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Method for extracting protein from vitamin K2 fermentation liquid

A vitamin and protein technology, applied in the field of protein extraction, can solve pollution and other problems, achieve the effect of solving pollution, reducing environmental pollution, and simple equipment

Inactive Publication Date: 2018-02-09
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The technology of the invention not only reduces the waste of resources, but also solves the problem of environmental pollution caused by fermentation liquid, and has a good application prospect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Carry out ultrafiltration treatment with vitamin K2 fermented liquid with ultrafiltration membrane, the macromolecular substances such as mycelium and protein wherein are intercepted from waste liquid, collect interception component; The molecular weight cut-off of described ultrafiltration membrane is 3000 Dalton, water inlet pressure 1.1Mpa, ultrafiltration temperature 20°C, fermentation broth pH 7.2;

[0026] (2) Add water to the retained components obtained by ultrafiltration in step (1) and stir evenly, then use ultra-low temperature quick-freezing technology to quickly freeze the retained component solution to obtain a suspension, and then use a high-pressure homogenizer to crush the obtained suspension The volume ratio of the added volume of water to the retained components is 1:2; the ultra-low temperature quick freezing temperature is -40°C, and the quick freezing time is 15 minutes; the pressure of the suspension passing through the high pressure homogenize...

Embodiment 2

[0033] (1) carry out ultrafiltration process with vitamin K2 fermented liquid with ultrafiltration membrane, intercept macromolecular substances such as mycelia and protein therein from waste liquid, collect interception component; The molecular weight cut-off of described ultrafiltration membrane is 3000 Dalton, water inlet pressure 1.2Mpa, ultrafiltration temperature 20°C, fermentation broth pH 6.4;

[0034] (2) Add water to the retained components obtained by ultrafiltration in step (1) and stir evenly, then use ultra-low temperature quick-freezing technology to quickly freeze the retained component solution to obtain a suspension, and then use a high-pressure homogenizer to crush the obtained suspension The volume ratio of the added volume of water to the retained components is 1:6; the ultra-low temperature quick freezing temperature is -60°C, and the quick freezing time is 15 minutes; the pressure of the suspension passing through the high pressure homogenizer is 250Mpa, ...

Embodiment 3

[0041] The quality detection of the recovered protein in embodiment 1 or 2, detects the mass distribution of protein in the recovered product, the results are shown in Table 1:

[0042] Table 1 Molecular weight distribution of recovered protein

[0043] Molecular Weight (Daltons)

[0044] As can be seen from the results of the above-mentioned quality inspection, in the protein obtained by the extraction method of the present invention, the protein content of molecular weight in the range of 1000-2000 Daltons is greater than 75%. After the protein is used as feed, It is easily absorbed by animals and has good effects.

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Abstract

The invention discloses low molecular weight protein which is produced from vitamin K2 fermentation waste liquid, so that resource waste is reduced, and meanwhile, the problem of environmental pollution caused by the fermentation liquid is well solved; and a good application prospect is guaranteed. Meanwhile, a method provided by the invention is low in raw material cost, simple in equipment, convenient to operate, easy for implementation of large-scale production and capable of improving added value of the industry.

Description

technical field [0001] The invention relates to a method for extracting protein from vitamin K2 fermentation liquid, in particular to the production technology of collecting and crushing mycelium and separating and extracting protein. Background technique [0002] Vitamin K2 is a fat-soluble vitamin, a derivative of a naphthoquinone group with biological activity of phylloquinone, and is one of the indispensable and important vitamins in the human body. Vitamin K is a class of derivatives of naphthoquinone groups with phylloquinone biological activity. Vitamin K1 and K2 exist naturally. Vitamin K1 is widely found in green plants and is an important source of vitamin K in food. Vitamin K2 is mainly synthesized by intestinal bacteria. Synthetic vitamins are K3, K4, K5, K7 and so on. Vitamin K2 is biologically active in animals, and both vitamin K1 and vitamin K3 must be converted into vitamin K2 to function. All three forms of vitamin K are converted to vitamin K2 in the li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC07K1/34C12P21/06
Inventor 王艳玮施放王萍萍孙明周东超杨凯孙大鹏
Owner QINGDAO UNIV
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