Metagenome-based human adenovirus molecular typing and tracing method and system

Abstract

The invention discloses a metagenome-based human adenovirus molecular typing and tracing method and system, and the method comprises the steps: constructing a human adenovirus typing database comprising a typing hierarchical classification system, and obtaining a classification unit and a complete genome / marker gene sequence of human adenoviruses, determining a complete genome / marker gene sequence of each classification unit of the human adenovirus, obtaining metagenome sequencing original data of a clinical sample, preprocessing the metagenome sequencing original data to obtain target metagenome data, analyzing the target metagenome data by utilizing a preset metagenome sequencing data dual-comparison annotation system, determining whether the clinical sample has a target human adenovirus classification unit or not, and when it is determined that the clinical sample has the target human adenovirus classification unit, determining a target associated feature corresponding to the target human adenovirus classification unit, and generating a human adenovirus typing identification report. Classification unit (subtype / genotype) typing identification of human adenoviruses can be carried out on clinical infection samples with low virus content.

Description

technical field [0001] The invention relates to the technical field of biological information, in particular to a method and system for molecular typing and traceability of human adenovirus based on metagenomics. Background technique [0002] Human adenovirus (HAdV) belongs to the family Adenoviridae (Adenoviridae) mammalian adenovirus (Mastadenovirus), the genome length is about 34.7kb, belongs to dsDNA, and can infect a variety of mucosal tissues, such as gastrointestinal tract, respiratory tract, urinary tract, etc. Genital tract and cornucopia, etc., leading to self-limiting mucosal infection or even severe fatal infection. There are three main envelope proteins on the surface of HAdV, which are important components of the envelope and important antigens for diagnosis. In genetics, the regions encoding these three types of proteins are the most variable regions in the virus genome, and are the hotspots of human adenovirus gene research. According to immunological, biol...

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Problems solved by technology

[0004] The present invention proposes a metagenomic-based human adenovirus molecular typing and traceability method and system to solve the current metagenomic sequencing typing of human adenovirus mentioned in the background technology requires long sequencing reads and virus in the sample The virus content is high, and the virus is annotated at the species level through assembly and comparison strategies, and genotype level typing identification cannot be provided, so it is impossible to perform more comprehensive human adenovirus typing identification on clinical samples with low virus content question

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