Wavelength tunable surface plasmon resonance sensor
A surface plasmon and resonant sensor technology, which is applied in the direction of instruments, scientific instruments, measuring devices, etc., can solve the problems of method limitations, inability to provide tuned excitation or detection wavelengths, and damage the materials in the probe area, and achieve the effect of wide dynamic range
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[0096] Example 1: Characterization of an Exemplary SPR Sensor
[0097] The ability of the SPR sensor of the present invention to sense changes in the refractive index of the probe region was demonstrated by experimental and computational studies. Specifically, it is an object of the present invention to provide SPR sensors capable of sensitive detection and characterization of changes in the refractive index of the probe region. Further, it is an object of the present invention to provide an SPR sensor with a large dynamic range capable of detecting materials with a wide range of refractive indices.
[0098] To achieve the aforementioned goals, the detection sensitivity and dynamic range of an exemplary SPR sensor were modeled in silico and estimated by monitoring the refractive index of a low-concentration sucrose solution. Exemplary SPR sensors employed in these studies are based on the Kretschmann configuration and as Figure 5 shown. The polychromatic light source was...
example 2
[0105] Example 2: SPR images of thiol maps and bovine serum albumin on a gold surface
[0106] To estimate the sensitivity and spatial resolution of the inventive SPR imaging device, SPR images of the thiol map were generated by an exemplary SPR sensor. Thiol maps on gold surfaces (comprising ~1 nm of Cr and ~45 nm of gold e-beam deposited from Fisher Scientific onto standard microscope slides) were prepared using the polydimethylsiloxane (PDMS) stamping protocol . The employed protocol was optimized to minimize transfer of material from the PDMS imprint to the surface and to produce a monolayer of thiols on the surface. All images were acquired with p-polarized light. Figure 12 shows a series of images of thiol and water maps acquired with optical interference filters at various tilt angles. Figure 12A corresponds to a central wavelength of 857 nm, Figure 12B corresponds to a central wavelength of 852 nm, Figure 12C corresponds to a central wavelength of 845 nm, Figure 1...
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