The invention discloses a laser speckle blood flow imaging contrast analytical method. The laser speckle blood flow imaging contrast analytical method comprises following steps that a laser beam illuminates a measured object, rear scattered light of the laser beam is collected by an imaging system and forms an image on an image sensor, after a current original speckle image of the measured objectis divided by a mean value of a previous N-frame original speckle image on a time axis, so that a new speckle image is obtained, an Ns*Ns spatial statistic window analyzes spatial statistical property of the new speckle image so that a contrast image Kns is obtained, and a corresponding biological tissue two-dimensional blood flow distribution graph is obtained via the relation of contrast and blood flow speed. The laser speckle blood flow imaging contrast analytical method realizes accurate assess of local blood flow change, blood flow imaging and blood kinetic parameters of biological tissues in an endoscopic monitoring cavity at a high time resolution and low noise level, can be widely applied to dynamically monitoring blood flow distribution and change of insides of abdominal cavitiesof animals, insides of joints, stomach walls and the like, focus is positioned, pathological change of diseases are assessed, a medical effect is researched, and a higher clinical application value is realized.