The present invention relates to a qualitative and quantitative method for a
thio thiolation post-translational modification
peptide segment. The method is divided into two routes such as a
route A and a
route B, wherein the
route A comprises that
a peptide segment produced through
proteolysis and iodoacetamide beads are incubated, the mercapto
peptide segment and the
thio mercapto
peptide segmentare enriched, the
disulfide bond of the
thio mercapto peptide segment is reduced and released with a
reducing agent, the released thio mercapto peptide segment can be labeled with a cleavable isotopeaffinity tag, and finally the linking arm of the cleavable
isotope affinity tag is cleaved with a
lysis liquid, and the route B comprises that
protein containing mercapto and thio mercapto is labeledwith a cleavable
isotope affinity tag,
proteolysis is performed, the mercapto peptide segment and the thio mercapto peptide segment are subjected to affinity enrichment, and finally the linking arm of the cleavable
isotope affinity tag linked to the mercapto peptide segment and the thio mercapto peptide segment is cleaved with a
lysis liquid. According to the present invention, the qualitative and quantitative method has advantages of good selectivity, low false positive and the like, and can achieve the qualitation and the quantification of the scaled thio thiolation peptide segment in the complex
proteome sample.