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PCR primer for detecting duck adenovirus type 4 as well as detection method and application of PCR primer

An adenovirus and primer amplification technology, applied in the field of molecular biology, to achieve strong specificity, rapid detection, and high sensitivity

Pending Publication Date: 2020-12-08
INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Based on the researcher's discovery that the positive rate of duck adenovirus type 4 has reached about 15%, it is likely to continue to rise, therefore, an effective PCR primer (amplification primer) that can be used to detect duck adenovirus type 4 is provided, and established A set of rapid detection methods and systems are of great clinical significance

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  • PCR primer for detecting duck adenovirus type 4 as well as detection method and application of PCR primer
  • PCR primer for detecting duck adenovirus type 4 as well as detection method and application of PCR primer
  • PCR primer for detecting duck adenovirus type 4 as well as detection method and application of PCR primer

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Embodiment Construction

[0046] In order to make the object, technical solution and technical effect of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments. It should be understood that the specific implementations described in this specification are only for explaining the present invention, not for limiting the present invention.

[0047] The experimental materials and reagents used, unless otherwise specified, are conventional consumables and reagents that can be obtained from commercial channels.

[0048] Primer design

[0049] Primers were designed according to the duck adenovirus type 4 Hexon gene sequence. After a large number of comparisons and screenings, primers F and R with high sensitivity and specificity were selected, and their base sequences are as follows:

[0050] Primer F: 5'-GGGCAAATGACCAACCTCTA-3' (SEQ ID NO.1);

[0051] Primer R: 5'-TGTCCCTGAACCCGATGTAA-3' (SEQ ID NO. 2).

[0052] Establishment ...

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Abstract

The invention discloses a PCR primer for detecting duck adenovirus type 4 as well as a detection method and application thereof. The primer capable of rapidly detecting the duck adenovirus type 4 andthe PCR detection method of the primer are established for the first time, operation is easy, cost is low, and consumed time is short. Moreover, the PCR detection method for detecting the duck adenovirus type 4 is high in accuracy, good in specificity and high in sensitivity, the lowest detection limit is 6*10 <1> copies, clinical popularization and application are facilitated, and technical support is provided for carrying out adenovirus type 4 molecular epidemic virology investigation and related detection work in duck flocks.

Description

technical field [0001] The invention relates to the field of molecular biology, and relates to a PCR primer for detecting duck adenovirus type 4, a detection method and an application thereof. Background technique [0002] In recent years, with the rapid development of the waterfowl industry, the adenovirus infection in ducks is increasing, and the main feature of duck adenovirus infection is inclusion body hepatitis. According to the investigation, duck adenovirus is divided into four genotypes, including duck adenovirus type 1 (duckadenovirus 1, DAdV-1), duck adenovirus type 2 (duck adenovirus 2, DAdV-2), duck adenovirus type 3 (duckadenovirus 3, DAdV-3) and duck adenovirus type 4 (duck adenovirus 4, DAdV-4). Duck adenovirus type 4 was first discovered in 2019. It was isolated by researchers in the laboratory from clinical egg-laying shelduck ducks suffering from salpingitis. According to the evolutionary tree analysis, it was found that the new adenovirus belongs to the ...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/686C12N15/11C12R1/93
CPCC12Q1/701C12Q1/686C12Q2565/125
Inventor 张俊勤黄允真董嘉文李林林孙敏华张建峰
Owner INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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