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47 results about "Low molecular weight protein" patented technology

The ras protein belongs to a large super-family of proteins known as "low-molecular weight G-proteins". These proteins are called "G-proteins" because they bind guanine nucleotides (GTP and GDP).

EphA2, EphA4 and LMW-PTP and methods of treatment of hyperproliferative cell disorders

The present invention relates to methods and compositions designed for treatment, management, or prevention of a hyperproliferative cell disease, particular cancer. The methods of the invention comprise the administration of an effective amount of a composition that targets cells expressing low molecular weight protein tyrosine kinase (“LMW-PTP”) in particular using moieties that bind an Eph family receptor tyrosine kinase, such as EphA2 or EphA4, and inhibits or reduces LMW-PTP expression and / or activity. In one embodiment, the method of the invention comprises administering to a subject a composition comprising an EphA2 or EphA4 targeting moiety attached to a delivery vehicle, and one or more agents that inhibit LMW-PTP expression and / or activity operatively associated with the delivery vehicle. In another embodiment, the method of the invention comprises administering to a subject a composition comprising a nucleic acid comprising a nucleotide sequence encoding an EphA2 or EphA4 targeting moiety and an agent that inhibits or reduces LMW-PTP expression and / or activity. In yet another embodiment, the method of the invention comprises administering to a subject a composition comprising an EphA2 or EphA4 targeting moiety and a nucleic acid comprising a nucleotide sequence encoding an agent that inhibits or reduces LMW-PTP expression and / or activity, where the nucleic acid is operatively associated with the delivery vehicle. Pharmaceutical compositions are also provided by the present invention.
Owner:MEDIMMUNE LLC

System and methods for electrophoretic separation of proteins on protein binding membranes

Proteins can be rapidly separated to a high degree of resolution by electrophoresis on polymeric membranes that have high protein binding capacity. The electrophoretic separation is carried out in a low conductivity, water-miscible organic solvent buffer. The low conductivity of the organic solvent buffer minimizes heat generation, and the water-miscible nature of the organic solvent buffer permits the analysis of hydrophobic and low molecular weight proteins as well as hydrophilic proteins. When electrophoresis is conducted under non-denaturing conditions, it allows the detection of enzymatic activities, protein-protein interactions and protein-ligand interactions.
Owner:TEMPLE UNIVERSITY

Isolated Soy Protein Having High Molecular Weight Protein Fractions and Low Molecular Weight Protein Fractions

Isolated soy proteins for producing high protein food bars having improved functionality are disclosed. The isolated soy proteins comprise both high molecular weight protein fractions and low molecular weight protein fractions. The isolated soy proteins produce high protein food bars having an improved texture and extended shelf-life as compared to conventional high protein food bars.
Owner:SOLAE LLC

Proteins from actinobacillus pleuropneumoniae

The present invention is directed to five novel, low molecular weight proteins from Actinobacillus pleuropneumoniae (APP), which are capable of inducing, or contributing to the induction of, a protective immune response in swine against APP. The present invention is further directed to polynucleotide molecules having nucleotide sequences that encode the proteins, as well as vaccines comprising the proteins or polynucleotide molecules, and methods of making and using the same.
Owner:PFIZER INC +1

Indigenous microbial sustained-release long-acting nutrient for oil recovery

The invention belongs to the field of the petroleum industry, and particularly relates to a sustained-release long-acting nutrient for improving the valid period of indigenous microbial oil recovery. The invention adopts the technical principle that the conventional nutrient is prepared from water-soluble raw materials such as diammonium hydrogen phosphate, urea, ammonium nitrate, sodium nitrate, amino acid, molasses and the like. The raw materials are water-soluble, have different adsorption capacities in a stratum and different moving speeds, are easily drained and lost, and have short valid period. The nutrient is immobilized by adopting an immobilization method with hydrogel and the like and slowly released in the stratum; and organic phosphate, low molecular weight protein powder and the like are slowly hydrolyzed in the stratum to generate the nutrient required by indigenous bacteria. The using concentration of the sustained-release nutrient is 0.2 to 2.0 percent. The valid period of indigenous bacteria oil recovery is prolonged, so that the nutrient can enter the deep part of an oil deposit, and the efficiency of the indigenous microbial oil recovery is improved.
Owner:YANGTZE UNIVERSITY +1

EphA2, EphA4 and LMW-PTP and methods of treatment of hyperproliferative cell disorders

