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Monoclonal antibody of hemophilus parasuis resistant OMP5 (outer membrane protein), hybridoma cell strain HPS1E2 and application

A hybridoma cell line, HPS1E2 technology, applied in the field of immunology, to achieve the effect of strong versatility and high titer

Inactive Publication Date: 2013-01-16
广东省农业科学院兽医研究所
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Problems solved by technology

At present, there is no commercially available diagnostic method for antibody-based HPS detection in China

Method used

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  • Monoclonal antibody of hemophilus parasuis resistant OMP5 (outer membrane protein), hybridoma cell strain HPS1E2 and application
  • Monoclonal antibody of hemophilus parasuis resistant OMP5 (outer membrane protein), hybridoma cell strain HPS1E2 and application
  • Monoclonal antibody of hemophilus parasuis resistant OMP5 (outer membrane protein), hybridoma cell strain HPS1E2 and application

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Embodiment Construction

[0025] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Without departing from the spirit and essence of the present invention, any modifications or substitutions made to the methods, steps or conditions of the present invention fall within the scope of the present invention.

[0026] Unless otherwise specified, the chemical reagents used in the examples are all conventional commercially available reagents, and the technical means used in the examples are conventional means well known to those skilled in the art.

[0027] Main experimental materials and sources:

[0028] Actinobacillus pleuropneumoniae type 1 (APP259, purchased from China Veterinary Drug Administration); HPS type 3 strain (SW114, gifted by Dr. Pat Blackall, Queensland Animal Health Research Institute, Australia); HPS type 11 (H456, Queensland Animal Health, Australia Gift of Dr. Pat Blackall, Institute); Escherichia coli, ...

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Abstract

The invention discloses a monoclonal antibody of hemophilus parasuis resistant OMP5 (outer membrane protein), a hybridoma cell strain HPS1E2 and application. The hybridoma cell strain is preserved in a Chinese typical culture preservation center, and the preservation number is CCTCCC2012138. The monoclonal antibody prepared with the hybridoma cell strain is fine in specificity, high in valence and high in universality, is not in cross reaction with swine Escherichia coli, swine pasteurella, actinobcillus pleuropneumoniae, streptococcus suis and swine bordetella, the valence of the purified ELISA (enzyme-linked immuno sorbent assay) antibody can reach 1:409600, hemophilus parasuis with different serotypes can be detected, and the monoclonal antibody can be widely used for etiology diagnosis, serological test, immunological test, prevention and treatment and the like of hemophflussuis.

Description

technical field [0001] The invention belongs to the field of immunology, and in particular relates to a monoclonal antibody against the outer membrane protein OMP5 of Haemophilus parasuis, a hybridoma cell line and applications thereof. Background technique [0002] Haemophilus parasuis ( Haemophilus Parasuis , HPS) is a non-motile, small, NAD-dependent, polymorphic Gram-negative bacillus belonging to the Pasteurellaceae family and the genus Haemophilus. HPS is a common bacterium that usually colonizes the upper respiratory tract of pigs and can often be isolated in the nasal cavity, tonsils, and anterior trachea without causing any clinical symptoms. HPS is also the causative agent of Glasser's disease in pigs. Under certain conditions, HPS can cause a systemic infection characterized by fibrinous polyserositis, arthritis, and meningitis. Glaser's disease has become the main bacterial disease affecting the swine industry worldwide, causing huge losses to the swine indust...

Claims

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Application Information

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IPC IPC(8): C12N5/20C07K16/12G01N33/577G01N33/569
Inventor 李春玲
Owner 广东省农业科学院兽医研究所
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