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Fiber SPR biosensor and preparation method thereof

A biosensor and sensor technology, applied in the field of biosensors, can solve the problems of high detection limit, low sensitivity of optical fiber SPR biosensor, and lower detection limit, and achieve the effects of lower detection limit, enhanced surface electric field, and enhanced stability

Pending Publication Date: 2020-02-07
NORTHEASTERN UNIV
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Problems solved by technology

[0004] In order to solve the problems of low sensitivity and high detection limit of existing optical fiber SPR biosensors, the present invention proposes an optical fiber SPR biosensor and a preparation method thereof, the sensor is based on a U-shaped optical fiber molybdenum disulfide-gold film sandwich sensing structure , so that the sensitivity has been significantly improved and the detection limit has been significantly reduced

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  • Fiber SPR biosensor and preparation method thereof
  • Fiber SPR biosensor and preparation method thereof
  • Fiber SPR biosensor and preparation method thereof

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Embodiment 1

[0046] In this example, a U-type fiber-based molybdenum disulfide-gold film sandwich fiber SPR biosensor for detecting human immunoglobulin IgG is prepared, that is, the antigen to be detected is human immunoglobulin IgG, and the antibody to be detected is goat anti- Human immunoglobulin.

[0047] Such as figure 1 As shown, an optical fiber SPR biosensor adopts a U-shaped fiber-based molybdenum disulfide-gold film sandwich sensing structure, including a U-shaped multimode fiber of 30 to 40 cm, and the U-shaped multimode fiber is bent in the middle Part of it is the sensing area. The length of the sensing area is 20-30mm. The surface of the optical fiber in the sensing area is fixed with the molybdenum disulfide coating 3 through electrostatic self-assembly. The thickness of the molybdenum disulfide coating is 0.65-13nm. The surface of the layer is plated with gold film 4, the thickness of the gold film layer is 40-55nm, the surface of the gold film is cured with an antibody-goat ...

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Abstract

The present invention provides a fiber SPR biosensor and a preparation method thereof. A sensing structure of molybdenum disulfide-gold film interlayer based on U-type fiber is used for the sensor. Acentral curved portion of the U-type fiber is a sensing area. A molybdenum disulfide coating is coated on a sensing area fiber surface. A gold film layer is coated on a surface of the molybdenum disulfide coating. Antibodies are fixed on a gold film surface by using dopamine. According to the SPR biosensor provided in the present invention, a fiber-molybdenum disulfide-gold film interlayer structure is used. Molybdenum disulfide has a greater band gap and higher light absorption efficiency, can stimulate a stronger SPR effect and enhance a surface field of the sensor, and can effectively improve sensitivity of the sensor. Antibodies are fixed on a sensor surface by using dopamine. Measurement with high antigen sensitivity and a low detection limit is implemented, so that the sensor is applied more widely in the biological detection aspect.

Description

Technical field [0001] The invention relates to the technical field of biosensors, in particular to an optical fiber SPR biosensor and a preparation method thereof. Background technique [0002] In recent years, the application of surface plasmon resonance (SPR) technology in various fields has set off a research boom, such as food safety testing, environmental monitoring, medical diagnosis, and bioengineering. In particular, the application of SPR in biosensing technology has attracted extensive attention and research. Various high-performance biosensors have appeared one after another. Among them, optical fiber biosensors are popular due to their small size, label-free, and high detection speed. Favor. Surface plasma resonance (SPR) biosensor is a kind of sensor that develops rapidly and more comprehensively among optical sensors, but SPR biosensor uses metal with negative dielectric constant as the excitation element, which will cause the internal loss of the sensor Increase...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/552G01N21/75G01N33/551G01N33/553G01N33/68
CPCG01N21/553G01N21/75G01N33/551G01N33/553G01N33/6854
Inventor 王琦宋行张洪鑫孙卓任子洋
Owner NORTHEASTERN UNIV
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