The present invention relates to methods and compositions designed for treatment, management, or prevention of a hyperproliferative cell disease, particular cancer. The methods of the invention comprise the administration of an effective amount of a composition that targets cells expressing low molecular weight protein tyrosine kinase (“LMW-PTP”) in particular using moieties that bind an Eph family receptor tyrosine kinase, such as EphA2 or EphA4, and inhibits or reduces LMW-PTP expression and / or activity. In one embodiment, the method of the invention comprises administering to a subject a composition comprising an EphA2 or EphA4 targeting moiety attached to a delivery vehicle, and one or more agents that inhibit LMW-PTP expression and / or activity operatively associated with the delivery vehicle. In another embodiment, the method of the invention comprises administering to a subject a composition comprising a nucleic acid comprising a nucleotide sequence encoding an EphA2 or EphA4 targeting moiety and an agent that inhibits or reduces LMW-PTP expression and / or activity. In yet another embodiment, the method of the invention comprises administering to a subject a composition comprising an EphA2 or EphA4 targeting moiety and a nucleic acid comprising a nucleotide sequence encoding an agent that inhibits or reduces LMW-PTP expression and / or activity, where the nucleic acid is operatively associated with the delivery vehicle. Pharmaceutical compositions are also provided by the present invention.
Owner:MEDIMMUNE LLC

Kidney target precursor medicine, said prepn., its preparing method and application

A precursor medicine targetting to kidney is composed of the low-molecular protein chosen from lysozyme, insulin, cytochrome C and pepetide suppressing enzyme or its polypeptide fragment, and the extract of common threewingnut root or its derivative, or glucocorticoid. It can be used as the immune depressant for treating renal disease, or the medicines for treating the rejection reaction and nephritis.
Owner:SICHUAN UNIV

Magnetic mesoporous silica core-shell structure affinity microsphere based on metal ion modification as well as preparation method and application thereof

The invention relates to the technical field of a functional material, and discloses a preparation method of a magnetic mesoporous silica core-shell structure affinity microsphere based on metal ion modification. The method comprises the following steps of: 1) preparing magnetic particles from hexahydrate ferric trichloride; 2) preparing particle dispersion liquid by utilizing the magnetic particles; 3) adding ethanol solution containing ethyl silicate and titanium tetrabutoxide to the particle dispersion liquid, carrying out ultrasound, agitation and magnetic separation, and removing template molecules to obtain the magnetic mesoporous silica core-shell structure taking the magnetic particles as a core and the SiO2 as a shell. Polypeptide and protein with low molecular weight can be gathered and purified from a complicated biological sample by utilizing the magnetic mesoporous silica core-shell structure affinity microsphere based on the metal ion modification. The purification and separation processes of complicated low-abundance protein and low-molecular weight protein sample are greatly simplified by utilizing the magnetic property of an affinity material; and the operation time is also shortened.
Owner:SHENZHEN UNIV +1

Highly soluble, high molecular weight soy bean protein

A soy protein with a high molecular weight. The high molecular weight soy protein has desirable flavor and functional properties, such as high water solubility and emulsification and low sedimentation and viscosity. The method of manufacturing the protein uses soy flour and aggregates its low molecular weight proteins into high molecular weight proteins without using aqueous alcohol to modify the structure of the protein.
Owner:SOLAE LLC

Composition and method for hair straightening

A composition and method of use therefor that allows for permanent straightening or curl of human hair that is not irritating to the skin, that does not have an obnoxious odor, that is significantly less damaging to hair, that allows for the immediate shampooing of hair thereafter and that provides for re-treatment of the hair without additional damage, primarily using urea and high temperatures to break and reform disulfide bonds as a means to allow for the penetration of low molecular weight proteins into the shaft of the hair.
Owner:SAUTE ROBERT +1

Association between the acid phosphatase (ACP1) gene and Alzheimer's disease

Alzheimer's disease is characterized by the presence of senile plaques formed from beta amyloid (Abeta), and neurofibrillary tangles (NTFs) formed from paired helical filaments consisting of hyperphosphorylated tau. A number of studies have shown that the NTFs correlate better with the duration and severity of Alzheimer's disease than senile plaques. However, a criticism of the primary etiological role of NTFs in Alzheimer's disease is the absence of variants of kinases or phosphatases associated with Alzheimer's disease. Acid phosphatase, a product of the ACP 1 gene, is a ubiquitous low molecular weight protein tyrosine phosphatase. A common allele, ACP 1*A, is associated with a lower activity of acid phosphatase. It is due to an Arg 105 Gln substitution of the ACP1 locus and detected as a Taq I polymorphism. We report a significant association of the low activity 2 allele with sporadic early onset Alzheimer's disease (EOAD). These findings support the possibility that other variants of kinase and genes may be associated with sporadic Alzheimer's disease.
Owner:CITY OF HOPE

