Microbial compound bacteria preparation and preparation method thereof

A technology of microbial compound bacteria and preparations, applied in the field of microbial applications, can solve the problems of difficult purification effect of sewage treatment, and achieve the effect of good growth and reproduction and efficient purification

Active Publication Date: 2021-07-23
马东兵
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex situation of the current urban polluted water and the diversification of pollutants, it is difficult to achieve an effective purification effect in the treatment of sewage by a single microbial strain.

Method used

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  • Microbial compound bacteria preparation and preparation method thereof
  • Microbial compound bacteria preparation and preparation method thereof

Examples

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preparation example Construction

[0036] (1) Preparation of glucose sustained-release granules

[0037] Adding glucose at a final concentration of 60g / L, stearic acid at 20g / L, agar powder at 15g / L and polyethylene glycol 6000 (content ≥ 99%) at 20g / L to deionized water to prepare a mixed solution; Sterilize at 110°C for 30 minutes, drop the mixture into sterile ice-bathed simethicone under aseptic conditions, stir and mix evenly to make particles with a particle size of about 2mm, and remove the particles on the surface of the particles with sterile paper Simethicone, i.e. to obtain sustained-release granules containing glucose.

[0038] Weigh 50 grams of glucose sustained-release granules, place them in 1L of deionized water, take them out every 24 hours at room temperature, dry them and weigh them, and observe their release trend by their weight loss. The results show that, as shown in Table 1 below, the slow-release granules prepared by the present invention release slowly at the beginning, and gradually ...

Embodiment 1

[0043] 1) Bacillus megaterium, Bacillus natto, Pseudomonas aeruginosa, Lactobacillus acidophilus, Torulopsishistolytica, Aspergillus niger, black root Rhizopus nigricans, Penicillium Camemberti, Penicillium Luteum, Mucorracemosus Fres fungi, Trichoderma viride, Thiocapsaroseopersicina , Azotobacter chrococcum (Azotobacter chrococcum) and European nitrosomonas (Nitrosomonas europaea) are activated and expanded in the culture medium comprising yeast extract 5g / L, tryptone 10g / L and sodium chloride 5g / L, obtain expanded cultures;

[0044] 2) According to the weight part of the culture, 75 parts of Bacillus megaterium, 1 part of Bacillus natto, 5 parts of Pseudomonas aeruginosa, 1 part of Lactobacillus acidophilus, 0.5 part of Thiobacillus peach, and Azotobacter torus 3 parts and 2.5 parts of the expanded culture of N. europeanum were inserted into liquid medium 1 according to the preset weight ratio (the formula is as follows: peptone 3g / L, NaCl 5g / L, K 2 HPO 4 0.3g / L and gluc...

Embodiment 2

[0048] According to the same raw materials and steps of Example 1, the microbial composite bacteria preparation was prepared, wherein the various bacterial cultures were mixed according to the following ratios to obtain.

[0049] 65 parts of Bacillus megaterium, 2 parts of Bacillus natto, 9 parts of Pseudomonas aeruginosa, 0.2 parts of Lactobacillus acidophilus, 2.5 parts of Thiobacillus rosea, 1 part of Azotobacter torus, 4 parts of Nitrosomonas europaea , 0.2 parts of Torulopsis histolytica, 2 parts of Aspergillus niger, 0.1 part of Rhizopus niger, 2 parts of Penicillium salmonella casei, 2 parts of Penicillium luteus, 2 parts of Mucor racemosa, 5 parts of Trichoderma viride.

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Abstract

The invention relates to a microbial compound bacterium preparation and a preparation method thereof. The active ingredients of the microbial compound bacteria preparation comprise the following microbial flora of bacillus bacterium, pseudomonas bacterium, lactobacillus bacterium, torulopsis fungus, aspergillus fungus, rhizopus fungus, penicillium fungus, mucor fungus, trichoderma fungus, thiocapsa bacterium, azotobacter bacterium and nitrosmonas bacterium. The microbial compound bacterium preparation disclosed by the invention contains various active bacteria, and through reasonable compatibility of various fungi and bacteria, the microbial compound bacterium preparation exists as a dominant flora in a biological treatment section of sewage treatment, so that the content of pollutant components such as organic pollutants COD, NH3-N, TN and TP and the like in sewage is remarkably reduced, and efficient purification of municipal sewage, domestic garbage leachate wastewater, kitchen and kitchen garbage wastewater, river black and odorous water and industrial wastewater is realized.

Description

technical field [0001] The invention belongs to the technical field of microbial application, and in particular relates to a microbial composite bacterium preparation and a preparation method thereof. Background technique [0002] With the continuous advancement of the urbanization process and the increasing development of the industrial economy, there are more and more domestic sewage, landfill leachate wastewater, kitchen and kitchen waste wastewater, industrial wastewater, black and odorous water bodies in rivers, etc. within the city. Increasingly complex and increasingly serious pollution, and a large number of domestic and industrial sewage continue to flow into rivers and seepage into groundwater. Among them, urban sewage not only contains organic pollutants such as protein, sugar and waste oil, but also contains a large amount of organic and inorganic substances, nitrogen and phosphorus pollution, etc. These pollutions have caused great harm to human health and susta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/16C12N1/20C02F3/34C12R1/66C12R1/80C12R1/845C12R1/885C12R1/88C12R1/01C12R1/07C12R1/065C12R1/38C12R1/225C12R1/785C12R1/465C12R1/02C12R1/645C12R1/125C12R1/10C12R1/11C12R1/085C12R1/82
CPCC12N1/14C12N1/16C12N1/20C02F3/34C02F3/347Y02A50/30
Inventor 马东兵阿部哲弥岳峥王爱平潘晓峰刘学李军王广成叶蓉崔永新
Owner 马东兵
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