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Novel proteins from Actinobacillus pleuropneumoniae

a technology of actinobacteria and proteins, applied in the field of animal health, can solve the problems of limited cross-protective response induced by natural infection, inability to identify possible differences between the specificity of antibodies during primary versus secondary responses, and inability to isolate individual components or combinations of individual components from apps

Inactive Publication Date: 2004-10-07
ANKENBAUER ROBERT G +6
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

"The present invention provides five novel proteins, designated Omp20, OmpW, Omp27, OmpA1, and OmpA2, that are useful as antigenic components in a vaccine to protect swine against APP. These proteins can also be used as diagnostic reagents to identify swine that have been infected with APP or have been vaccinated with the present invention. The invention also provides polynucleotide molecules that encode these proteins and peptide fragments of them. The invention further provides fusion proteins comprising these proteins and carrier or fusion partners. The invention also provides isolated polynucleotide molecules that contain the nucleotide sequence of the APP protein. Overall, the invention provides useful tools for the development of a vaccine and diagnostic reagents to protect swine against APP."

Problems solved by technology

However, the patterns of cross-reactivity / cross-protection induced by such components do not cover all twelve APP serotypes.
In addition, immunization with isolated individual components or combinations of individual components from APP have so far failed to confer protection from challenge with some heterologous serotypes (unpublished observations).
Thus, it can be postulated that the cross-protective responses induced by natural infection are limited to specific serotype clusters.
Such an approach does not allow the identification of possible differences between the antibody specificities represented during primary versus secondary responses, nor the identification of dominant specificities at the infection site that are likely to be responsible for protection upon secondary encounter with the pathogen.
Naive cells migrate relatively homogeneously to secondary lymphoid tissues, but they home poorly to non-lymphoid tissues.

Method used

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  • Novel proteins from Actinobacillus pleuropneumoniae
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  • Novel proteins from Actinobacillus pleuropneumoniae

Examples

Experimental program
Comparison scheme
Effect test

example 10

Animal Study to Test Efficacy of Various Antigen Combinations

10.1 Materials and Methods

[0218] Fifty apparently healthy, crossbred pigs (approximately 6.5 weeks of age) were obtained from a herd with no history of APP disease or vaccination. Animals were randomly assigned by litter and by ApxII cytolytic neutralization antibody titer to five groups of 10 pigs (98% of the animals had serum neutralization titers <1:200 prior to the initiation of study). Pigs were acclimated for one week prior to initiation of study.

[0219] Animals were vaccinated with 2 ml of the appropriate vaccine (APP proteins with pp and without signal sequence) or placebo by the intramuscular route (IM; left neck muscle) on day 0, when pigs were approximately 7.5 weeks of age. After 3 weeks, animals were boosted with a second 2 ml dose (IM; right neck muscle). TABLE 6 identifies the vaccines used for first and second vaccinations of the 5 groups of pigs.

6TABLE 6 Vaccine Group Vaccine Components A 75 .mu.g pp-OmpW 7...

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Abstract

The present invention is directed to five novel, low molecular weight proteins from Actinobacillus pleuropneumoniae (APP), which are capable of inducing, or contributing to the induction of, a protective immune response in swine against APP. The present invention is further directed to polynucleotide molecules having nucleotide sequences that encode the proteins, as well as vaccines comprising the proteins or polynucleotide molecules, and methods of making and using the same.

Description

[0001] This application claims priority from provisional application Serial No. 60 / 105,285, which was filed on Oct. 22, 1998, and which is incorporated by reference in its entirety herein.1. FIELD OF THE INVENTION[0002] The present invention is in the field of animal health, and is directed to vaccines that protect swine against Actinobacillus pleuropneumoniae. More particularly, the present invention is directed to novel antigenic proteins shared by multiple serotypes of A. pleuropneumoniae, DNA molecules encoding the proteins, vaccines against APP comprising the proteins, and diagnostic reagents.2. BACKGROUND OF THE INVENTION[0003] A. pleuropneumoniae (hereinafter referred to as "APP") is a Gram negative coccobacillus recognized as one of the most important swine pneumonic pathogens (Shope, R. E., 1964, J. Exp. Med. 119:357-368; Sebunya, T. N. K. and Saunders, J. R., 1983, J. Am. Vet. Med. Assoc. 182:1331-1337). Twelve different serotypes have been recognized which vary in geograp...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K39/00A61K39/102C07K14/285G01N33/569
CPCA61K39/00A61K39/102A61K2039/52C07K14/285G01N33/56911
Inventor ANKENBAUER, ROBERT G.BAARSCH, MARY JOCAMPOS, MANUELKEICH, ROBINROSEY, EVERETTSUITER, BRIANWARREN-STEWART, LYNN
Owner ANKENBAUER ROBERT G
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