Kit for detecting low-abundance and low-molecular-weight protein spectrum

The invention discloses a kit for detecting low-abundance and low-molecular-weight protein spectrums. The kit comprises a kit box; a separator, a protein separation and enrichment material, a diluting buffer solution and a cleaning buffer solution are arranged in the kit body; and the protein separation and enrichment material is disposed in the separator and are porous silicon particles, the channel diameter of the material is 1-50nm, and the channel surface of the material has a chemically modified group which is an anion exchange group, a cation exchange group, a hydrophobic group or a polar group. The kit reaches an effect of one-step separation, enrichment and detection of low-molecular-weight proteins in a serum sample through preparing the porous silicon particles in the invention. The kit disclosed in he invention has the advantages of simplicity, portability, and accurate and fast detection method.
Owner:HANGZHOU WELL HEALTHCARE TECH CO LTD

Soy phytochemical composition

Disclosed is a soy phytochemical composition obtained by removing soluble components out of acidified soy molasses by settling, decantation and / or centrifugation to obtain a precipitated residue sediment containing not less than 6% of isoflavones, not less than 18% of saponins and not less than 13% of crude low molecular weight protein material on a dry matter basis.
Owner:CHAJUSS DANIEL

Preparation method of low-abundance low-molecular-weight protein-rich material

The invention discloses a preparation method of a low-abundance low-molecular-weight protein-rich material. The preparation method comprises the following steps of: fixing a P-type boron-doped silicon wafer in an electrolytic cell; adding ethanol and hydrofluoric acid as electrolyte; performing direct current electrolytic etching and demolding by taking the silicon wafer as an anode and a platinum electrode as a cathode; cleaning an etched and demolded porous silicon layer with ethanol, then ultrasonically crushing and blowing with nitrogen; performing ozone oxidation treatment on porous silicon particles; soaking the oxidized porous silicon particles in mixed solution of silylation reagent and organic solvent to react, then drying in a drying box, and soaking and cleaning the porous silicon particles, which are dried by evaporation, with ethanol; and selecting porous silicon with required aperture and performing surface modification by using amino silylation reagent, sulfydryl silylation reagent or epoxy silylation reagent. According to the preparation method, the effect of separating, enriching and detecting low-molecular-weight protein in a blood serum sample in one step is achieved by preparing the porous silicon particles.
Owner:ZHEJIANG UNIV

Metabolic enzyme activity and disulfide bond reduction during protein production

The present disclosure relates to the use of host cell protein biomarkers to assess disulfide bond reduction in compositions comprising a protein of interest. In some embodiments, the disclosure relates to methods of predicting the occurrence of disulfide bond reduction or low molecular weight protein species in compositions comprising a protein of interest, wherein the expression or activity level of at least one host cell protein is measured and provides a benchmark value associated with the occurrence of disulfide bond reduction or low molecular weight species of said protein of interest. In some embodiments, the disclosure relates to methods of producing a protein of interest, wherein host cells capable of producing the protein of interest are cultured, the expression or activity level of at least one host cell protein is measured, and downstream isolation of the protein of interest is informed by the host cell protein measurements.
Owner:BRISTOL MYERS SQUIBB CO

Novel proteins from Actinobacillus pleuropneumoniae

The present invention is directed to five novel, low molecular weight proteins from Actinobacillus pleuropneumoniae (APP), which are capable of inducing, or contributing to the induction of, a protective immune response in swine against APP. The present invention is further directed to polynucleotide molecules having nucleotide sequences that encode the proteins, as well as vaccines comprising the proteins or polynucleotide molecules, and methods of making and using the same.
Owner:ANKENBAUER ROBERT G +6

Antler herb medicine fermented with chicken gizzard and a method for preparation thereof

Disclosed are an antler herb medicine and a preparing method thereof. The antler herb medicine is prepared by fermenting a mixture of antler and gizzard membrane at 20-60° C. In the mixture, the weight ratio of antler to gizzard membrane is within the range of 1:10 to 10:1. The antler herb medicine contains all of the efficacious components of antler, rather than selected components. Also, the fermentation of antler brings about a great change in the composition of antler, converting high molecular weight proteins into low molecular weight proteins and polypeptides, so that the antler herb medicine is greatly improved in uptake rate in the body. Thus, the antler herb medicine can show medicinal effects of antler at a small amount.
Owner:LEE YOUN SOO

