200 results about "Surface plasmon resonance sensor" patented technology

This invention provides methods, devices and device components for sensing, imaging and characterizing changes in the composition of a probe region. More particularly, the present invention provides methods and devices for detecting changes in the refractive index of a probe region positioned adjacent to a sensing surface, preferably a sensing surface comprising a thin conducting film supporting surface plasmon formation. In addition, the present invention provides methods and device for generating surface plasmons in a probe region and characterizing the composition of the probe region by generating one or more surface plasmon resonances curves and / or surface plasmon resonance images of the probe region.

A sensor chip has a metal layer formed on a surface of a substrate, where a plurality of microscopic concave part is formed in a measurement region of the surface of the metal layer. When light of linear polarization is irradiated onto the measurement region, local resonance electric field generates at opposing metal layer surfaces in the concave part. The reflected light thereof is received to measure reflectance. The light of linear polarization is irradiated so that the polarizing surface becomes orthogonal to the longitudinal direction of the concave part.

There is provided a surface plasmon resonance sensor including a metallic graphene layer formed in a core of a predetermined area of an optical fiber from which a jacket and a cladding are removed. Further, a preparing method of the surface plasmon resonance sensor and a surface plasmon resonance sensor system is also provided.

An objective of the present invention is to provide a measuring chip for a surface plasmon resonance sensor that can detect a small amount of target substances in high sensitivity. The present invention provides a measuring chip for a surface plasmon resonance sensor comprising a metal layer, one or more plasma polymerization layers formed on said metal layer, and a biologically active substance immobilized on the surface of said plasma polymerization layer.

A fiber-optic surface plasmon resonance sensor may include an optical fiber and a surface plasmon excitation layer. The optical fiber may include a core, a cladding surrounding the core, and a depression. The surface plasmon excitation layer may include a first excitation layer, a second excitation layer and an optical waveguide layer between the first excitation layer and the second excitation layer. Incident light incident through the core may be coupled to the surface plasmon excitation layer at a specific angle of incidence and wavelength satisfying the surface plasmon resonance condition. Depending on the polarizing direction of the incident light, an s-polarized component may be coupled to the guided-wave mode in the optical waveguide layer constituting the surface plasmon excitation layer.

A sensor for detecting the presence of a target analyte, ligand or molecule in a test fluid, comprises a light transmissive substrate, an array of surface plasmon resonant (SPR) elements mounted thereon, a light source arranged to direct light into the SPR elements, and a detector arranged to detect light transmitted through the SPR elements. The SPR elements may include a dielectric polystyrene sphere having a spherical diameter of about 770 nm with a gold coating layer about 150 nm thick. The interface between the SPR elements and the substrate forms a pinhole with a diameter less than the wavelength of the light directed into the elements to produce a near-field coupling mode within the micro-sized SPR elements. In specific embodiments, the pinhole diameter ranges between 150-200 nm. The SPR elements are contained within a molded PDMS chip that may incorporate micro-fluidic components, such as a pump and valves, to control flow of the test fluid across the SPR elements. A multi-channel sensor may be provided for detecting the presence of several targets with a single micro-chip sensor. In the multi-channel sensor collections of SPR elements are commonly functionalized to one of the several targets. The detectors sense changes in the resonant response of the SPR elements indicative of binding with the targets.

Disclosed is apparatus and method for controlled surface plasmon resonance analysis having a surface plasmon resonance sensor (200) with a derivatized surface plasmon layer (116) in optical communication with the sensor, derivatizing the surface plasmon layer and placing an analyte detection chamber (102) in fluid communication with the derivatized surface plasmon layer. The chamber is adapted (118, 120) for the generation of a molecular interaction bias across the chamber. A conjugate is provided between an analyte and a bias responsive element, wherein the analyte is reactive with the derivatized surface plasmon layer and the bias responsive element changes the response of the analyte to the molecular interaction bias. A conjugated analyte may be introduced into the chamber, generating a molecular interaction.

An SPR sensor comprising: a thin conducting layer comprising at least one conductive element formed on a surface of a transparent substrate; an illumination system controllable to illuminate an interface between the conducting layer and the substrate; a photosensitive surface that generates signals responsive to light from the light source that is reflected from a region of the interface; a flow cell formed with at least one flow channel having a lumen defined by a wall at least a portion of which is formed from an elastic material and a portion of which is formed by a region of the conducting layer; and at least one hollow needle having an exit orifice communicating with the needle's lumen and wherein fluid flow is enabled between the flow channel and the needle's lumen by puncturing the elastic material with the at least one needle so that the exit orifice communicates with the flow channel lumen.

