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Automatic high-flux SPR detector

A detector and high-throughput technology, applied in the direction of chemical instruments and methods, instruments, measuring devices, etc., can solve the problems of high cost, low flux, and difficulty in distinguishing non-specific adsorption, and achieve long service life, easy replacement, cost reduction effect

Pending Publication Date: 2020-01-17
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing SPR technology still needs to make efforts to overcome the shortcomings of low throughput, high cost, difficulty in distinguishing non-specific adsorption, and strict requirements on temperature and sample composition.

Method used

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Embodiment 1

[0029] In order to describe the technical content, structural features, achieved goals and effects of the present invention in detail, the following will be described in detail in conjunction with the embodiments and accompanying drawings.

[0030] see figure 1 as well as Figure 9 , the present invention provides an automatic high-throughput SPR detector, comprising a laser 1, an optical group 2 for exciting SPR, an SPR biosensor 3, a detection signal collector 4, a control and analysis unit 5, a flow pipeline 6, and an automatic sampling Device 7 and automatic cleaning device 8, automatic waste collection device 9, laser 1, optical group 2 for exciting SPR, SPR biosensor 3 are connected to control and analysis unit 5 through detection signal collector 4, and flow pipe 6 is connected to SPR biosensor 3 , Automatic sampling device 7 and automatic cleaning device 8.

[0031]In this embodiment, the light group 2 for exciting SPR includes a beam expander 201, a collimator 202, ...

Embodiment 2

[0039] The biochip 302 is arranged in an array of at least 5 rows*3 columns, each row is a channel, and a sample is detected, and the immobilized biosensitive molecules in each column are antibody 1, no antibody, and antibody 2 from left to right. The column without antibody is used as a reference to eliminate the influence of the difference in refractive index of different samples, or other factors such as temperature on the result analysis. The biochip 302 includes a base layer and a coating layer arranged on the surface of the base layer. The biochip 302 also has a flow cell 302, at least five sample inlets, and at least five sample outlets. The sample inlets correspond to the sample outlets one by one. The sampling port is connected with the sampling needle 20 through the flow hose 24 . The base layer is a silicon chip, and the silicon chip is coated with a gold film or a composite film, and the carboxylated dextran layer is fixed by covalent coupling on the silicon chip c...

Embodiment 3

[0041] The sampling needle 20 is much larger than the flow pipe 6, and can produce a large enough opening after piercing, so as to facilitate the dumping of waste liquid in the later stage. An easy-to-replace rubber gasket 17 is arranged above the sampling needle 20, and the cleaning notch matches the rubber gasket to ensure the tightness between the sampling port and the sampler interface, and the gas siphon unit can work normally to complete the sample injection. The rubber gasket 17 is an annular ring whose lower diameter is slightly smaller than that of the upper part. When the three-dimensional motion mechanical arm 25 moves down in the Z direction and squeezes the rubber gasket 17, the elasticity of the rubber is used to make the interface well sealed; Matching, the structure is the same as the thermos cup lid. When the rubber sealing gasket 17 is aging, it is easy to replace, maintain good sealing performance, and prolong the service life of the whole system.

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Abstract

The invention provides an automatic high-flux SPR detector. The automatic high-flux SPR detector comprises a laser device, an SPR exciting light group, an SPR biosensor, a detection signal collector,a control and analysis unit, a flow pipeline, an automatic sampling device, an automatic cleaning device and an automatic waste collection device, wherein the laser device, the SPR exciting light group and the SPR biosensor are connected with the control and analysis unit through the detection signal collector, and the flow pipeline is connected with the SPR biosensor, the automatic sampling device and the automatic cleaning device. The device can detect a plurality of indexes of a plurality of samples simultaneously; biochips and sampling needles can be recycled, so that the cost is reduced;a reference channel is arranged, so that the influence of non-specific binding, sample composition, temperature and other factors on result analysis is reduced; cleaning, sampling and waste collectionare fully automatic; parts are convenient to replace, and the service life is long.

Description

technical field [0001] The invention belongs to the technical field of photoelectric sensing and detection, and in particular relates to an automatic high-flux SPR detector. Background technique [0002] Surface Plasma Resonance (SPR) is a physical optical phenomenon. When the light wave enters the metal film from the glass, total internal reflection will occur at the interface, but the component of the light wave - P polarized light (a kind of transverse magnetic wave) will penetrate into the metal film to a certain depth, resulting in the so-called evanescent wave, and then trigger Free electrons near the metal surface oscillate and form surface plasmons, exciting the SPR phenomenon. When the surface plasmon and the evanescent wave meet the resonance condition, that is, the frequency of the two is equal, the total reflection condition at the interface will no longer hold. Part of the energy of the incident light is absorbed by the electrons near the metal surface, so tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/552G01N21/75G01N33/543B08B3/02
CPCG01N21/553G01N21/75G01N33/543B08B3/02
Inventor 彭晓李可意袁玉峰李林潼许楠林周颖欣于德良
Owner SHENZHEN UNIV
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