Angle modulation mechanism for modularization of optical surface plasma resonance biosensor

Abstract

The invention relates to an angle modulation mechanism for modularization of an optical SPR (surface plasma resonance) biosensor. The angle modulation mechanism comprises a chip module, a laser regulating unit, a CCD (charge coupled device) regulating unit and a rack; wherein the laser regulating unit comprises a semi-circle card, an arm swinging frame, an idler wheel and a sliding rail, the arm swinging frame is movably installed on the rack by means of a rotation shaft, the semi-circle card is installed on the arm swinging frame, the idler wheel is installed at a lower side of the arm swinging frame and cooperates with the sliding rail which is arranged below the arm swinging frame to regulate a swinging angle of the arm swinging frame; a corresponding optical path detection channel is formed between laser, a chip component and a CCD image sensor. The angle modulation mechanism is delicate in structure, location is accurate, consistency regulation of an optical path is convenient; tightness of a pressure between a microflow pool and the chip module is convenient to regulate, so that the microflow pool is in close contact with an Au film, so as to not only prevent leakage of a sample solution but also prevent the Au film being damaged due to an excessive pressure; and furthermore, the microflow pool is convenient to ascend, and after the microflow pool is ascended and reset, accurate location between the microflow pool and a biological chip can be kept.

Description

technical field [0001] The invention relates to the field of optical surface plasmon resonance (Surface plasmon resonance, SPR) biosensors, in particular to an optical SPR biosensor modular angle modulation mechanism. Background technique [0002] In the past 10 years, optical SPR biosensing technology has achieved rapid development in terms of implementation methods, instrument development and application field expansion. The detection mechanism of the optical SPR biosensor is as follows: the recognition film of biomolecules is fixed on the surface of the Au film of the optical SPR sensor, and the molecular recognition film captures the measured analyte, causing the refractive index of the surface of the sensor Au film to change, so it can be detected without labeling molecules. Test directly to realize non-destructive real-time online detection. Optical SPR biosensors include optical prism type, optical waveguide type, grating type, etc. according to the body of the excit...

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Problems solved by technology

For example, the Chinese patent document CN101539570A discloses a coupling device for an optical SPR biochip, a sensor, and a microfluidic cell, but the device can only be coupled with an integrated biosensor imported from abroad, resulting in a high overall cost of the instrument, which will inevitably increase the use of users , maintenance cost, it is difficult to be widely used; CN102095684A discloses a multi-degree-of-freedom adjustment mechanism for optical surface plasmon resonance biosensors, but its laser light source is fixed, and the adjustable angle range of incident light is limited, so it is difficult to meet the needs of different material components. Detection needs; CN102410989A discloses a dual-channel multi-degree-of-freedom adjustment mechanism for optical surface plasmon resonance biosensors. Although it can perform multi-degree-of-freedom adjustments, its operating procedures are more complicated, and the reproducibility of the test phenomenon adjustment position is difficult to achieve , the efficiency of instrument calibration and detection is difficult to satisfy

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