The invention provides an automatic high-flux SPR detector. The automatic high-flux SPR detector comprises a laser device, an SPR exciting light group, an SPR biosensor, a detection signal collector,a control and analysis unit, a flow pipeline, an automatic sampling device, an automatic cleaning device and an automatic waste collection device, wherein the laser device, the SPR exciting light group and the SPR biosensor are connected with the control and analysis unit through the detection signal collector, and the flow pipeline is connected with the SPR biosensor, the automatic sampling device and the automatic cleaning device. The device can detect a plurality of indexes of a plurality of samples simultaneously; biochips and sampling needles can be recycled, so that the cost is reduced;a reference channel is arranged, so that the influence of non-specific binding, sample composition, temperature and other factors on result analysis is reduced; cleaning, sampling and waste collectionare fully automatic; parts are convenient to replace, and the service life is long.