Portable probing type surface plasma resonance biosensor and preparation method and detection method thereof

Abstract

The invention relates to a portable probing type surface plasma resonance biosensor and a preparation method and a detection method of the portable probing type surface plasma resonance biosensor. The sensor comprises strip optical glass, an inner meal reflecting film coats the bottom of the strip optical glass, a chromium film coats the side wall of the strip optical glass, a gold film coated with a biomolecular sensitive layer coats the nano chromium film, a collimator is arranged on an incident light path of the strip optical glass, and a polarizing film, an optical fiber collimator and an optical fiber spectrometer connected with the optical fiber collimator by a multimode optical fiber are sequentially arranged on an emergent light path. The inner meal reflecting film coats the bottom end of the strip optical glass, the chromium film and the gold film sequentially coat the side wall of the top part of the strip optical glass, the biomolecular sensitive layer is formed on the surface of the gold film, and BSA solution is used for sealing to obtain the probing type surface plasma resonance biosensor. The portable probing type surface plasma resonance biosensor is inserted into liquid in a test tube for SPR measurement, the system is simplified, and the portability of the sensor is achieved; and besides, the portable probing type surface plasma resonance biosensor overcomes the disadvantages of the existing probing type SPR sensor, the measurement mode is simple and the portability of the instrument is realized.

Description

technical field [0001] The invention relates to a surface plasmon resonance biosensor, in particular to a portable penetrating surface plasmon resonance biosensor and a preparation and detection method thereof. Background technique [0002] Surface plasmon resonance (SPR) is a physical optical phenomenon produced by the coupling of light beams through the surface of a medium and metal thin films. In 1902, Wood first discovered this phenomenon in optical experiments. One of the necessary conditions for the formation of SPR is the existence of the interface between the metal and the dielectric. When the parallel light on the parallel surface is incident at an angle greater than the critical angle of total reflection, if its frequency is consistent with the frequency of free electrons (ie, plasma) oscillating on the metal surface, The incident light will be coupled into the surface plasmon. At this angle, the surface plasmon resonance will cause significant absorption of the e...

AI Technical Summary

Problems solved by technology

The existing problems of fiber optic SPR sensors are: the sensitivity and signal-to-noise ratio of multimode fiber SPR are not high, and the resonance wavelength is dispersive; while the core diameter of single-mode fiber is small, the coupling light is weak

In addition, the preparation process of fiber optic SPR sensing probes is more complicated than that of planar sensor sheets.

In order to overcome the above problems, sensor probes processed with optical glass rods instead of optical fibers appeared (Takuo Akimoto, Analytica Chimica Acta 610(2008) 119–124), but beam splitters are required, and the reflected light is less than half One of the light intensity enters the spectrometer, and the signal is lost by more than half

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