Compound preparation for improving the survival rate of Procambarus clarkii cultured and its application method

A technology of Crayfish and survival rate, applied in the field of compound biological preparation preparation, can solve problems such as poor application effect, failure of breeding, neglect of means of overall environmental regulation, etc., so as to improve redox potential, increase efficiency, and maintain high quality The effect of bottom and water environment

Active Publication Date: 2021-08-24
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the biological agents in the field of Procambarus clarkii adjust the breeding environment from the perspective of "individual microorganisms", and the products are mostly single or compound strains, ignoring the means to achieve overall environmental regulation based on microbial ecology and "microbial community attributes" , so it often leads to poor application effect or even breeding failure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Application case of Procambarus clarkii cement pond culture. The present embodiment includes component A and component B, wherein component A is 25% of Bacillus subtilis, 25% of Bacillus coagulans, 25% of Pseudomonas, and 25% of Marinobacterium excluding hydrocarbons. Component B is vanillin 18%, acetyltransferase 17%, activated carbon powder 15%, polyacrylamide 20%, sodium lauryl sulfate 15%, calcium peroxide 15%. The dosage of component B is 15 mg / m 3 Water body; the dosage of component A after expansion is 5 mL / m 3 Water body.

[0031] Table one is the effect that this embodiment is applied to Procambarus clarkii cement pond cultivation:

[0032] Table 1. Application effect of compound preparation cement pool

[0033] project value NH before administration 4 + -N (mg / L)

[0034] Remarks: a, the breeding process was 120 days, and the compound preparation was used 3 times, with an average interval of 40 days; b, except for the compound prepar...

Embodiment 2

[0036] Application case of Procambarus clarkii cultured in earthen ponds. The present embodiment includes component A and component B, wherein component A is 15% of Bacillus subtilis, 17% of Bacillus coagulans, 33% of Pseudomonas, and 35% of Marinobacter alba. Component B is vanillin 12%, acetyltransferase 13%, sodium nitrate 10%, activated carbon powder 10%, calcium hydroxide 5%, polyacrylamide 20%, sodium lauryl sulfate 15%, sodium percarbonate 15% %. The dosage of component B is 5 mg / m 3 Water body; the dosage of component A after expansion is 3 mL / m 3 Water body.

[0037] Table two is the effect that this embodiment is applied to Procambarus clarkii earthen pond culture:

[0038] Table 2. Application effect of compound preparation earth pond

[0039] project value NH before administration 4 + -N (mg / L)

[0040] Remarks: a, the breeding process was 147 days, and the compound preparation was used 3 times, with an average interval of 50 days; b, ex...

Embodiment 3

[0042]Application case of Procambarus clarkii in paddy fields. This embodiment includes component A and component B, wherein component A is 20% of Bacillus subtilis, 30% of Bacillus coagulans, 25% of Pseudomonas, and 25% of Marinobacterium dehydrocarbon. Component B is vanillin 5%, acetyltransferase 5%, sodium nitrate 10%, activated carbon powder 10%, calcium hydroxide 10%, polyacrylamide 30%, sodium lauryl sulfate 15%, sodium percarbonate 15% %. The dosage of component B is 7 mg / m 3 Water body; the dosage of component A after expansion is 4 mL / m 3 Water body.

[0043] Table three is the effect that this embodiment is applied to Procambarus clarkii paddy field cultivation:

[0044] Table 3. Application effect of compound preparation in paddy field culture

[0045] project value NH before administration 4 + -N (mg / L)

[0046] Remarks: a, the breeding process was 160 days, and the compound preparation was used 3 times, with an average interval of 50 d...

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Abstract

The invention discloses a compound preparation for improving the survival rate of Procambarus clarkii cultured and a use method thereof. The preparation mainly includes component A and component B. Among them, component A is 30-50% of Bacillus and 50-70% of aerobic denitrifying bacteria; component B is 10-15% of quorum effect quencher, 10-15% of synergist, and 20-15% of flocculant 30%, surfactant 10‑15%, oxygenator 20‑30%. In this preparation, component B is used first, and component A is used later after expansion. This preparation can improve the oxidation-reduction potential at the bottom of the aquaculture environment, reduce the reproduction of cyanobacteria and Microcystis aeruginosa in the aquaculture water body, and through the synergistic sequential application of component A and component B, based on the quenching of group sensing effect, microbial ecological occupancy, and microbial matrix The principles of competition and inhibition of microbial metabolites reduce harmful bacteria in the bottom environment and harmful algae in the water body, thereby basically eliminating the production of toxic substances such as algae toxins, hydrogen sulfide, ammonia nitrogen, and nitrite, thereby improving the survival rate of Procambarus clarkii. The preparation has the advantages of fast onset of action and strong effect sustainability.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and specifically relates to a compound biological agent preparation technology and a use method for improving the survival rate of Procambarus clarkii culture by improving the culture bottom and water body environment. Background technique [0002] Procambarus clarkii ( Procambarus clarkia ), commonly known as crayfish, is a product that is deeply loved by consumers. At present, it has become an important species in rice field farming and pond farming in my country, and it also provides effective support for ensuring the safety of vegetable baskets. [0003] However, due to the large amount of high-protein feed fed during the breeding process, it is very easy to cause toxic ammonia nitrogen, hydrogen sulfide, nitrite and other substances in the bottom environment. At the same time, the residual nutrient elements are in the water body, and the high temperature season will easily lead to the o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/59C12N1/20C02F3/34C12R1/125C12R1/07C12R1/38C12R1/01C02F103/20
CPCA01K61/59C02F3/34C02F3/348C02F2103/20C02F2303/04C02F2305/04C12N1/20Y02A40/81
Inventor 阮贇杰周凡丁雪燕徐向阳孔德栋殷旭旺蔡磊陆慧锋
Owner ZHEJIANG UNIV
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