Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Measuring chip for surface plasmon resonance biosensor and method for producing the same

a surface plasmon resonance and biosensor technology, applied in the field of surface plasmon resonance biosensors, can solve the problems of lb membrane binds poorly to a metal thin film, peels off together, and conventional methods are known to be complicated, so as to achieve the effect of improving the sensitivity of the measuring chip for the surface plasmon resonance sensor

Inactive Publication Date: 2007-06-28
KARUBE ISAO +1
View PDF9 Cites 29 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0009] The present inventors have now found that sensitivity of a measuring chip for a surface plasmon resonance sensor is extremely improved when only a small amount of a physiologically active substance is immobilized on a specific plasma polymerization layer.
[0010] An objective of the present invention is to provide a measuring chip for a surface plasmon resonance sensor that can detect a small amount of target substances in high sensitivity.
[0011] Another objective of the present invention is to provide a measuring cell for a surface plasmon resonance sensor that can detect a small amount of target substances in high sensitivity.

Problems solved by technology

Conventional methods are known to be intricate and require labeling substances.
However, this method has a disadvantage in that LB membrane binds poorly to a metal thin-film and peels off together with the physiologically active substance.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Measuring chip for surface plasmon resonance biosensor and method for producing the same
  • Measuring chip for surface plasmon resonance biosensor and method for producing the same
  • Measuring chip for surface plasmon resonance biosensor and method for producing the same

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0119] A measuring chip having layers shown in FIG. 1 on an optical recognition part was constructed.

[0120] A glass plate with a thickness of 0.15 mm (18 mm×18 mm) was used for a transparent substrate. A chrome layer and then a gold layer were deposited on this transparent substrate by sputtering. The sputtering was carried out at 100 W for 40 seconds for the chrome layer and at 100 W for 2 minutes and 30 seconds for the gold layer. The resulting chrome layer was 40 angstroms thick and the resulting gold layer was 500 angstroms thick.

[0121] A plasma polymerization layer was formed on the metal layers. An apparatus as shown in FIG. 7 was used for plasma polymerization. Ethanedithiol was used as a monomer material for the plasma polymerization layer to introduce a thiol group. Conditions for plasma polymerization were as follows:

[0122] Flow volume of monomer material: 15 sccm

[0123] Temperature: 15° C.

[0124] Pressure: 4.7 Pa

[0125] Discharge electric power: 20 W

[0126] Discharge f...

example 2

[0131] The same apparatus and method as in Example 1 were used.

[0132] Acetonitrile was used as a monomer material for the plasma polymerization layer. Conditions for plasma polymerization were as follows:

[0133] Flow volume of monomer material: 1.5 sccm+Ar dilution 15 (sccm)

[0134] Temperature: room temperature

[0135] Pressure: 4.7 Pa

[0136] Discharge electric power: 80 W

[0137] Discharge frequency: 13.56 MHz

[0138] Duration of discharge: 15 seconds.

[0139] Under the conditions described above, a plasma polymerization layer was formed. The sensor chip was mounted on the cartridge block of the surface plasmon resonance biosensor, 5% glutaraldehyde was poured through a flow route into the measuring cell at a flow rate of 5 μl / min for 10 minutes and avidin (concentration: 20 μg / ml) was also poured at a flow rate of 5 μl / min to immobilize for 60 minutes. 10 μM biotin-labeled probe RNA were then poured at a flow rate of 1 μl / min to immobilize the probe RNA for 10 minutes. DNA (7.5×10−7 ...

example 3

[0141] The same apparatus and method as in Example 1 were used.

[0142] Conditions for plasma polymerization layer formation were the same as in Example 2.

[0143] Under the conditions described above, a plasma polymerization layer was formed.

[0144] The sensor chip was mounted on the cartridge block of the surface plasmon resonance biosensor, 5% glutaraldehyde was poured through a flow route into the measuring cell at a flow rate of 5 μl / min for 10 minutes and streptoavidin (concentration: 20 82 g / ml) was also poured at a flow rate of 5 μl / min to immobilize for 60 minutes. 10 μM biotin-labeled probe RNA was then poured at a flow rate of 1 μl / min for 10 minutes to immobilize the probe RNA. DNA (7.5×10−7 M) having a DNA sequence complementary to this probe RNA was introduced and after the reaction, a signal of about 375 RU was obtained.

Concentration ofComplementaryDNA (μM)0.000750.00750.0750.757.575RU7.518.7575375750825

[0145] It was confirmed by the XPS analysis that the resulting me...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

An objective of the present invention is to provide a measuring chip for a surface plasmon resonance sensor that can detect a small amount of target substances in high sensitivity. The present invention provides a measuring chip for a surface plasmon resonance sensor comprising a metal layer, one or more plasma polymerization layers formed on said metal layer, and a biologically active substance immobilized on the surface of said plasma polymerization layer.

Description

BACKGROUND OF THE INVENTION [0001] 1. Field of the Invention [0002] The present invention relates to a surface plasmon resonance biosensor, specifically, a measuring chip for the same and a method for producing the measurement chip. [0003] 2. Background Art [0004] A number of methods using immunological reactions are used in clinical tests for detecting target substances. Conventional methods are known to be intricate and require labeling substances. Thus, immunological sensors using a surface plasmon resonance biosensor (SPR) is being used, in which no labeling substance is required and a ligand can be detected with high sensitivity. This surface plasmon resonance biosensor is based on the phenomenon that the intensity of a monochromatic light reflected from the interface between an optically transparent substance such as glass and a metal thin-film layer is dependent on the refractive index of a sample placed on the reflecting side of the metal. Accordingly, a sample can be analyz...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): G01N33/53C12M1/34G01N33/543G01N21/27G01N21/41G01N21/77G01N33/545G01N33/547G01N33/553G01N37/00
CPCG01N21/553
Inventor NAKAMURA, RUNANAKAMURA, HIROYUKINAGATA, RYOHEIKARUBE, ISAOMUGURUMA, HITOSHI
Owner KARUBE ISAO
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com