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Preparation method of plant protein derivative with physiological activity and nutrition supplementing function

A technology of plant protein and physiological activity, which is applied in the preparation method of peptides, chemical instruments and methods, organic chemistry, etc., and can solve the problems of little research on peptides

Active Publication Date: 2013-08-28
SHANGHAI NONGLE BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are many studies on enzymatic hydrolyzates of plant proteins, but there are few studies on obtaining peptides rich in cysteine ​​residues through a series of treatments on plant proteins. It is hoped that the present invention can fill the gap in China and obtain a Preparation method of plant protein derivative with physiological activity and nutritional supplement function

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 The heat treatment of protein

[0036] Heat treatment can denature the protein and stretch the spherical structure, which is conducive to hydrolysis, but heat treatment may cause protein aggregation, and may also cause sulfhydryl-disulfide bond exchange to produce other forms of substances. Use differential scanning calorimetry (DSC) / non-reducing SDS-PAGE to analyze the degree of denaturation. On the premise of reaching the expected degree of denaturation, find the appropriate heating temperature and time to reduce the loss of disulfide bonds (determination of disulfide bonds content). DSC results show that the degree of denaturation of the protein solution can reach more than 90% when heated at 80°C for 20min; at 90°C for 5min; In addition, under these heat treatment conditions, there is almost no loss of protein disulfide bond content, so choose the best combination of temperature and time at 80°C for 20 minutes as heat treatment before protein enzymatic hy...

Embodiment 2

[0040] Embodiment 2 enzymatic hydrolysis method

[0041] 5% Glycinin solution after treatment, the temperature is controlled by circulating water bath at 60°C, the pH is controlled by a precision pH meter at 8.0±0.1 (during the hydrolysis process, 0.5M standard alkali is added dropwise to keep the pH constant), and 3% alkaline protease is hydrolyzed for 3 hours ; After alkaline protease hydrolysis, continue pepsin hydrolysis, adjust pH 2.0, control pH 2.0 ± 0.1 with a precision pH meter (during hydrolysis, add 0.5M hydrochloric acid to keep the pH constant), and control the temperature in a circulating water bath at 37 ℃, 2% pepsin hydrolysis for 2 hours, 5000g refrigerated centrifugation for 20 minutes, and the supernatant was obtained to obtain the desired crude peptide product. The degree of hydrolysis at the end of the hydrolysis process monitored by the pH-Stat method was about 20%.

Embodiment 3

[0042] The BH reduction method of embodiment 3 enzymolyzate

[0043] For the crude peptide product obtained in Example 2, add sodium borohydride (30% BH dissolved in 1M sodium hydroxide) to a final concentration of 1M, add 2 drops of octanol to defoam, react at 50°C for 30min, add concentrated hydrochloric acid ( to about pH2) to terminate the reaction and remove excess sodium borohydride and keep the system in a lower pH state to prevent oxidation of mercapto groups. The method of Example 5—4,4′-dithiodipyridine (4-DPS) method was used to quantitatively measure the sulfhydryl group and detect the reduction effect. Free thiols accounted for more than 95% of the total thiols.

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Abstract

The invention discloses a preparation method of a plant protein derivative with physiological activity and a nutrition supplementing function and belongs to the field of plant protein deep processing. According to the preparation method disclosed by the invention, the plant protein is used as a raw material and subjected to the processes of enzyme hydrolysis, clostridium thermoaceticum, biochemical separation and the like, and the product is a plant protein enzymatic hydrolysate with cysteine residue peptide as the important ingredient. According to the preparation method disclosed by the invention, abundant plant protein resource containing cysteine is used and subjected to enzymatic hydrolysis to obtain the plant protein derivative with the physiological activity and the nutrition supplementing function, and therefore the preparation method is of great significance.

Description

technical field [0001] The invention relates to a preparation method of a plant protein derivative with physiological activity and nutritional supplement function, and belongs to the field of deep processing of plant protein. Background technique [0002] Cysteine ​​is an aliphatic thiol-containing polar α-amino acid that can be converted from methionine in the body. Cysteine ​​has many physiological functions: it has a chelating effect in organisms, so it has a wide range of poisons such as formaldehyde, acetaldehyde, chloroform, carbon tetrachloride, lead, cadmium, methylmercury chloride, lipid peroxide, PCB , fugu poison, alcohol, etc. have effective detoxification effects, which have been proved by experiments; maintain the activity of important sulfhydryl enzymes in the keratin production of skin proteins, and supplement sulfhydryl groups to maintain normal metabolism of the skin and regulate the epidermis. The underlying melanin produced by the pigment cells in the lo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/16C07K1/18C07K1/22
Inventor 华欲飞孔祥珍王金艳丁秀臻张彩猛
Owner SHANGHAI NONGLE BIOLOGICAL PROD
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