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Five-dimensional fluorescent microscope imaging technique

A fluorescence and functional imaging technology, applied in the field of five-dimensional fluorescence microscopic imaging technology, can solve the problems of insufficient life measurement range, insufficient time resolution, and no spectral resolution, etc., to achieve in vivo measurement, deep imaging depth, The effect of hyperspectral resolution

Inactive Publication Date: 2006-02-22
SHENZHEN UNIV
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Problems solved by technology

[0006] The invention mainly solves the problems of long measurement time, insufficient time resolution, insufficient lifetime measurement range, and no spectral resolution in the current fluorescence lifetime microscopy imaging technology, and proposes a method for simultaneously obtaining spectral information and lifetime information of organisms. , and can be three-dimensional imaging method

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  • Five-dimensional fluorescent microscope imaging technique
  • Five-dimensional fluorescent microscope imaging technique
  • Five-dimensional fluorescent microscope imaging technique

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Embodiment Construction

[0023] In Figure 1, the ultrashort pulse output from the optical path of a high repetition rate ultrashort optical pulse laser such as Ti:Sapphire mode-locked femtosecond laser passes through the pulse extractor, and the repetition frequency is reduced from 76MHz to 1MHz, and then the laser beam is processed by a beam expander. Uniform beam expansion, use a microlens array such as a self-focusing lens to discretize the uniform laser beam in two dimensions, and perform two-dimensional high-spatial resolution discretization illumination on the sample through the microscope objective lens, so that the sample is illuminated in two dimensions Discrete points emit fluorescence at the same time, and these fluorescences are focused by a microscopic objective lens to generate a two-dimensional spatially discrete fluorescent point array image (1 in the figure); then a series of parallel beams are formed by a microlens array, and after passing through a prism or grating beam splitting syst...

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Abstract

This invention relates to one method to acquire biology spectrum information and life information and to realize three-dimensional imaging. The invention is characterized by the following: getting multi-photon or single photon triggering of five-dimensional fluorescent microscope imaging information, that is three-dimensional space, one dimensional time and spectrum. This multi-parameter compound measurement and technique, which can satisfy the needs of different layers in study and can realize the flexible made three-dimensional spectrum for measurement.

Description

technical field [0001] The present invention is a method that can simultaneously acquire spectral information and life information of organisms, and can also realize three-dimensional spatial imaging, and has the advantages of high temporal and spatial resolution, high spectral resolution, short measurement time, deep imaging depth, and favorable living body measurement. , is a new type of multi-spectral resolution fluorescence microscopy functional imaging technology, which can be used in biomedical research, clinical diagnosis and drug screening and other fields. Background technique [0002] The measurement of fluorescence lifetime information and spectral information is very important for the acquisition of biological function information. It helps to reveal diverse information on biomolecular components, molecular interactions, and molecular microenvironment changes. Therefore, it has caused people's attention [1，2] . [0003] Optical functional imaging is one of the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/25
Inventor 牛憨笨屈军乐林子扬王淑岩郭宝平刘立新张济康胡涛田劲东杨勤劳张焕文
Owner SHENZHEN UNIV
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