35 results about "Surface plasmon resonance biosensor" patented technology

A near-field surface plasmon detector is provided. The near-field surface plasmon detector includes one or more semiconductor layers that absorb one or more surface plasmons of thin metal films in the vicinity of the semiconductor layer. The surface plasmons are excited by incoming light being emitted from a light emitting source. The metal films are also employed as electrical contacts used to capture photocurrent generated after absorption of surface plasmons by the semiconductor layers.

An assay apparatus of a surface plasmon resonance biosensor system includes a flow channel block loadable with a sensor unit of the chip type removably. A metal film has a sensing surface and a metal / dielectric interface being defined on a dielectric glass substrate. An optical assay unit applies illumination through the glass substrate to the metal / dielectric interface as conditioned for total reflection, detects the illumination reflected by the metal / dielectric interface during the interaction of the ligand and the analyte on the sensing surface. The assay apparatus includes a sensor storage for storing the sensor unit in an unused state. A used sensor receptacle receives the sensor unit in a used state after use in the assay. A transfer assembly retrieves the sensor unit for the assay from the sensor storage, sets the sensor unit on the flow channel block, and discharges the sensor unit toward the used sensor receptacle.

The invention relates to a prism-coupled SPR biosensor, which comprises an optical plastic prism, an inner metal film layer, an outer metal film layer and a biological component layer; The outer metal film layer is deposited on the film layer, the inner metal film layer and the outer metal film layer can be two kinds of the same or different metal film layers, and the sum of the thickness of the inner metal film layer and the outer metal film layer is between 20-80nm The space meets the optical requirements for exciting surface plasmon resonance, and a biological component layer is fixed on the surface of the outer metal film layer. The present invention also relates to the use of the prism-coupled SPR biosensor in preparing biochips and its use in detecting interactions between biological samples.

An integrated optical waveguide based surface plasmon resonance biosensor is formed by detecting amplitude and phase of electromagnetic waves utilizing interferometry and / or optical delay configurations.

The present invention provides a method of detecting human antibodies in a sera solution. The invention also provides a method of quantitating anti-glycolipid antibody levels in solutions. The invention provides a method of diagnosing disease states, including neurological diseases, by quantitating a subject's antibody levels.

The invention relates to a portable enhanced surface plasmon resonance biosensor and belongs to the field of heavy metals detection. The portable enhanced surface resonance biosensor as a GH-SPR (Goos-Hanchen surface plasmon resonance) sensor, comprising a waterproof module, wherein the waterproof module is connected with an external laser source through an adapter and is connected with an external control circuit through a connection port. ONP (open network provisioning) technology is combined with the GH-SPR sensor, and SPR coupling effect more significant than heavy metal ions themselves iscreated through connection or disconnection of DNA strands and additional AuNPs to the surface of the sensor. The sensor is simple in structure and easy to use; a DNA probe method has the advantagesof low sampling quantity, zero need for marking, isolation and purification, short detection time and the like; a DNA chip is reusable so that the cost is further reduced.

A surface plasmon resonance apparatus (10) includes a prism (12); a dielectric layer (90) contacting one face (16) of the prism (12); a conducting layer (14) having a first surface contacting the dielectric layer (90) and a second surface to contact a sample (22) to be analyzed by the surface plasmon resonance apparatus (10); and a light source (18) emitting an incident light beam (26) having a wavelength 0 entering the prism (12) and incident on the face (16) of the prism (12) contacted by the dielectric layer (90) at an adjustable incident angle alpha relative to a normal to the face (16) of the prism (12) contacted by the dielectric layer (90). The conducting layer (14) has a thickness that enables generation of slightly asymmetric coupled surface plasmons when the incident angle alpha is equal to a resonance angle determined by the wavelength 0 of the incident light beam (26), an index of refraction (n m ) of the conducting layer, and an index of refraction (n s ) of the sample (22).

The present invention discloses a coupled waveguide-surface plasmon resonance biosensor, comprising: a grating layer formed of a transparent material, the grating layer comprising a first periodic grating structure; a waveguide layer formed on the first periodic grating structure, the refractive index of the waveguide layer being larger than the refractive index of the grating layer; a plasmon resonance layer formed on the waveguide layer, capable of being optically excited to cause a plasmon resonance wave; and a ligand layer formed on the plasmon resonance layer; capable of being bound to react with receptors of a sample to be tested.

