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Solid fermentation and application of bacillus algae dissolving bacteria

A Bacillus genus, solid fermentation technology, applied in the direction of bacteria, microorganism-based methods, water/sludge/sewage treatment, etc., can solve problems such as anoxic odor, aquatic animal death, water environment deterioration, etc., and achieve less environmental pollution , strong degradation ability, easy to cultivate effect

Inactive Publication Date: 2012-12-26
ANHUI LIANDA BIOLOGICAL ENVIRONMENTAL PROTECTION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After a large number of microcystic cyanobacteria die in fresh water, the water will be anoxic and foul, and the algae will release toxins, causing a large number of aquatic animals to die, the water environment will be extremely deteriorated, and even humans and animals will be poisoned or die after drinking; this will reduce the ornamental and utilization value of the water body Greatly reduced, causing serious impact on aquaculture, water supply system and ecological environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] First, the Bacillus LDrz1 and LDrz2 strains with algae-dissolving ability isolated by the inventor from the natural water body were purified on the NB agar plate, and then single colonies of LDrz1 and LDrz2 were picked and inoculated into the NB liquid medium at 35 Cultivate the liquid strains under aseptic conditions at ℃; then inoculate the liquid strains with 3% inoculum into the solid medium that has been sterilized at 121°C for 40 minutes and then cooled to 40°C, and in the fermentation room at 30°C Carry out solid fermentation; then dry the fermented material after solid fermentation for 72 hours at 60°C; crush it into algophilic bacteria powder with a fineness of 50 meshes; finally add wheat bran with 100% weight of the bacteria powder to make LDrz1 and LDrz2 single Bacteria, LDrz1+LDrz2 compound algae-lytic bacteria products. The product is diluted with pond water according to the dosage of 5PPM, and then sprinkled in the early stage of eutrophication water body...

Embodiment 2

[0027] Firstly, the Bacillus LDrz1 and LDrz2 strains with algae-dissolving ability isolated by the inventor from natural water were purified on LB agar plates, and then single colonies of LDrz1 and LDrz2 were picked and inoculated into LB liquid medium at 35°C. Cultivate the liquid strains under sterile conditions; then inoculate the liquid strains with 8% inoculum into the solid medium that has been sterilized at 121°C for 40 minutes and then cooled to 40°C in the fermentation room at 32°C Solid fermentation; then dry the fermented material after solid fermentation for 48 hours at 70°C; crush it into algophilic bacteria powder with a fineness of 50-60 meshes; finally add 100% wheat bran by weight and 10% wheat bran by weight to the bacteria powder r-PGA (biological flocculant) made of LDrz1, LDrz2 single bacteria, LDrz1+LDrz2 composite bacteria modified algicidal bacteria products (after the modified product is applied to the water body, r-PGA can promote the flocculation of a...

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Abstract

The invention discloses a solid fermentation and application of bacillus algae dissolving bacteria. Bacillus LDrz1 and LDrz2 bacterial strains with algae dissolving bacteria energy and preserved in a laboratory of a company are purified, and then liquid strains are cultured on the aseptic condition of 30-37 DEG C; the liquid strains are inoculated to solid media after sterilization to be in solid fermentation on the condition of 30-37 DEG C; fermentation materials after 36-48 hour solid fermentation are dried on the condition of 60-70 DEG C; algae dissolving bacteria raw powder with the fineness of 50-60 microns are obtained by grinding; and according to requirements, carrier materials are added to prepare single bacteria or compound bacteria algae dissolving bacteria products or modified algae dissolving bacteria products. The products are used in the early period of origination of a eutrophic body of water, algae biomass can be controlled or the algae is prevented from large-scale multiplication in a short period of time, water transparency is quickly improved, recovery of other animals or plants in the water is promoted, ecological balance of the water environment is finally achieved, and the solid fermentation and the application of the bacillus algae dissolving bacteria are an important means of water bloom biological control.

Description

technical field [0001] The invention belongs to the field of biological treatment of water environment pollution, and in particular relates to solid fermentation and application of algae-lytic bacteria belonging to the genus Bacillus. Background technique [0002] Eutrophication of water body is a global environmental problem with extensive influence. The phenomenon of large-scale algal blooms in a short period of time in fresh water is called "lake indigo" (or "bloom"), and it is called "red tide" in seawater. [0003] With the impact of human beings on the natural environment, the degree of eutrophication in water bodies is getting higher and higher, which leads to a large number of micro-phytoplankton algae in water bodies, especially in high temperature seasons. After a large number of microcystic cyanobacteria die in fresh water, the water will be anoxic and foul, and the algae will release toxins, causing a large number of aquatic animals to die, the water environme...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/07
Inventor 朱杰周呈祥吴文坤
Owner ANHUI LIANDA BIOLOGICAL ENVIRONMENTAL PROTECTION TECH
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