PM2.5 air filter element and preparation method thereof

The invention discloses a PM2.5 air filter element and a preparation method thereof, and relates to the field of air purification. The PM2.5 air filter element comprises a filter element body and a PM2.5 capturing agent sprayed and coated on the filter element body, wherein the PM2.5 capturing agent uses water-soluble neutral or weak alkaline amino acid, polypeptide or low-molecular-weight protein as main raw materials; hydrophilic polymers and salt are used as the assistance; surfactants and polyamino compound are compounded for preparation. The dust can be effectively filtered; the capturing effect on organic harmful substances and sol type PM2.5 is obvious; the air purification effect is good; the cost is low; the filter element replacement or cleaning operation is simple. The PM2.5 air filter element can be widely applied to equipment such as an automobile air filter element or air purifier; the practical values are high.
Owner:深圳市亮一方光电有限公司

Diluent for fluorescent NANO particles, kit for immunofluorescent staining which utilizes same, solution for immunofluorescent staining, immunofluorescent staining method, and gene staining method

[Problem ] Provided is a means for detecting and quantifying a biological substance of interest with an improved accuracy by inhibiting non-specific adsorption of fluorescent nanoparticles and thereby reducing the background noise in immunostaining with fluorescent nanoparticles. [Means for Solution] Immunostaining is carried out upon diluting fluorescent nanoparticles with a fluorescent nanoparticle diluent which contains 1 to 5% (W / W) of a protein having a molecular weight of 40,000 or higher (e,g., BSA) and 1 to 3% (W / W) of a protein having a molecular weight of less than 40,000 (eg ., casein) and, when casein is used as a low-molecular-weight protein, it is preferred that the κ-casein content in the casein is 10% (W / W) or less and the ratio of α-casein and β-casein (α-casein:β-casein) contained in the casein is 40:60 to 60:40 ( taking the total amount of α-casein and β-casein as 100).
Owner:KONICA MINOLTA INC

Low molecular weight protein tyrosine phosphatase (LMW-PTP) as a diagnostic and therapeutic target

Low molecular weight protein tyrosine phosphatase (LMW-PTP) is identified as a novel diagnostic and therapeutic target in cancer diagnosis, prognosis and treatment. The invention provides diagnostic and treatment methods useful in connection with cancers expressing LMW-PTP and, optionally, EphA2 receptor. Also provided is a screening method that utilizes changes in the amount and / or activity of LMW-PTP to identify candidate cancer therapeutic agents that effectively target the oncoprotein EphA2.
Owner:PURDUE RES FOUND INC

Blood purifying apparatus

A blood purifying apparatus having excellent purification performance (elimination of low molecular proteins, etc.) and being contaminated with little endotoxins flowing from the dialyzate side. Attention was paid to the solute permeability coefficient alpha and water permeability performance Lp of blood purifying apparatus and the relation between alpha and Lp has been examined. As a result, a blood purifying apparatus having excellent purification performance and substantially being free from invasion of endotoxins was obtained by regulating the value alpha / Lp to 6x10<-7> or above, or regulating alpha within a range of 8x10<-5> to 1.5x10<-3>, and the value alpha x Lp to 2.4x10<-2> or less. Also, a blood purifying apparatus having excellent purification performance and substantially being free from invasion of endotoxins was obtained by regulating the invasion ratio which is obtained by the polymer invasion test to 10% or less. The blood purifying apparatus has an excellent blood purifying performance expressed typically by the elimination ratio of low-molecular weight proteins and is substantially free from invasion of endotoxins from the dialyzate side, which makes it useful in, for example, treating patients with renal dysfunction.
Owner:ASAHI KASEI MEDICAL CO LTD

Inhibitors of Low Molecular Weight Protein Tyrosine Phosphatase and Uses Thereof

Herein are provided, inter alia, compounds capable of modulating the level of activity of low molecular weight protein tyrosine phosphatase (LMPTP) and methods of using the same. In embodiments, the compound has a structure according to Formula (I-A).
Owner:SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INST +1

Method for extracting protein from vitamin K2 fermentation liquid

The invention discloses low molecular weight protein which is produced from vitamin K2 fermentation waste liquid, so that resource waste is reduced, and meanwhile, the problem of environmental pollution caused by the fermentation liquid is well solved; and a good application prospect is guaranteed. Meanwhile, a method provided by the invention is low in raw material cost, simple in equipment, convenient to operate, easy for implementation of large-scale production and capable of improving added value of the industry.
Owner:QINGDAO UNIV