An objective of the present invention is to provide a measuring chip for a surface plasmon resonance sensor that can detect a small amount of target substances in high sensitivity. The present invention provides a measuring chip for a surface plasmon resonance sensor comprising a metal layer, one or more plasma polymerization layers formed on said metal layer, and a biologically active substance immobilized on the surface of said plasma polymerization layer.

A method of coating an SPR biosensor specific for an analyte to reduce protein fouling, the method has the steps of providing an SPR biosensor, providing a solution of 11-mercaptoundecanol; incubating the SPR biosensor in the 11-mercaptoundecanol solution to form a self-assembling monolayer (SAM); incubating the SPR with SAM in a solution of epichlorohydrin and diglyme; next incubating the SPR in ethanolamine; preparing a solution of EDCNHS and a biocompatible polymer; incubating the SPR from ethanolamine in the EDC / NHS / polymer solution; providing a ligand specific for the analyte in a solution; incubating the polymer-coated SPR in the ligand solution to permit the ligand to react with the polymer-coated SPR; washing the ligand-coated SPR to remove unreacted ligand, thereby providing an SPR capable of reacting with the analyte. Another method replaces the solution for the SAM layer with a solution of MHA or NHS-MHA with HT, and attaches the ligand to the resulting SAM layer.

A surface plasmon resonance biosensor device and system are provided. The simplicity of SPR biosensor design allows easy integration with both QCM and electrochemistry techniques, not found in current SPR biosensor devices. In some embodiments, the surface plasmon resonance biosensor device has a dual SPR / QCM sample holder which allows simultaneous detection by both surface plasmon resonance and also quartz crystal microbalance (QCM) techniques. In additional embodiments, the surface plasmon resonance biosensor device and / or the dual SPR / QCM technique can be integrated with electrochemistry techniques by incorporate reference and counter electrodes in the SPR or SPR / QCM sample holder. Methods of using the device and system to determine whether an analyte of interest is present in a sample are also provided.

The invention discloses a multichannel parallel detection surface plasma resonance (SPR) biosensor, and preparation and detection methods thereof. A sensing unit of the sensor comprises a triangular prism and a gold thin film lattice chip prepared on an optical slide, and cross-linked molecular layers and bioprobe molecules are assembled on the surfaces of lattice points; a halogen tungsten lamp, a precision monochrometer, a spherical lens, a diaphragm, a plano-convex lens and a polarizing film are arranged on an incident light path of the sensor in sequence; a convex lens and a CCD (charge coupled device) video camera are arranged on an emergent light path of the sensor in sequence, and the CCD video camera and the precision monochrometer are connected with a computer. According to a system, the SPR spectrum resonance curves of the lattice points are obtained in parallel by an optical sweeping mode, different biological targets are detected in parallel according to the motion of the SPR peaks of the lattice points, and the sensor has single-channel SPR sensitivity while being used for multichannel parallel detection.

A portable penetrating surface plasmon resonance biosensor and its preparation and detection method, the sensor includes a strip of optical glass, the bottom of which is coated with a metal internal reflection film, the side wall is coated with a chromium film, and the nano-chromium film is coated with biological The gold film of the molecular sensitive layer, and the incident optical path of the strip optical glass is equipped with a collimator, and the outgoing optical path is sequentially equipped with a polarizer, an optical fiber collimator, and a fiber optic spectrometer connected to the optical fiber collimator through a multimode optical fiber. Metal internal reflection film is coated on the bottom of the strip optical glass; chrome film and gold film are sequentially plated on the side wall of the top; a biomolecular sensitive layer is formed on the surface of the gold film; and sealed with BSA solution to obtain a penetrating surface plasma Body resonance biosensors. The invention inserts the test tube into the liquid for SPR measurement, simplifies the system and realizes the portability of the sensor. At the same time, it overcomes the defects of the existing probe-type SPR sensor, and its measurement method is much simpler, realizing the portability of the instrument.

A surface plasmon resonance high-refractive index sensor based on a porous aluminum film structure comprises a quartz waveguide, a porous metal aluminum film layer arranged on the upper surface of the quartz waveguide, an incident light source and a detector. The invention adopts a porous metal layer structure as a sensing structure, which significantly enhances the surface plasmon resonance intensity and greatly improves the sensitivity of the sensor. The porous structure of the present invention forms a micro-nano sensing structure, which can be simultaneously applied to surface plasmon resonance biosensors for monitoring high refractive index and low refractive index.