An SPR sensor comprising: a thin conducting layer comprising at least one conductive element formed on a surface of a transparent substrate; an illumination system controllable to illuminate an interface between the conducting layer and the substrate; a photosensitive surface that generates signals responsive to light from the light source that is reflected from a region of the interface; a flow cell formed with at least one flow channel having a lumen defined by a wall at least a portion of which is formed from an elastic material and a portion of which is formed by a region of the conducting layer; and at least one hollow needle having an exit orifice communicating with the needle's lumen and wherein fluid flow is enabled between the flow channel and the needle's lumen by puncturing the elastic material with the at least one needle so that the exit orifice communicates with the flow channel lumen.

The invention relates to a surface plasma resonance sensor chip and a preparation method thereof, in particular to a photografting molecularly imprinted polymer membrane-based surface plasma resonance sensor chip and a preparation method thereof, and belongs to the field of surface plasma sensor chip preparation. The chip comprises a glass substrate, a gold membrane layer, a unimolecular alkyl chain layer and a molecular imprinted polymer membrane, wherein the gold membrane layer is plated on the glass substrate, the unimolecular alkyl chain layer is self-assembled on the glass substrate, and the molecular imprinted polymer membrane is grafted on the unimolecular alkyl chain layer in turn. The molecular imprinted membrane with nanoscale thickness can be conveniently and quickly prepared on the surface of the gold membrane by the preparation method and has controllable thickness; the growth changes of the molecular imprinted membrane can be dynamically monitored in real time in situ; operation steps are simple and deoxidation and nitrogen protection are not needed; and the matrix type molecular imprinted structural units are easy to prepare on the surface of the gold membrane, each unit can be different template molecules and multi-channel and multi-target molecular detection is realized.

An objective of the present invention is to provide a measuring chip for a surface plasmon resonance sensor that can detect a small amount of target substances in high sensitivity. The present invention provides a measuring chip for a surface plasmon resonance sensor comprising a metal layer, one or more plasma polymerization layers formed on said metal layer, and a biologically active substance immobilized on the surface of said plasma polymerization layer.

Disclosed is apparatus and method for controlled surface plasmon resonance analysis having a surface plasmon resonance sensor (200) with a derivatized surface plasmon layer (116) in optical communication with the sensor, derivatizing the surface plasmon layer and placing an analyte detection chamber (102) in fluid communication with the derivatized surface plasmon layer. The chamber is adapted (118, 120) for the generation of a molecular interaction bias across the chamber. A conjugate is provided between an analyte and a bias responsive element, wherein the analyte is reactive with the derivatized surface plasmon layer and the bias responsive element changes the response of the analyte to the molecular interaction bias. A conjugated analyte may be introduced into the chamber, generating a molecular interaction.

Wearable or implantable devices combining microfluidic control of sample and reagent flow and micro-cavity surface plasmon resonance sensors functionalized with surface treatments or coatings capable of specifically binding to target analytes, ligands, or molecules in a bodily fluid are provided. The devices can be used to determine the presence and concentration of target analytes in the bodily fluids and thereby help diagnose, monitor or detect changes in disease conditions.

There is provided a surface plasmon resonance sensor including a metallic graphene layer formed in a core of a predetermined area of an optical fiber from which a jacket and a cladding are removed. Further, a preparing method of the surface plasmon resonance sensor and a surface plasmon resonance sensor system is also provided.

A high-sensitivity SPR (surface plasmon resonance) sensor includes at least a prism having a first surface on which a metallic layer and a metallic nanoparticle layer are sequentially formed. A light source projects an incident light into the prism through a second surface of the prism. The light is reflected by the metallic layer and the metallic nanoparticle layer and leaves the prism through a third surface of the prism. A light detector detects the reflected light. The SPR sensor has an extensive detection range as compared with the conventional ones and is applicable in the detection of gas, chemical substance, and biomolecule. Moreover, the SPR sensor is advantageous in arranging fabrication process consistently, controlling film thickness, improving product quality, and decreasing fabrication cost.

Disclosed is an optical sensing device including a source unit providing a beam of light with continuously modulated phase retardation between p- and s-polarization components of the light by employing a LCM; a reference unit receiving a first part of the light to provide a reference signal; a SPR sensing unit receiving a second part of the light to induce a phase retardation change between the p- and s-polarization components due to SPR associated with a sample; a probe unit receiving the light after SPR to provide a probe signal; and a detection unit connected to the reference unit and the probe unit to detect characteristics of the sample by comparing the reference signal with the probe signal. By using active phase modulation technologies and differential phase measurement, it is possible to fulfill chemical and biological detection.

