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Preparation method and application of phenylpropanoid compound in tobacco

A compound, the technology of phenylpropanoid, applied in the field of tobacco chemistry, has achieved good application prospects, simple structure and good activity

Active Publication Date: 2015-09-30
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention has isolated a phenylpropanoid compound with anti-rotavirus activity from tobacco, which has not been reported so far

Method used

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  • Preparation method and application of phenylpropanoid compound in tobacco
  • Preparation method and application of phenylpropanoid compound in tobacco
  • Preparation method and application of phenylpropanoid compound in tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of phenylpropanoid compound C 11 h 10 o 5 , including extracting extract, silica gel column chromatography, and high-pressure liquid chromatography separation, specifically adopting the following steps:

[0029] Step (1), extraction of extract: take tobacco leaves and crush them, use high-concentration methanol (w%: 95%) or high-concentration ethanol (w%: 95%) or high-concentration acetone (w%: 70%) as the extraction solvent, Extraction solvent: Tobacco (weight ratio) = 2:1, soak for 54 h, extract 4 times, combine extracts, filter and concentrate to obtain extract.

[0030] Step (2), silica gel column chromatography: after dissolving the extract obtained in step (1) with 2.5 times the weight of pure methanol, pure ethanol or pure acetone, use 80-100 mesh silica gel with 1.2 times the weight of the extract Mix the sample, and at the same time, dry-pack the column with 250-mesh silica gel that is 3 times the weight of the extract, and then dry-load the samp...

Embodiment 2

[0035] The tobacco samples were from Yuxi, Yunnan, and the variety was Yuxi K326. Sampling 2.0 kg of tobacco and pulverizing it with 95% methanol soaking and extracting 5 times, extracting for 24 h each time, extracting solvent: weight ratio of tobacco leaves = 2:1, combining the extracts, filtering, concentrating under reduced pressure to obtain extract 105 g. After dissolving the extract with 2.0 times its weight of pure methanol, mix the sample with 120 g of 100-mesh silica gel, pack 0.6 kg of 160-mesh silica gel into a column, and perform silica gel column chromatography with a volume ratio of 1:0. , 20:1, 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring The same parts were combined and concentrated to obtain 8 parts, Wherein the chloroform-acetone elution and concentration with a volume ratio of 6:4 is used to separate the part obtained by Agilent 1100 semi-preparative high-performance liquid chromatography, with 28% methanol as the mobile...

Embodiment 3

[0038] Tobacco samples are from Dali, Yunnan, and the variety is Yunyan 200. 3.5 kg of tobacco samples were cut into small pieces, soaked and extracted with 95% ethanol for 4 times, each time for 48 h, the weight ratio of extraction solvent:tobacco leaves = 4:1, and the extraction The liquids were combined, filtered, concentrated under reduced pressure to form an extract, and 250 g of extract was obtained. After the extract is dissolved in pure methanol whose weight is 2.0 times of its weight, the sample is mixed with 250 g of 80 mesh thick silica gel, and 0.8 kg of 200 mesh silica gel is packed into a column for silica gel column chromatography, and the volume ratio is 1:0, 20:1, 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring The same parts were combined and concentrated to obtain 8 parts, of which The part obtained by eluting and concentrating with chloroform-acetone with a volume ratio of 6:4 was separated by Agilent 1100 semi-preparative h...

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Abstract

The invention discloses a of a phenylpropanoid compound showing as the first formula (Please see the formula in the instruction) and a preparation method and application thereof and belongs to the technical field of tobacco chemistry. Tobacco serves as a raw material, extraction is conducted with a high-concentration methanol aqueous solution or a high-concentration ethanol aqueous solution or a high-concentration acetone aqueous solution as an extraction solvent, and the extraction solutions are merged, filtered, and decompressed condensed to form extract; the extract is packed by the adoption of a silica gel to be conducted silica gel column chromatography; a mixing solvent of chloroform-acetone serves as an eluent to conduct gradient elution, and TLC monitors and merges same parts and condenses; separation and purification by use of high-pressure liquid chromatography are further conducted on the part obtained by eluting, collecting and condensing by the mixing solvent of the chloroform-acetone with a volume matching of 6:4, and the phenylpropanoid compound is obtained. The activity test indicates that the compound has a good inhibiting effect on rotavirus. The compound is simple in structure, good in activity and capable of being used as a guiding compound for resisting the rotavirus.

Description

technical field [0001] The invention belongs to the technical field of tobacco chemistry, in particular to a phenylpropanoid compound extracted from tobacco for the first time. At the same time, the invention also relates to the preparation method and application of the compound. Background technique [0002] Tobacco is the plant with the most complex chemical composition in the world, and its secondary metabolites are very rich. After decades of research, more than 3,000 monomeric chemical substances have been identified from tobacco, and many components have not yet been identified. come out. In addition to being mainly used for smoking cigarettes, tobacco can also extract a variety of valuable chemical components from it, and discover leading compounds that are valuable for development and utilization. [0003] Phenylpropanoids are a class of biologically active substances that widely exist in nature. Because plant phenylpropanoids have many types of structures, comple...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/88A61P31/14
CPCC07D307/88
Inventor 刘春波缪明明申钦鹏杨光宇张凤梅何沛司晓喜苏钟璧刘志华陈永宽朱瑞芝王昆淼
Owner CHINA TOBACCO YUNNAN IND
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