56 results about "Bacillus acidiceler" patented technology

The invention relates to the cloning of a novel glutamate decarboxylase gene and the application thereof. A glutamate decarboxylase is obtained from bacillus, and then the glutamate decarboxylase is induced to express in escherichia coli. The study on the properties of the glutamate decarboxylase shows that, the glutamate decarboxylase is higher in activity at pH 4.0 to pH 6.0, and the optimum temperature of the glutamate decarboxylase is 45-65 DEG C. The Vmax of the glutamate decarboxylase is 150-200 U / mg, and the Km of the glutamate decarboxylase is 7-9 mmol / L. The recombinant escherichia coli containing the glutamate decarboxylase is subjected to whole-cell transformation, and then 500 g / L L-glutamic acid is added in batches. At 37 DEG C, the recombinant escherichia coli is converted for 12 hours in a sodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution at the pH 7.0, and 347.8 g / L gamma-aminobutyric acid is finally generated. The molar conversion rate is 99.4%. Compared with an existing glutamate decarboxylase, the above novel glutamate decarboxylase is higher in catalytic efficiency and relatively wide in pH range, thus being more suitable for industrial application.

The invention discloses a bacillus addisoni capable of producing neutral raw starch digesting amylase, belonging to the technical field of bioengineering. The wild bacillus strain capable of high-yield raw starch digesting amylase is selected from Guangxi cassava field shallow soil. The strain is preserved in China Center for Type Culture Collection in Wuhan university in Hubei province, with collection Number of being CCTCC M 2017320. The strain is simple and convenient in culturing conditions, short in fermentation period, high in enzyme activity; the produced raw starch digesting amylase isintermediate-temperature neutral enzyme, the most proper temperature and most proper pH distribution are respectively 50 DEG C and 7.0, the raw starch hydrolyzing capability is strong, and the product is mainly maltose. The bacillus addisoni has wide application prospects in the industrial fields of starch sugar, fermentation, food, feed and the like.

The invention relates to recombinant bacillus subtilis capable of synthesizing carotenoid 4,4'-diaponeurosporene with 30 carbon atoms (C30), and belongs to the biotechnology genetic engineering field. A bacillus subtilis expression plasmid pMK3-crtmn (which is capable of expressing C30 carotenoid synthases Crtm and Crtn in bacillus subtilis) containing C30 carotenoid synthase genes crtm and crtn is successfully constructed, and is successfully electro-transformed into bacillus subtilis WB800. The WB800 strain after transformation turns from the original color of white to yellow. A pigment component extracted from the recombinant WB800 is identified as 4,4'-diaponeurosporene by high performance liquid chromatography (HPLC). 4,4'-diaponeurosporene can stimulate maturation of dendritic cells, and generated cytokines (IL-6, IL-10, IL-12p70 and TNF[alpha]) have the amount increased, and moreover and have stronger ability to stimulate proliferation of T lymphocytes. With oral administration of the bacillus subtilis capable of synthesizing 4,4'-diaponeurosporene, the dextran sulphate sodium (DSS)-induced mice colitis symptoms can be significantly reduced. The bacillus subtilis capable of producing 4,4'-diaponeurosporene is expected to be developed as probiotics for prevention of colitis.

The invention discloses a nano emulsion for treating anaerobic infections of intestinal tracts of livestocks. The nano emulsion comprises the following raw materials in percent by mass: 0.1-8.0 percent of linaloe oil, 0.1-7.0 percent of carvacrol, 0.01-0.7 percent of metronidazole, 19.0-35.0 percent of surfactant, 0-5.0 percent of cosurfactant and the balance of distilled water, wherein the sum of the mass percent of the raw materials is 100 percent. The nano emulsion has stronger inhibiting and killing functions to most of obligate anaerobes, and is mainly used for treating enteritidis caused by infections of anaerobic bacteria such as bacteroides, clostridium, aerogenesis bacillus, peptococcus, eubacteria and streptococcus faecalis, for the livestocks. The nano emulsion has the advantages of high bioavailability, remarkable antibacterial action and treatment effect, and wide market prospect in the field of veterinary medicines.

A xyloglucanase having a relative xyloglucanase activity of atleast 50% at pH7 and either no or an insignificant cellulolytic activity is obtainable e.g. from a strain of Bacillus. A xyloglucanase comprising an amino acid sequence as shown in positions 30-261 of SEQ ID NO:2 or homologues may be derived from e.g. Bacillus licheniformis, ATCC 14580, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:1 from nucleotide 88 to nucleotide 783; and a xyloglucanase comprising an amino acid sequence as shown in positions 1-537 of SEQ ID NO:4 or homologues may be derived from e.g. B. agaradhaerens, NCIMB 40482, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:3 from nucleotide 1 to nucleotide 1611. The xyloglucanases are useful e.g. in cleaning compositions and for treatment of cellulosic fibres.