The invention discloses a micro-structured optical fiber surface plasmon resonance sensor, wherein a cladding layer is an annular cladding layer consisting of three concentric layers; the inner annular cladding layer consists of fan-shaped annular supporting arms (3) and inner layer air holes (2); the outer annular cladding layer consists of rectangular supporting arms (6) and outer layer liquid holes (5); the middle annular cladding layer is connected with the fan-shaped annular supporting arms (3) and the rectangular supporting arms (6); the number of both the fan-shaped annular supporting arms (3) and the rectangular supporting arms (6) is N; and the sensor has 360 / No rotational symmetry. The fan-shaped annular supporting arms are used for adjusting coupling depth; and the spatial distribution of a fiber core fundamental mode is enabled to be gaussian. The operation wavelength of the sensor is at C+L wavelength; the spectral sensitivity within an analytic liquid refractive index range of 1.320-1.335 can reach over 10,000 nm RIU<1>, and the corresponding sensing resolution reaches the magnitude of 10<5> RIU; and the micro-structured optical fiber surface plasmon resonance sensor is suitable for high-accuracy real-time detection of liquid refractive index.

In a surface plasmon resonance sensor, a sensor unit includes a dielectric block, a thin film layer which is formed on the upper surface of the dielectric block, and a sample holding portion. An incubator is provided, the surface plasmon resonance sensor is spatially isolated from the surroundings by placing it in the measuring system, the temperature of the measuring system is measured and temperature change of the sensor unit after it is conveyed to the measuring system from the incubator is estimated on the basis of the temperature difference between the temperature of the incubator and the temperature of the measuring system, and the sensor unit is conveyed to the measuring system to perform the measurement within a time for which the temperature of the sensor unit does not unacceptably change.

Present invention discloses a method utilizes nano gold particle catalytic rising to raise surface plasma resonance transducer sensitivity. It coats 20-35 nm silicon dioxide thin layer on plasma resonance transducer metallic film surface, utilizes nano gold particle catalyzing to enhance surface plasma resonance signal and to raise plasma SPR transducer sensitivity. Said invention makes nano gold particle catalyzing rising in surface plasma resonance SPR transducer, in nano gold particle catalyzing process, gold or silver ion depositing on nano gold particle surface by catalytic reduction not depositing on metallic film surface to result in background heightening. Said method has simple, easy operation and high sensitivity.

Provided is a surface plasmon resonance sensor including: a part of delivering light by which a signal beam is incident to generate an evanescent field; and a part of exciting surface plasmon for exciting surface plasmons by the generated evanescent field and giving rise to a surface plasmon resonance, wherein a dielectric waveguide layer is inserted between metal layers of the part of exciting surface plasmon, and surface plasmon resonance properties are changed by an object to be analyzed.

The invention provides construction of a surface plasma resonance sensor based on the amplification effect of a magnetic molecularly imprinted polymer and the application of the surface plasma resonance sensor in pesticide detection, and belongs to the technical field of surface plasma resonance sensing. Chlorpyrifos is regarded as a template molecule firstly; the magnetic molecularly imprinted polymer which has recognition action on the chlorpyrifos is compounded by adopting the self-polymerization performance of dopamine; after elution is performed on the chlorpyrifos, the magnetic molecularly imprinted polymer containing imprinted cavities has good superparamagnetism and high selectivity; the specific interaction between acetylcholinesterase fixed on the surface of a gold sheet and the chlorpyrifos is adopted to construct a pesticide residue recognition, detection and sensing interface on the basis of the amplification effect of the magnetic molecularly imprinted polymer. Compared with the conventional pesticide residue detection technology, the surface plasma resonance sensor on the basis of the amplification effect of the magnetic molecularly imprinted polymer has high detection sensitivity and good selectivity to chlorpyrifos detection, and has an excellent application prospect.

A metal layer 13 made of Au or the like is formed on the upper surface of a transparent substrate 12. Dielectric layers 14a, 14b and 14c with different thicknesses are formed on the upper surface of the metal layer 13 (any one of the dielectric layers can have a thickness of 0) to form respective determination areas 15a, 15b and 15c. Further, different types of antibodies 22a, 22b and 22c are fixed on the upper surfaces of the respective dielectric layers 14a, 14b and 14c. Then, light is directed to the determination areas 15a, 15b and 15c, then signals of light reflected by the determination areas 15a, 15b and 15c are received, the light is dispersed, and analyses are performed on signals resulted from the light dispersion to detect the conditions of the surfaces of the respective determination areas, at the same time.

A surface plasmon resonance sensor comprising a chip having a substrate (102) and a metal layer (103), a prism (104), an optical system (105) becoming a light source, and a photodetector (106), wherein the metal layer (103) consists of a flat part (109) in the form of a thin film, and protrusions consisting of fine metal particles (110) or the like arranged at intervals. When light impinges on such a metal layer (103), a resonance angle caused by the flat part (109) and each protrusion is attained. Variation in the refractive index of a medium touching the metal layer can be detected from the resonance angle.

