[0047] The experimental materials and reagents used, unless otherwise specified, are conventional consumables and reagents that can be obtained from commercial channels.
[0048] Primer design
[0049] Primers were designed according to the duck adenovirus type 4 Hexon gene sequence. After a large number of comparisons and screenings, primers F and R with high sensitivity and specificity were selected, and their base sequences are as follows:
[0050] Primer F: 5'-GGGCAAATGACCAACCTCTA-3' (SEQ ID NO.1);
[0051] Primer R: 5'-TGTCCCTGAACCCGATGTAA-3' (SEQ ID NO. 2).
[0052] Establishment ...
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