The present invention relates to the cloning and application of a novel glutamic acid decarboxylase gene. The glutamic acid decarboxylase obtained from Bacillus is induced and expressed in Escherichia coli and its properties are studied. It is found that the enzyme has a pH of 4.0 It has higher activity at ~pH6.0, the optimum temperature is between 45℃~60℃, Vmax is 150~200 U / mg, and Km is 7~9 mmol / L. The recombinant Escherichia coli containing the enzyme was transformed into whole cells, and 500 g / L L-glutamic acid was added by fed-batch feeding, at 37°C, in disodium hydrogen phosphate-sodium dihydrogen phosphate buffer, pH 7.0 After 12 h of conversion, 347.8 g / L of γ-aminobutyric acid was finally produced, and the molar conversion rate was 99.4%. Compared with the existing glutamic acid decarboxylase, the novel glutamic acid decarboxylase has higher catalytic efficiency and wider pH range, and is more suitable for industrial application.

The invention discloses a preparation method of oxalate decarboxylase. The method comprises the following steps: constructing a recombinant expression plasmid vector comprising Bacillus-containing acid induced promoter, a secretion signal peptide, and a fungus-derived oxalate decarboxylase gene prepared under the control of the secretion signal peptide, transforming bacilli normally growing in a pH value being not more than 4.5 by the vector to prepare oxalate decarboxylase recombinant bacteria; and carrying out culture and acid feeding on the recombinant bacteria in a fermentation medium, inducing the recombinant bacteria, and carrying out simple purification to recover generated extracellular oxalate decarboxylase. The extracellular oxalate decarboxylase prepared through the method is used in diagnosis reagent raw materials, medicinal compositions, healthcare foods or formulated foods with special medical uses. The mutated acid induced promoter is adopted to substitute an original acid induced promoter in the recombinant expression vector, so the transformed bacilli have a high expression level.

The invention relates to the technical field of sewage treatment, in particular to a composite microbial strain for degrading "triphenyl" waste gas and a preservation and activation method thereof. The complex microbial species include Bacillus, Pseudomonas, Streptococcus, Rhodococcus and Brevibacterium. The preservation method comprises the following steps: A1, inoculating the compound microbial strains for degrading "triphenyl" waste gas in a sterile enrichment medium at an inoculum amount of 9-11%, and cultivating by shaking to obtain a bacterial liquid; A2, taking the bacterial liquid , protective agent and active stabilizer, mix well and store at -78-82°C. The composite microbial strain of the present invention can efficiently degrade "triphenyl" waste gas, the preservation method is simple and easy to control, and the cost is low, and the activation of the composite microorganism after preservation can maintain a good "triphenyl" degradation ability, which can be benzene series The practical engineering application of waste gas biological treatment can quickly provide microbial strains with high activity to degrade benzene series.

The invention provides a degrading bactericide for eliminating fluoroglycofen-ethyl residues in wastewater, which belongs to the field of organism high technology. The used bacterial strain is a gram staining reaction negative bacterium FC12 which is identified as bacillus. The main biological property of the gram staining reaction negative bacterium FC12 is G-, the gram staining reaction negative bacterium FC12 is straight rod-shaped, the spore of the gram staining reaction negative bacterium FC12 is elliptical, the peritrichous of the bacillus moves, and the size of the gram staining reaction negative bacterium FC12 is 0.5-2.5*1.2-10 microns; catalase is positive; oxidase is negative; and the gram staining reaction negative bacterium FC12 can be used for hydrolyzing starch and liquefying gelatine. Pesticide residue in water can be reduced by more than 90% by direct application of the degrading bacterium product.

The invention discloses a compound preparation for improving the survival rate of Procambarus clarkii cultured and a use method thereof. The preparation mainly includes component A and component B. Among them, component A is 30-50% of Bacillus and 50-70% of aerobic denitrifying bacteria; component B is 10-15% of quorum effect quencher, 10-15% of synergist, and 20-15% of flocculant 30%, surfactant 10‑15%, oxygenator 20‑30%. In this preparation, component B is used first, and component A is used later after expansion. This preparation can improve the oxidation-reduction potential at the bottom of the aquaculture environment, reduce the reproduction of cyanobacteria and Microcystis aeruginosa in the aquaculture water body, and through the synergistic sequential application of component A and component B, based on the quenching of group sensing effect, microbial ecological occupancy, and microbial matrix The principles of competition and inhibition of microbial metabolites reduce harmful bacteria in the bottom environment and harmful algae in the water body, thereby basically eliminating the production of toxic substances such as algae toxins, hydrogen sulfide, ammonia nitrogen, and nitrite, thereby improving the survival rate of Procambarus clarkii. The preparation has the advantages of fast onset of action and strong effect sustainability.

The invention discloses a preparation method of a portable fish sauce seasoning capsule. The portable fish sauce seasoning capsule comprises components of the portable fish sauce seasoning capsule, and the fish sauce seasoning capsule comprises a capsule shell and content filled in the capsule shell; wherein the capsule shell comprises the following components: 21%-22% of gelatin, 7%-9% of glycerol, 7%-9% of carrageenan, 3%-5% of konjac powder, 3%-5% of locust bean gum, 3%-4% of titanium dioxide powder and the balance of purified water; and the content comprises the following components in percentage by weight: 99.97%-99.99% of fish sauce and 0.01%-0.03% of bacillus subtilis. The bacillus subtilis belongs to aerobic bacteria, enters a dormant state and stops breeding after the oxygen in the capsule is exhausted through mass breeding, so that a stable state is formed, the capsule is convenient to carry and store, can autolyze after being put into hot water condiments, and can also be used for putting granular condiments and counting into a frying pan accurately and conveniently during household cooking; the eating is convenient.