This invention provides methods, devices and device components for sensing, imaging and characterizing changes in the composition of a probe region. More particularly, the present invention provides methods and devices for detecting changes in the refractive index of a probe region positioned adjacent to a sensing surface, preferably a sensing surface comprising a thin conducting film supporting surface plasmon formation. In addition, the present invention provides methods and device for generating surface plasmons in a probe region and characterizing the composition of the probe region by generating one or more surface plasmon resonances curves and / or surface plasmon resonance images of the probe region.

The invention provides an inner wall-silver plated and liquid crystal-filled hollow optical fiber surface plasmon resonance temperature sensor and belongs to the optical fiber sensing technical field. According to the structure of the sensor, the interior of a hollow glass optical fiber is plated with silver, and the silver-plated hollow glass optical fiber is filled with liquid crystal; the inner diameter of the hollow glass optical fiber ranges from 300 microns to 500 microns, the numerical aperture of the hollow glass optical fiber is not lower than 0.27, and the length of the hollow glass optical fiber ranges from 30 mm to 50 mm; sensitization treatment is performed on the inner wall of the hollow glass optical fiber, and a 45nm-to 80nm uniform silver film is formed in the optical fiber through adopting a liquid phase chemical deposition method, and therefore, a sensing layer of surface plasmon resonance can be formed; and the hollow optical fiber is filled with thermotropic nematic liquid crystal, and plastic-clad multimode optical fibers are coupled to the ports of the optical fiber respectively, and end sealing processing is realized. With the inner wall-silver plated and liquid crystal-filled hollow optical fiber surface plasmon resonance temperature sensor adopted, subtle temperature changes can be dynamically monitored in real time. The inner wall-silver plated and liquid crystal-filled hollow optical fiber surface plasmon resonance temperature sensor is suitable for long-distance transmission. According to the inner wall-silver plated and liquid crystal-filled hollow optical fiber surface plasmon resonance temperature sensor, special inner wall silver plating technology is adopted, and the hollow optical fiber is filled with the thermotropic liquid crystal, and therefore, the oxidation of the external silver layer of the solid optical fiber surface plasma resonance sensor can be avoided.

Disclosed is a horizontal SPR sensor apparatus that provides a simplified beam path, ease of sample handling with concomitant ease of analysis. The horizontal SPR device disclosed herein includes an input and output transfer mirror pair that translates vertically along an axis perpendicular to the beam path. The invention includes an input / output scanner mirror pair that is operatively linked to the transfer mirror pair. This allows for rotation of the scanner mirror pair in response to the vertical translation of the transfer mirror pair and maintains the incident light focus on the metal film / sample layer while varying the angle of incidence and thereby exciting the surface plasmon resonance at the thin metal film layer. Further, this allows the detector and detector optics to be fixed providing for the implementation of heavier multi-stage cooled detectors. Finally, the invention provides an SPR device having a smaller footprint than known alternatives.

The invention discloses a long-range surface plasma resonance sensor and a preparation method thereof. The sensor is formed by bonding a BK7 prism and a glass slide through cedar oil, wherein the outer surface of the glass slide is plated with a magnesium fluoride thin film, a silver film and a gold film in turn; the gold film is directly contacted with biological solution to be detected and is used for detecting the biological solution in a mode of angle modulation; magnesium fluoride with the refractive index of about 1.38, which is close to that of the biological solution, can form approximatively symmetric structures on two sides of a metal conductive layer so as to ensure that surface plasmas on the two sides of the metal conductive layer are coupled to form the long-range surface plasma; compared with other metals, the metal silver used as the conductive layer has a narrower half band width of a resonance absorption peak, but has unsteady chemical properties; a conductive layer gold film can make a resonance angle generate a greater transition along with the change of the refractive index of the biological solution to be detected; simultaneously, the conductive layer gold film has stable chemical properties and high biological affinity. The specific multilayer film structure can integrate the advantages of each film and effectively improve the sensitivity of biological sensors.

Implemented is a chip for localized surface plasmon resonance sensor, which is able to provide a localized surface plasmon resonance sensor of higher sensitivity. A structure of the invention is characterized by including a planar section and tubular bodies, wherein the tubular bodies are vertically arranged so that openings thereof open at the planar surface of the planar section, an average inner diameter of the openings of the tubular bodies is within a range of from 5 nm to 2,000 nm, a ratio (A / B) of inner diameter A of the openings of the tubular bodies and inner diameter B at the midpoint of the depth from the openings of the tubular bodies is within a range of from 1.00 to 1.80, and the bottom of the tubular bodies is aspherical.