The invention relates to a portable probing type surface
plasma resonance biosensor and a preparation method and a detection method of the portable probing type surface
plasma resonance biosensor. The sensor comprises strip
optical glass, an inner
meal reflecting film coats the bottom of the strip
optical glass, a
chromium film coats the side wall of the strip
optical glass, a
gold film coated with a biomolecular sensitive layer coats the nano
chromium film, a
collimator is arranged on an incident light path of the strip optical glass, and a polarizing film, an
optical fiber collimator and an
optical fiber spectrometer connected with the
optical fiber collimator by a multimode optical
fiber are sequentially arranged on an emergent light path. The inner
meal reflecting film coats the bottom end of the strip optical glass, the
chromium film and the
gold film sequentially coat the side wall of the top part of the strip optical glass, the biomolecular sensitive layer is formed on the surface of the
gold film, and BSA solution is used for sealing to obtain the probing type surface
plasma resonance biosensor. The portable probing type surface
plasma resonance biosensor is inserted into liquid in a
test tube for SPR measurement, the
system is simplified, and the portability of the sensor is achieved; and besides, the portable probing type surface
plasma resonance biosensor overcomes the disadvantages of the existing probing type SPR sensor, the measurement mode is simple and the portability of the instrument is realized.