Inhibitors of low molecular weight protein tyrosine phosphatase (LMPTP) and uses thereof

PendingUS20200338084A1Modulating level of activityOrganic chemistryPeptide/protein ingredientsInsulin signallingTyrosine
Protein tyrosine phosphatases (PTPs) are key regulators of metabolism and insulin signaling. As a negative regulator of insulin signaling, the low molecular weight protein tyrosine phosphatase (LMPTP) is a target for insulin resistance and related conditions. Described herein are compounds capable of modulating the level of activity of LMPTP, compositions, and methods of using these compounds and compositions.
Owner:SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INST

A magnetic mesoporous silica core-shell structure affinity microsphere based on metal ion modification and its preparation method and application

The invention relates to the technical field of a functional material, and discloses a preparation method of a magnetic mesoporous silica core-shell structure affinity microsphere based on metal ion modification. The method comprises the following steps of: 1) preparing magnetic particles from hexahydrate ferric trichloride; 2) preparing particle dispersion liquid by utilizing the magnetic particles; 3) adding ethanol solution containing ethyl silicate and titanium tetrabutoxide to the particle dispersion liquid, carrying out ultrasound, agitation and magnetic separation, and removing template molecules to obtain the magnetic mesoporous silica core-shell structure taking the magnetic particles as a core and the SiO2 as a shell. Polypeptide and protein with low molecular weight can be gathered and purified from a complicated biological sample by utilizing the magnetic mesoporous silica core-shell structure affinity microsphere based on the metal ion modification. The purification and separation processes of complicated low-abundance protein and low-molecular weight protein sample are greatly simplified by utilizing the magnetic property of an affinity material; and the operation time is also shortened.
Owner:SHENZHEN UNIV +1

Association between the acid phosphatase (ACP1) gene and Alzheimer's disease

Alzheimer's disease is characterized by the presence of senile plaques formed from beta amyloid (Abeta), and neurofibrillary tangles (NTFs) formed from paired helical filaments consisting of hyperphosphorylated tau. A number of studies have shown that the NTFs correlate better with the duration and severity of Alzheimer's disease than senile plaques. However, a criticism of the primary etiological role of NTFs in Alzheimer's disease is the absence of variants of kinases or phosphatases associated with Alzheimer's disease. Acid phosphatase, a product of the ACP1 gene, is a ubiquitous low molecular weight protein tyrosine phosphatase. A common allele, ACP1*A, is associated with a lower activity of acid phosphatase. It is due to an Arg 105 Gln substitution of the ACP1 locus and detected as a Taq I polymorphism. We report a significant association of the low activity 2 allele with sporadic early onset Alzheimer's disease (EOAD). These findings support the possibility that other variants of kinase and genes may be associated with sporadic Alzheimer's disease.
Owner:CITY OF HOPE

Nanoporous substrates for analytical methods

Nanoporous materials can be used to enrich samples for subsequent analysis of substances contained in the sample. The method is shown to enrich the yield of species in the low molecular weight proteome, allowing detection of small peptides in the low nanomolar range.
Owner:THE OHIO STATE UNIV RES FOUND +1

Inhibitors of low molecular weight protein tyrosine phosphatase (LMPTP) and uses thereof

ActiveUS20200055875A1Modulating level of activityOrganic active ingredientsOrganic chemistryInsulin signallingTyrosine
Protein tyrosine phosphatases (PTPs) are key regulators of metabolism and insulin signaling. As a negative regulator of insulin signaling, the low molecular weight protein tyrosine phosphatase (LMPTP) is a target for insulin resistance and related conditions. Described herein are compounds capable of modulating the level of activity of low molecular weight protein tyrosine phosphatase (LMPTP) and compositions, and methods of using these compounds and compositions.
Owner:SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INST

Method for extracting protein from [beta]-carotene fermentation liquid

The invention discloses low molecular weight protein which is produced from [beta]-carotene fermentation waste liquid, so that resource waste is reduced, and meanwhile, the problem of environmental pollution caused by the fermentation liquid is well solved; and a good application prospect is guaranteed. Meanwhile, a method provided by the invention is low in raw material cost, simple in equipment,convenient to operate, easy for implementation of large-scale production and capable of improving added value of the industry.
Owner:QINGDAO UNIV
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